2012 Fiscal Year Final Research Report
Improvement of somatic cell nuclear transfer techniques in miniature pigs using a noninvasive evaluation system for reprogramming status
| Project/Area Number |
22580321
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Kagoshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SATO Masahiro 鹿児島大学, 医用ミニブタ・先端医療開発 研究センター, 教授 (30287099)
YOSHIDA Mitsutoshi 鹿児島大学, 農学部, 教授 (00174954)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 体細胞クローニング / 核移植 / リプログラミング / ミニブタ |
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| Research Abstract |
It was indicated that treatment with some histone deacetylase inhibitors, such as valproic acid, suberoyl bishydroxamic acid and scriptaid, is beneficial for the efficient production of miniature pig somatic cell nuclear transfer embryos using a noninvasive evaluation system for the reprogramming status of donor nuclei on the basis of Oct-3/4 expression. In addition, an alive transgenic miniature pig expressing human apolipoprotein(a), a risk factor for the development of atherosclerosis, was successfully obtained using treatment with scriptaid.
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