2012 Fiscal Year Final Research Report
Identification of genes involved in Na/K ratio-independent growth of Melissococcus plutonius.
| Project/Area Number |
22580345
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
TAKAMATSU Daisuke 独立行政法人農業・食品産業技術総合研究機構, 動物衛生研究所細菌・寄生虫研究 領域, 主任研究員 (60414728)
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| Co-Investigator(Renkei-kenkyūsha) |
OSAKI Makoto 独立行政法人農業・食品産業技術総合研究機構, 動物衛生研究所細菌・寄生虫研究 領域, 主任研究員 (80355164)
OKURA Masatoshi 独立行政法人農業・食品産業技術総合研究機, 構動物衛生研究所細菌・寄生虫研究 領域, 研究員 (60508315)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 病原微生物 / ヨーロッパ腐蛆病菌 / カリウム要求性 |
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| Research Abstract |
Melissococcus plutonius is a fastidious honeybee pathogen, and the addition of KH2PO4 to culture medium is required for the growth of typical M. plutonius strains isolated in the world. In contrast, atypical strains isolated only in Japan do not show potassium requirement for the growth. Using genome sequences and a newly developed vector, we showed that mutations in genes encoding Na^+/H^+ antiporter and cation-transporting ATPase are involved in the potassium requirement for the growth of typical M. plutonius strains.
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[Journal Article] Diversity of Melissococcus plutonius from Honeybee Larvae in Japan and Experimental Reproduction of European Foulbrood with Cultured Atypical Isolates2012
Author(s)
Rie Arai, Kiyoshi Tominaga, Meihua Wu, Masatoshi Okura, Kazutomo Ito, Naomi Okamura, Hidetaka Onishi, Makoto Osaki, Yuya Sugimura, Mikio Yoshiyama, Daisuke Takamatsu
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Journal Title
PLoS One
Volume: Vol. 7
Pages: e33708
Peer Reviewed
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[Presentation] Phenotypic and genetic diversity of Melissococcus plutonius isolates from honeybee larvae in Japan and experimental reproduction of European foulbrood by cultured bacteria., ASM20122012
Author(s)
Daisuke Takamatsu, Rie Arai, Kiyoshi Tominaga, Meihua Wu, Masatoshi Okura, Kazutomo Ito, Naomi Okamura, Hidetaka Onishi, Makoto Osaki, Yuya Sugimura,Mikio Yoshiyama
Organizer
112th General Meeting of the American Society for Microbiology
Place of Presentation
Moscone Center, San Francisco (USA)
Year and Date
20120616-19
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