2012 Fiscal Year Final Research Report
Highly Assay of Autoantigens in BSE Sera for a Diagnosis
| Project/Area Number |
22580353
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied veterinary science
|
|---|
| Research Institution | Rakuno Gakuen University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Keywords | BSE / 生前診断 / GFAP / 血清蛋白質 / 質量分析 |
|---|
| Research Abstract |
Our objective is to detect a serum biomarker Glial Fibrillary Acidic Protein (GFAP) of Bovine Spongiform Encephalopathy (BSE) by the Selected Reaction Monitoring (SRM) of quadrupole MS analysis from a bovine serum. SRM conditions were determined from the data of MS/MS fragmentation and identification of the protein by data dependent MS/MS analysis of Q-pole MS. An additive GFAP was highly recovered by the preparation method using alkylation of the proteins and removal of the albumin from the sera. SRM methods reported in this study could identify protein and peptide with high selectivity and sensitivity, therefore this method was useful for screening of known proteins and peptides.
|
Research Products
(11 results)
-
-
-
-
-
-
-
-
-
-
[Journal Article] Neutrophil elastase inhibitor improves survival of rats with clinically relevant sepsis2010
Author(s)
Suda K, Takeuchi H, Hagiwara T, Miyasho T, Okamoto M, Kawasako K, Yamada S, Suganuma K, Wada N, Saikawa Y, Fukunaga K, Funakoshi Y, Hashimoto S, Yokota H, Maruyama I, Ishizaka A,Kitagawa Y
-
Journal Title
Shock. May
Volume: 33(5):526-531
Peer Reviewed
-
