2012 Fiscal Year Final Research Report
Analysis of phosphorylated biomolecules using a phosphate-affinity quartz-crystal microbalance method
| Project/Area Number |
22590037
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Hiroshima University |
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Principal Investigator |
KINOSHITA Eiji 広島大学, 大学院・医歯薬保健学研究院, 准教授 (80304418)
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| Project Period (FY) |
2010 – 2012
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| Keywords | リン酸化 / 水晶発振子マイクロバランス / QCM / バイオセンサー / フォスタグ / 分子認識 / 蛋白質 / 分析化学 |
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| Research Abstract |
In this study, the researcher has applied a novel phosphate capture molecule, biotinylated Phos-tag (Phos-tag Biotin BTL-111), which was originally synthesized, to a quartz-crystal microbalance procedure (QCM). By using the BTL-111，a phosphate-affinity QCM sensor chip has been newly developed. This novel biosensor chip permitted highly sensitive detections of phosphorylated biomolecules, such as phosphoproteins or DNA.
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[Journal Article] A Phos-tag-based magnetic-bead method for rapid and selective separation of phosphorylated biomolecules2013
Author(s)
Tsunehiro, M., Meki, Y., Matsuoka, K., Kinoshita-Kikuta, E., Eiji Kinoshita, and Koike, T
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Journal Title
J. Chromatogr
Pages: 86-94
DOI
Peer Reviewed
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[Journal Article] Protein phosphorylation in encystment-induced Colpoda cucullus: Localization and identification of phosphoproteins.2012
Author(s)
Sogame, Y., Kojima, K., Takeshita, T., Fujiwara, S., Miyata, S., Eiji Kinoshita, and Matsuoka, T
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Journal Title
FEMS Microbiol. Lett
Volume: 331
Pages: 128-135
DOI
Peer Reviewed
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[Journal Article] Highly sensitive detection of protein phosphorylation by using improved Phos-tag Biotin2012
Author(s)
Eiji Kinoshita, Kinoshita-Kikuta, E., Sugiyama, Y., Fukada, Y., Ozeki, T., and Koike, T
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Journal Title
Proteomics
Volume: 12
Pages: 932-937
DOI
Peer Reviewed
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