photochemistry of sensory rhodopsin

2012 Fiscal Year Final Research Report

• Project/Area Number: 22590049
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Hokkaido University (2012); Matsuyama University (2010-2011)
• Principal Investigator: KAMO Naoki 北海道大学, 名誉教授 (10001976)
• Co-Investigator(Kenkyū-buntansha): NARA Toshifumi 松山大学, 薬学部, 准教授 (30241350)
• Project Period (FY): 2010 – 2012
• Keywords: レチナールタンパク / 微生物ロドプシン / ホボロドプシン / ホトサイクルヒロロキシルアミン / レチナール異性化 / 高度好塩菌

Research Abstract

In the membrane of Halobaterium salinarum, there exists sensory rhodopsin (SR) which acts as a photoreceptor of phototaxis of this bacterium. Photo-induced proton transfer of SR was made clear. So-called M-intermediate is considered to be functionally important, but we showed that this intermediate is fragile. We devised the apparatus which can monitor time-resolved photo-induced proton transfer. We first showed a so-called N-intermediate in SR. The photochemistry of SRIII was first clarified.

Research Products

• [Journal Article] Exporing the active site site structure of a photoreceptor protein by raman optical activity (2013)
  • Author(s): Unno Masashi, kikukawa takashi, Kumauchi Masato, Kamo Naoki
  • Journal Title: Journal of Physical Chemistry. Volume: 117巻 Pages: 1321-325
  • DOI: doi:10.1021/jp4001187
  • Peer Reviewed
• [Journal Article] Thermodynamic parameters of anionbinding to halorhodopsin from Natronomonas pharaonis by isothermal titration calorimetry (2013)
  • Author(s): Hayasi Saori, Tamogami Jun, Kikukawa Takashi, Okamoto Haruka, Shimono Kazumi, Miyauchi Seiji, Demura Makoto, Nara To shifumiKamo Naoki
  • Journal Title: Biophysical Chemistry Volume: 172巻 Pages: 61-6710
  • DOI: doi:1016/j.bpc.2013.01.001
  • Peer Reviewed
• [Journal Article] Photoinduced proton release in proteorhodopsin at low pH: The possibility of a decrease in the pKa of Asp227 (2012)
  • Author(s): Tamogami Jun, Kikukawa Takashi, NaraToshifumi , Shimono Kazumi, Demura Makoto, Kamo Naoki
  • Journal Title: Biochemistry Volume: 51巻 Pages: 9290-9301
  • DOI: doi:10.1021/bi300940p
  • Peer Reviewed
• [Journal Article] Photo-induced bleaching of sensory rhodopsin II (phoborhodopsin) from Halobaterium salinarum by hydroxylamine: Identification of the responsible intermediates. (2012)
  • Author(s): Tamogami Jun, Kikukawa Takashi, IkedaYoichi, Demura Makoto, NaraToshifumi , KamoNaoki
  • Journal Title: Journal of Photochemistry and Photobiology B-Biology Volume: 106巻 Pages: 87-9410
  • DOI: doi:1016/j.photobiol.2011.10.09
  • Peer Reviewed
• [Journal Article] Ramamoorthy Ayyalusamy, Naito Akira. An active photoreceptor intermediate revealed by in situ photoirradiated solid-state NMR spectroscopy (2011)
  • Author(s): Tomonaga Yuya, Hidaka Tetsuro, Kawamura Izuru, Nishio Takudo, Osawa Kazuhiro, Okitsu Takashi, Wada Akimori, Sudo Yuki, KamoNaoki
  • Journal Title: Biophysical Journal Volume: 101巻 Pages: L50-L5210
  • DOI: doi:1016/j.bpj.2011.10.022
  • Peer Reviewed
• [Journal Article] An amino acid residue (S201) in the retinal binding pocket regulates the photoreaction pathway of phoborhodopisin (2011)
  • Author(s): Dai Gang, Zhang Yu, Tamogami Jun, Demura Makoto, N ao k i Ka m o , Kandori Hideki, Iwasa Tatsuo
  • Journal Title: Biochemistry Volume: 50巻 Pages: 7177-7183
  • DOI: doi:10.1021/bi200598r
  • Peer Reviewed
• [Journal Article] Spectroscopic evidence for the formation of an N-intermediate during the photocycle of sensory rhodopsin (phoborhodopsin) from Natromonaspharaonis (2011)
  • Author(s): Tateishi Yusuke, Abe Takayuki, Tamogami Jun, Nakao Yutaka, Kikukawa Takashi, KamoNaoki , Unno Masashi
  • Journal Title: Biochemistry Volume: 50巻 Pages: 2135-2143
  • DOI: doi:10.1021/bi1019572
  • Peer Reviewed
• [Presentation] When halorhodopsin does release anion (2012)
  • Author(s): Kikukawa, T., Kokubo, A., Tsukamoto, T., Ihara, K., Kamo , N ., Demura, N
  • Organizer: 15th International Conference on Retinal Proteins
  • Place of Presentation: Monte Verita, Ascona, (Switzerland)
  • Year and Date: 2012-10-15
• [Presentation] The role of chloride in the photo-induced proton transfer in sensory rhodopsin II from Natronomonas pharaoni (2012)
  • Author(s): Tamogami, J., Iwano, K., Matsuyama, A., Kikukawa, T., Demura, M., Shimono, K., Nara, T. , Kamo , N
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: 名古屋大学(名古屋市)
  • Year and Date: 2012-09-23
• [Presentation] 海洋細菌由来のロドプシン様タンパク質プロテオロドプシンの低pH条件下における速いプロトン放出機構 (2011)
  • Author(s): 田母神淳、菊川峰志,下野和実,奈良敏文,宗行英朗,加茂直樹
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2011-03-30
• [Presentation] Laser flash analysis of functional interaction between sensory rhodopsin NpSRII (ppR) and eubacterial chemoreceptor Tsr in E. coli (2010)
  • Author(s): 奈良敏文、菊川峰志、田母神淳、 加茂直樹
  • Organizer: 第48回日本生物物理学会
  • Place of Presentation: 東北大学(仙台市)
  • Year and Date: 2010-09-20
• [Presentation] センサリーロドプシンIIのヒドロキシルアミンによる退色反応 (2010)
  • Author(s): 田母神淳、菊川峰志、池田陽一、出村誠、加茂直樹
  • Organizer: 第10回蛋白質科学会年会
  • Place of Presentation: 札幌コンベンションセンター(札幌市)
  • Year and Date: 2010-06-18
• [Presentation] センサリーロドプシンIIのヒドロキシルアミンによる退色反応 (2010)
  • Author(s): 田母神淳、菊川峰志、池田陽一、出村誠、加茂直樹
  • Organizer: 題10回蛋白質科学会年会
  • Place of Presentation: 札幌コンベンションセンター(札幌市)
  • Year and Date: 2010-06-18
• [Remarks] 現在名誉教授なので、ホームページはない。一昨年度まで在籍していた松山大学
  • URL: http://ghp01.matsuyama-u.ac.jp/~yakugaku/laboratory/labo_biophysical-chemistry.html