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2012 Fiscal Year Final Research Report

Development of lower-interactive, stable, brighter near-infrared fluorescent dyes and their application to bio-imaging

Research Project

  • PDF
Project/Area Number 22590051
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Physical pharmacy
Research InstitutionFukuoka University

Principal Investigator

NOHTA Hitoshi  福岡大学, 薬学部, 教授 (20164668)

Project Period (FY) 2010 – 2012
Keywordsイメージング / 蛍光色素
Research Abstract

Fluorescence analyses play an important role in progress of biosciences, but the fluorescence dyes remain unchanged for long time. This study aimed to develop lower-interactive, stable, water-soluble, highly fluorescent dyes. The developed novel dyes “glycated rohdamines” show unique features “highly water-soluble and lower-interactivity with bio-molecules”. This study afford a typical methodology for developing new fluorescent dyes.

  • Research Products

    (15 results)

All 2013 2012 2011

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (5 results) Book (2 results)

  • [Journal Article] Fully Automated Reagent Peak free LC Fluorescence Analysis of Highly Polar Carboxylic Acids Using a Column-switching System and Fluorous Scavenging-derivatization2013

    • Author(s)
      Kenichiro Todoroki
    • Journal Title

      Journal of Separation Science、

      Volume: 36/2 Pages: 232-238

    • Peer Reviewed
  • [Journal Article] LCdetermination of Microcystins in WaterSamples Following Pre-column ExcimerFluorescence Derivatization with4-(1-Pyrene)butanoic acid hydrazide2012

    • Author(s)
      Hayama Tadashi
    • Journal Title

      Analytica Chimica Acta

      Volume: 755 Pages: 93-99

    • Peer Reviewed
  • [Journal Article] Binary Fluorous Alkylation of Biogenic Primary Amines with Perfluorinated Aldehyde Followed by Fluorous LC-Tandem MS Analysis2012

    • Author(s)
      Hayama Tadashi
    • Journal Title

      Analytica Ceimistry

      Volume: 84/19 Pages: 8407-8414

    • Peer Reviewed
  • [Journal Article] Determination of Histamine in Micro- dialysis Samples from Guinea Pig Skin byHPLC with Fluorescence Detection2012

    • Author(s)
      Takashi Yoshitake
    • Journal Title

      SkinPharmacology and Physiology

      Volume: 25/2 Pages: 65-72

    • Peer Reviewed
  • [Journal Article] Selective Liquid-Chromatographic Determination of Native Fluorescent Biogenic Amines in Human Urine Based on Fluorous Derivatization2011

    • Author(s)
      Soiciro Ijiri
    • Journal Title

      Electrophoresis

      Volume: 32/24 Pages: 3499-3509

    • Peer Reviewed
  • [Journal Article] Binary Fluorous Alkylation of Biogenic Primary Amines with Perfluorinated Aldehyde Followed by Fluorous LC-Tandem MS Analysis2011

    • Author(s)
      Yohei Sakaguchi
    • Journal Title

      Journal of Chromatography A

      Volume: 1218/33 Pages: 5581-5586

    • Peer Reviewed
  • [Journal Article] Highly Sensitive and Selective Derivatization-LC Method for Biomolecules Based on Fluorescence Interactions and Fluorous Separations2011

    • Author(s)
      Kenichiro Todoroki
    • Journal Title

      Journal of Chromatography B

      Volume: 879/17-18 Pages: 1325-1337

    • Peer Reviewed
  • [Journal Article] Selective Determination of Cysteines through Precolumn Double-labeling and Liquid Chromatography Followed by Detection of Intramolecular FRET2011

    • Author(s)
      Makoto Yoshitake
    • Journal Title

      Analytical and Bioanalytical Chemistry

      Volume: 399/4 Pages: 1665-1675

    • Peer Reviewed
  • [Presentation] アミノ酸メタボロミクスを用いた抗がん剤の治療効果判定法の開発(3)2013

    • Author(s)
      富田陵子
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      横浜市
    • Year and Date
      2013-03-28
  • [Presentation] フルオラスイオンペア剤を利用したリン酸基含有化合物の選択的抽出法の開発2013

    • Author(s)
      巴山忠
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      横浜市
    • Year and Date
      2013-03-28
  • [Presentation] フ還元的ジフルオラスアルキル化による生理活性アミン類の高選択的LC-MS/MS分析2012

    • Author(s)
      坂口洋平
    • Organizer
      第72回分析化学討論会
    • Place of Presentation
      鹿児島市
    • Year and Date
      2012-05-19
  • [Presentation] Fluorous Derivatization Combined with LC-MS/MS: Matrix Effect-Free Method forThe Determination of Biogenic Amines2011

    • Author(s)
      巴山忠
    • Organizer
      IUPACInternational Congress on Analytical Sciences2011
    • Place of Presentation
      京都市
    • Year and Date
      2011-05-22
  • [Presentation] Analysis of Sialo-Sugar Chains Using Fluorous Derivatization Followed by Liquid Chromatography with Tandem MassSpectrometry2011

    • Author(s)
      坂口洋平
    • Organizer
      IUPAC International Congress on Analytical Sciences 2011
    • Place of Presentation
      京都市
    • Year and Date
      2011-05-22
  • [Book] エキシマー蛍光誘導体化による高感度生体アミン分析2012

    • Author(s)
      能田均
    • Total Pages
      112-126
  • [Book] 脳内神経伝達物質の高感度リアルタイム分析2012

    • Author(s)
      能田均
    • Total Pages
      168-85

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Published: 2014-08-29  

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