Linkage of neuronal morphology to gene expression:actin-bound transcription cofactor MKL and its regulation by BDNF

2012 Fiscal Year Final Research Report

• Project/Area Number: 22590080
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: University of Toyama
• Principal Investigator: TABUCHI Akiko 富山大学, 大学院・医学薬学研究部(薬学), 准教授 (40303234)
• Project Period (FY): 2010 – 2012
• Keywords: 遺伝子発現 / 転写因子 / 脳由来神経栄養因子(BDNF) / SRF / Arc

Research Abstract

Activity-regulated cytoskeleton-associated protein （Arc) is a regulator of neuronal function. In this study, we have found that SRF cofactors, megakaryoblastic leukemia 1（MKL1) and Ets-like transcription factor 1 （Elk1), activate and inhibit Arc gene transcription, respectively. These findings, at least in part, contribute to a better understanding of brain function and provide a novel insight into SRF cofactor-based drug design.

Research Products

• [Journal Article] Synthesis and biological evaluation of pyrethroid insecticide-derivatives as a chemical inducer for Bdnf mRNA expression in neurons (2012)
  • Author(s): Matsuya, Y., Ihara, D.,Fukuchi, M., Honma, D., Itoh, K., Tabuchi, A., Nemoto, H. and Tsuda, M
  • Journal Title: Bioorg. Med. Chem Volume: 20 Pages: 2564-2371
  • Peer Reviewed
• [Journal Article] Deltamethrin, a type II pyrethroid insecticide, nas neurotrophic effects on neurons with continuous activation of the Bdnf promoter (2012)
  • Author(s): Ihara, D., Fukuchi, M., Honma, D., Takasaki, I., Ishikawa, M., Tabuchi, A. and Tsuda, M
  • Journal Title: Neuropharmacology Volume: 62 Pages: 1091-1098
  • Peer Reviewed
• [Journal Article] Up-regulation of L-type amino acid transporter 1 (LAT1) in cultured rat retinal capillary endothelial cells in response to glucose deprivation. Drug (2012)
  • Author(s): Matsuyama, R., Tomi, M., Akanuma, S., Tabuchi, A., Kubo, Y., Tachikawa, M. and Tsuda M
  • Journal Title: Metab. Pharmacokinet Volume: 27 Pages: 317-324
  • Peer Reviewed
• [Journal Article] Activation of tyrosine hydroxylase (TH) gene transcription induced by brain-derived neurotrophic factor (BDNF) and its selective inhibition through Ca(2+) signals via the N-methyl-D-aspartate (NMDA) receptor (2010)
  • Author(s): Fukuchi, M., Fujii, H., Takachi, H., Ichinose, H., Kuwana, Y., Tabuchi, A. and Tsuda M
  • Journal Title: Brain Res Volume: 1366 Pages: 18-26
  • Peer Reviewed
• [Journal Article] Pituitary adenylate cyclase-activating polypeptide (PACAP) is an upstream regulator of prodynorphin mRNA expression in neurons (2010)
  • Author(s): Dong, Y. X., Fukuchi, M., Inoue, M., Takasaki, I., Tabuchi, A., Wu, C. F. and Tsuda M
  • Journal Title: Neurosci, Lett Volume: 484 Pages: 174-177
  • Peer Reviewed
• [Journal Article] Involvement of the serum response factor coactivator megakaryoblastic leukemia (MKL) in the activin-regulated dendritic complexity of rat cortical neurons (2010)
  • Author(s): Ishikawa, M., Nishijima, N., Shiota, J., Sakagami, H., Tsuchida, K., Mizukoshi, M., Fukuchi, M., Tsuda, M. and Tabuchi, A
  • Journal Title: J. Biol. Chem Volume: 285 Pages: 32734-32743
  • Peer Reviewed
• [Presentation] アクチン結合性転写制御因子MKL1アイソフォームのラット脳組織における発現および機能解析 (2012)
  • Author(s): 石橋悠太*, 石川充, 塩田惇, 袴田知之, 庄司しずく, 福地守, 津田正明, 田渕明子
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 20121211-14
• [Presentation] ラット大脳皮質ニューロンでのBDNF誘導性Arc/Arg3.1遺伝子発現における転写因子SRFコファクター間の競合的関係 (2012)
  • Author(s): 石川充, 福地守, 菊池啓悦, 石橋悠太, 辻井惇也, 津田正明, 奥野浩行, 尾藤晴彦, 田渕明子
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 20121211-14
• [Presentation] G-protein-coupled receptor-mediated activity-dependent gene expression via NMDA receptors in neurons (2012)
  • Author(s): Fukuchi M., Tabuchi A., Takasaki I., Takemori H., and Tsuda M
  • Organizer: Neuroscience 2012 (Society for Neuroscience's 42nd annual meeting)
  • Place of Presentation: NewOrleands, USA
  • Year and Date: 20121013-17
• [Presentation] アクチン結合性転写活性化因子MKL1のリン酸化による SRF介在性遺伝子発現制御 (2012)
  • Author(s): 石橋悠太*, 辻井惇也, 石川充, 福地守, 津田正明, 田渕明子
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 20120918-21
• [Presentation] アクチン結合性転写活性化因子MKL1の新規スプライスバリアントの同定とラット大脳皮質ニューロンにおける機能解析 (2012)
  • Author(s): 石川充, 塩田惇, 袴田知之, 石橋悠太, 庄司しずく, 福地守, 津田正明, 田渕明子
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 20120918-21
• [Presentation] dentification and characterization of a novel transcript of rat MKL1, the actin-binding coactivator for SRF: the effect on transcriptional activation and neuronal morphology in rat cortical neurons (2011)
  • Author(s): 石川充, 塩田惇, 袴田知之, 石橋悠太, 庄司しずく, 野村未希, 福地守, 津田正明, 田渕明子
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20111213-16
• [Presentation] 脳の発達段階におけるMKL/SRF転写抑制因子SCAIの発現分布 (2011)
  • Author(s): 水越美帆*, 石川充, 皆藤真季, 福地守, 津田正明, 田渕明子
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 20110914-17
• [Presentation] アクチン結合性転写活性化因子MKLが関与するアクチビン誘導性のラット大脳皮質ニューロン樹状突起形態制における新規シグナル伝達経路の解析 (2011)
  • Author(s): 石川充, 西嶋直紀, 阪上洋行, 土田邦博, 水越美帆, 塩田惇, 福地守, 津田正明, 田渕明子
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 20110914-17
• [Presentation] 脳由来神経栄養因子BDNFによって誘導されるSRFコアクチベーターMKL1のリン酸化修飾 (2011)
  • Author(s): 田渕明子, 石橋悠太, 辻井惇也, 石川充, 福地守, 津田正明
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 20110914-17
• [Presentation] Differential expression of rat SRF coactivator MKL1 transcripts during brain development: the possible involvement in dendritic morphology (2011)
  • Author(s): Tabuchi A
  • Organizer: The 6th International Conference of Neurons and Brain Diseases
  • Place of Presentation: Toyama.
  • Year and Date: 20110803-05
• [Presentation] アクチン結合性転写活性化因子MKLはアクチビンに誘導される転写活性および樹状突起形態の変化に関与する (2010)
  • Author(s): 石川充*, 西嶋直紀, 塩田惇, 阪上洋行, 土田邦博, 水越美帆, 福地守, 津田正明, 田渕明子
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 20101207-10
• [Presentation] MKL, an actin-binding coactivator for SRF, is involved in TGF-βfamily-induced alteration of dendritic morphology and transcriptional activity in rat cortical neurons (2010)
  • Author(s): Ishikawa M.*, Nishijima N., Shiota J., Sakagami H., Tsuchida K., Tsuda M., Tabuchi A
  • Organizer: Neuroscience 2010 (The 40th annual meeting of the Society for Neuroscience)
  • Place of Presentation: SanDiego, USA
  • Year and Date: 20101113-17
• [Presentation] Isolation and characterization of rat SRF coactivator MKL1 splice variants differentially expressed in the developing brain (2010)
  • Author(s): Tabuchi A., Hakamata T., Ishikawa M., Shiota J., Shoji S., Fukuchi M., Tsuda M
  • Organizer: Neuroscience 2010 (The 40th annual meeting of the Society for Neuroscience)
  • Place of Presentation: SanDiego, USA
  • Year and Date: 20101113-17
• [Presentation] アクチン結合性転写因子MKL1のラットスプライスバリアントの同定 (2010)
  • Author(s): 田渕明子, 袴田知之, 石川充, 塩田惇, 庄司しずく, 津田正明
  • Organizer: 第33回日本神経科学大会・第53回日本神経化学会大会・第20回日本神経回路学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 20100902-03
• [Book] 化学同人「プロッパー細胞生物学」 (2013)
  • Author(s): 中山和久(監訳)、田渕明子(第11章担当)
  • Total Pages: 29