Studies on signaling pathways mediated by Nucling, a novel apoptosis-associating protein, in the development of inflammatory disorders and tumors

2012 Fiscal Year Final Research Report

• Project/Area Number: 22590286
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: The University of Tokushima
• Principal Investigator: SAKAI Takashi 徳島大学, 疾患酵素学研究センター, 准教授 (80284321)
• Co-Investigator(Kenkyū-buntansha): FUKUI Kiyoshi 徳島大学, 疾患酵素学研究センター, 教授 (00175564)
• Project Period (FY): 2010 – 2012
• Keywords: ヌクリング / NF-κB / アポトーシス / 炎症性疾患 / 発ガン

Research Abstract

We revealed that Nucling is important for the strict regulationof NF-・B signal in resting state. Aberrant activation of NF-・B in Nucling-deficient tissues or cells showed defect of apoptosis pathways resulted in the resistance against cytotoxic stress. It is possibly one of the factors for the high prevalence ratios of hepatitis and hepatic cancer. These results will lead to the elucidation of the regulatory mechanism of the pathogenesis of the diseases or to the development of novel therapeutic strategies.

Research Products

• [Journal Article] Nucling, a novel protein associated with NF-・B, regulates endotoxin-induced apoptosis in vivo (2013)
  • Author(s): Sun Mi Kim, Sakai, T., Huy Van Dang, Nam Hoang Tran, Ono, K., Ishimura, K. and Fukui, K
  • Journal Title: J. Biochem Volume: Vol. 153、No. 1 Pages: 93-101
  • DOI: DOI;10.1093/jb/mvs119
  • Peer Reviewed
• [Journal Article] The role of kupffer cells in carbon tetrachloride intoxication in mice (2012)
  • Author(s): Kiso, K., Ueno, S., Fukuda, M., Ichi, I., Kobayashi, K., Sakai, T., Fukui, K., Kojo, S
  • Journal Title: Biol. Pharm. Bull Volume: Vol. 35、No. 6 Pages: 980-983
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/22687543
  • Peer Reviewed
• [Journal Article] Inflammatory disease and cancer with a decrease in Kupffer cell numbers in Nucling-knockout mice (2010)
  • Author(s): Sakai, T., Liu, L., Teng, X. C., Ishimaru, N., Mukai-Sakai, R., Tran, N. H., Kim, S. M., Sano, N., Hayashi, Y., Kaji, R. and Fukui, K
  • Journal Title: Int. J. Cancer Volume: Vol. 126 Pages: 1079-1094
  • DOI: DOI;10.1002/ijc.24789
  • Peer Reviewed
• [Journal Article] NF-kappaB regulates the expression of Nucling, a novel apoptosis regulator, with involvement of proteasome and caspase for its degradation (2010)
  • Author(s): Tran N H, Sakai, T., Kim, S. M. and Fukui, K
  • Journal Title: J Biochem Volume: Vol. 148、No.5 Pages: 573-580
  • DOI: DOI;10.1093/jb/mvq089
  • Peer Reviewed
• [Presentation] 新規NF-・B制御分子ヌクリングは非アルコール性脂肪肝及び糖尿病発症抑制に重要で或る (2012)
  • Author(s): 坂井隆志
  • Organizer: 第16回日本心血管内分泌代謝学会学術総会
  • Place of Presentation: 東京都港区
  • Year and Date: 2012-11-23
• [Presentation] ホモシステイン代謝系における新規NF-・B制御因子ヌクリングの関与 (2012)
  • Author(s): 佐古有季哉
  • Organizer: 日本ビタミン学会第64回大会
  • Place of Presentation: 岐阜市
  • Year and Date: 2012-06-23
• [Presentation] 新規NF-・B制御分子ヌクリングの炎症性病態への関与についての検討 (2012)
  • Author(s): 金善美
  • Organizer: 第53回日本生化学会 中国・四国支部例会
  • Place of Presentation: 岡山市
  • Year and Date: 2012-05-18
• [Presentation] 新規アポトーシス/ NF-κB制御分子ヌクリングの機能解析 (2011)
  • Author(s): 坂井隆志
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 京都市
  • Year and Date: 2011-09-13
• [Presentation] 新規NF-・B制御分子ヌクリングの免疫系における役割の検討 (2011)
  • Author(s): 金善美
  • Organizer: 第52回日本生化学会 中国・四国支部例会
  • Place of Presentation: 広島市
  • Year and Date: 2011-05-13
• [Presentation] Intracellular metabolism of Nucling, a novel apoptosis regulator (2010)
  • Author(s): Sakai T
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-12-08
• [Presentation] Physiological function of a novel NF-・B-regulating molecule, Nucling, in immune system (2010)
  • Author(s): 金善美
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-12-08
• [Presentation] Nucling, a novel apoptosis-associating protein, regulates NF-・B pathway、OzBio2010 (2010)
  • Author(s): Sakai, T.
  • Organizer: the 12th IUBMB, 21st FAOBMB & ComBio2010 Conferences
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 2010-09-27
• [Presentation] Physiological function of a novel NF-・B-regulating molecule, Nucling, in immune system 、OzBio2010 (2010)
  • Author(s): Kim S. M
  • Organizer: the 12th IUBMB, 21stFAOBMB & ComBio2010 Conferences
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 2010-09-27