Investigation of the regulatory mechanisms of cellular energy metabolism by snail

2012 Fiscal Year Final Research Report

• Project/Area Number: 22590287
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: Kagoshima University
• Principal Investigator: HARAGUCHI Misako 鹿児島大学, 大学院・医歯学総合研究科, 准教授 (10244229)
• Co-Investigator(Kenkyū-buntansha): OZAWA Masayuki 鹿児島大学, 大学院・医歯学総合研究科, 教授 (90136854)
• Project Period (FY): 2010 – 2012
• Keywords: Snail / ピルビン酸デヒドロゲナーゼキナーゼ(PDK1) / VLCAD / ATPクエン酸リアーゼ / ATP / グルコース

Research Abstract

Snail expression decreases oxidative phosphorylation and increases glucose dependency in MDCK cells. MDCK/Snail cells exhibited reduced activity of pyruvate dehydrogenase (PDH), which controls pyruvate entry into the TCA cycle, and increased promoter activity and expression of pyruvate dehydrogenase kinase 1 (PDK1), which inhibits PDH. In addition, the activities of TCA cycle enzymes were decreased in MDCK/snail cells. Consequently, MDCK/snail cells exhibited lower O2 consumption and ATP levels than MDCK/neo cells. MDCK/snail cells also showed reduced expression of enzymes essential for glutaminolysis and fatty acid synthesis.

Research Products

• [Journal Article] The transcription factor Snail expressed in cutaneous squamous cell carcinoma induces epithelial-mesenchymal transition and down-regulates COX-2 (2013)
  • Author(s): Shimokawa M, Haraguchi M, Kobayashi W, Higashi Y, Matsushita S, Kawai K, Kanekura T, Ozawa M.
  • Journal Title: Biochem Biophys Res Commun Volume: 430 Pages: 1078-82
  • DOI: DOI:10.1016/j.bbrc.2012.12.035
  • Peer Reviewed
• [Journal Article] The transcription factor Snail enhanced the degradation of E-cadherin and desmoglein 2 in oral squamous cell carcinoma cells (2013)
  • Author(s): Kume K, Haraguchi M, Hijioka H, Ishida T, Miyawaki A, Nakamura N, Ozawa M
  • Journal Title: Biochem Biophys Res Commun Volume: 430 Pages: 889-94
  • DOI: DOI:10.1016/j.bbrc.2012.12.060.
  • Peer Reviewed
• [Journal Article] Snail modulates cell metabolism in MDCK cells (2012)
  • Author(s): Haraguchi M, Indo HP, Iwasaki Y, Iwashita Y, Fukushige T, Majima HJ, Izumo K, Horiuchi M, Kanekura T, Furukawa T, Ozawa M.
  • Journal Title: Biochem Biophys Res Commun Volume: 432 Pages: 618-25
  • DOI: DOI:10.1016/j.bbrc.2013.02.035
  • Peer Reviewed
• [Journal Article] Thymidine phosphorylase enhances reactive oxygen species generation and interleukin-8 expression in human cancer cells (2012)
  • Author(s): Tabata S, Ikeda R, Yamamoto M, Furukawa T, Kuramoto T, Takeda Y, Yamada K, Haraguchi M, Nishioka Y, Sone S, Akiyama S.
  • Journal Title: Oncol Rep Volume: 28 Pages: 895-902
  • DOI: DOI:10.3892/or.2012.1887
  • Peer Reviewed
• [Presentation] Snail による細胞内代謝の新規調節機構 (2012)
  • Author(s): 原口みさ子、小澤政之
  • Organizer: 生化学会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-15
• [Presentation] Snail reduces oxidative phosphorylation in MDCK cells (2012)
  • Author(s): Misako Haraguchi, Masayuki Ozawa
  • Organizer: Keystone Symposia Conference
  • Place of Presentation: Canada Banff
  • Year and Date: 2012-02-14
• [Presentation] Snail による細胞内代謝調節機構 (2011)
  • Author(s): 原口みさ子、小澤政之
  • Organizer: 生化学会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-23