2012 Fiscal Year Final Research Report
Invasion-associated microRNA of small lung adenocarcinoma-A detailed study using laser microdissection
| Project/Area Number |
22590307
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Akita University (2011-2012)
The University of Tokyo (2010) |
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Principal Investigator |
GOTO Akiteru 秋田大学, 大学院・医学系研究科, 教授 (90317090)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 呼吸器・縦隔 / 浸潤・転移 / エピジェネティクス |
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| Research Abstract |
Invasion-associated microRNAs in lung adenocarcinoma were identified by microarray analysis of microRNA. The samples were microdissected from invasive and non-invasive parts of small lung adenocarcinomas. Among 143 microRNAs differentially expressed by more than 2-folds, sequential qRT-PCR study validated five microRNAs (miR-21, miR-107, miR-141, miR-146a, miR-451) as invasion-associated microRNAs, regardless of EGFR gene status. We also identified BTG2 gene as the target of mir-146a. As for the stromal cells in invasive part of lung adenocarcinoma, the combination of immunohistochemistry of smooth-muscle actin and in situ hybridization of mir-21 revealed strong expression of miR-21 in myofibroblasts.
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Author(s)
Shoji K, Murayama T, Mimura I, Wada T, Kume H, Goto A, Ohse T, Tanaka T, Inagi R, van der Hoorn FA, Manabe I, Homma Y, Fukayama M, Sakurai T, Hasegawa T, Aburatani H, Kodama T, Nangaku M.
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Journal Title
DOI
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[Journal Article] High-grade Lung Adenocarcinoma With Fetal Lung-like Morphology: Clinicopathologic, Immunohistochemical, and Molecular Analyses of 17 Cases2013
Author(s)
Morita S, Yoshida A, Goto A, Ota S, Tsuta K, Yokozawa K, Asamura H, Nakajima J, Takai D, Mori M, Oka T, Tamaru J, Itoyama S, Furuta K, Fukayama M, Tsuda H.
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Journal Title
Am J Surg Pathol
Volume: 37(6)
Pages: 924-932
DOI
Peer Reviewed
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[Journal Article] Genome structure-based screening identified epigenetically silenced microRNA associated with invasiveness in non-small-cell lung cancer2012
Author(s)
Watanabe K, Emoto N, Hamano E, Sunohara M, Kawakami M, Kage H, Kitano K, Nakajima J, Goto A, Fukayama M, Nagase T, Yatomi Y, Ohishi N, Takai D.
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Journal Title
Int J Cancer
Volume: 130
Pages: 2580-2590
DOI
Peer Reviewed
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[Journal Article] Pulmonary Venous Invasion, Determined by Chest Computed Tomographic Scan, as a Potential Early Indicator of Zygomycosis Infection: A Case Series2012
Author(s)
Kitagawa H, Watanabe K, Kage H, Inoh S, Goto A, Fukayama M, Nagase T, Ohishi N, Takai D
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Journal Title
J Thorac Imaging
Volume: 27
Pages: W97-99
DOI
Peer Reviewed
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[Journal Article] Quantitative analysis of viral load per haploid genome revealed the different biological features of merkel cell polyomavirus infection in skin tumor2012
Author(s)
Ota, S., Ishikawa, S., Takazawa, Y., Goto, A., Fujii, T., Ohashi, K., Fukayama, M
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Journal Title
PLOS ONE.
Volume: 7
Pages: e39954
DOI
Peer Reviewed
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[Journal Article] Harris CC. Macrophages, nitric oxide and microRNAs are associated with DNA damage response pathway and senescence in inflammatory bowel disease2012
Author(s)
Sohn J, Schetter A, Yfantis H, Ridnour L, Horikawa I., Khan M, RoblesA, Hussain S, Goto, A., Bowman E, Hofseth L, Bartkova J, Bartek J, Wogan G, Wink D
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Journal Title
PLOS ONE
Volume: 7
Pages: e44156
DOI
Peer Reviewed
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[Journal Article] Aberrant expression of tumor suppressors, CADM1 and 4.1B, in invasive lesions of primary breast cancer2012
Author(s)
Takahashi Y, Iwai M, Kawai T, Arakawa A, Ito T, Sakurai-Yageta M, Ito A, Goto A, Saito M, Kasumi F, and Murakami Y.
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Journal Title
Breast Cancer
Volume: 19
Pages: 242-252
Peer Reviewed
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[Journal Article] Aberrations of a cell adhesion molecule CADM4 in renal clear cell carcinoma2012
Author(s)
Nagata M, Sakurai-Yageta M, Yamada D, Goto A, Ito A, Fukuhara H, Kume H, Morikawa T, Fukayama M, Homma Y, and Murakami Y
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Journal Title
Int J Cancer
Volume: 130
Pages: 1329-1337
DOI
Peer Reviewed
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[Journal Article] Involvement of miR-214 and miR-375 in malignant features of non-small-cell lung cancer by down-regulating CADM12012
Author(s)
Ishimura, M., Sakurai-Yageta, M., Maruyama, T., Ando, T., Fukayama,, M., Goto, A., Murakami, Y.
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Journal Title
Journal of Cancer Therapy
Volume: 3
Pages: 379-387
DOI
Peer Reviewed
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[Journal Article] Human papillomavirus infection in lung and esophageal cancers: analysis of 485 Asian cases2011
Author(s)
Goto A, Li CP, Ota S, Niki T, Ohtsuki Y, Kitajima S, Yonezawa S, Koriyama C, Akiba S, Uchima H, Lin YM, Yeh KT, Koh JS, Kim CW, Kwon KY, Nga ME, Fukayama M.
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Journal Title
J Med Virol
Volume: 83
Pages: 1383-1390
DOI
Peer Reviewed
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[Journal Article] CpG island methylation of microRNAs is associated with tumor size and recurrence of non-small-cell lung cancer2011
Author(s)
Kitano K, Watanabe K, Emoto N, Kage H, Hamano E, Nagase T, Sano A, Murakawa T, Nakajima J, Goto A, Fukayama M, Yatomi Y, Ohishi N, Takai D.
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Journal Title
Cancer Sci
Volume: 102
Pages: 2126-2131
DOI
Peer Reviewed
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[Journal Article] (*: Equal contribution): MicroRNA expression and clinical outcomes in patients treated with adjuvant chemotherapy after complete resection of nonsmall cell lung carcinoma2010
Author(s)
Voortman J*, Goto A*, Mendiboure J, Sohn JJ, AJ Schetter, Saito M, Dunant A, Pham TC, Petrini I, Lee A, Khan MA, Hainaut P, Pignon JP, Brambilla E, Popper HH, Filipits M, Harris CC, G Giaccone
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Journal Title
Cancer Res
Volume: 70(21)
Pages: 8288-98
DOI
Peer Reviewed
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