Structure-related activity of tick defensin and antibody monitoring system in wildlife

2012 Fiscal Year Final Research Report

• Project/Area Number: 22590381
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Tohoku University
• Principal Investigator: ISOGAI Emiko 東北大学, 大学院・農学研究科, 教授 (80113570)
• Co-Investigator(Kenkyū-buntansha): ISOGAI Hiroshi 札幌医科大学, 医学研究科, 准教授 (50137436)
• Project Period (FY): 2010 – 2012
• Keywords: マダニ / デフェンシン / 野生動物 / モニタリング / ライム病

Research Abstract

Defensins are major group of antimicrobial peptides and are found widely in vertebrates, invertebrates and plants. Invertebrate defensins have been identified in ticks. In this study, chemically synthesized tick defensins were used to investigate the activity spectrum against bacteria isolated from the midgut. Synthetic tick defensin showedantimicrobial activity against Staphylococcus aureus but not Borrelia garinii and symbiotic Stenotrophomonas maltophila. The results demonstrate that the activity of antimicrobial peptides from tick is important for selection and reservation of specific bacteria such as Lyme disease spirochetes in the midgut. The teriary structure brought more potent activity to S. aureus than primary one in short-time killing. The killing activity is related to the structure of tick defensing. Antibody against tick defensing in wildlife monitoring system can useful to know the risk of tick-borne diseases.

Research Products

• [Journal Article] 磯貝浩、脊椎動物と無脊椎動物の抗菌ペプチド (2012)
  • Author(s): 磯貝恵美子
  • Journal Title: 無菌生物(J. germfree life gnotobiol) Volume: 42(1) Pages: 42-45
• [Journal Article] Tertiary structure-related activity of tick defensin (persulcatusin) in the taiga tick (2011)
  • Author(s): Isogai E, Isogai H, Okumura K, Hori H, Tsuruta H, Kurebayashi Y.
  • Journal Title: Ixodes persulcatus. Exp Appl Acar Volume: 53 Pages: 71-77
  • DOI: Doi:10.1007/s10493-010-9379-3
  • Peer Reviewed
• [Journal Article] マダニデフェンシンの中腸由来細菌に対する抗菌活性 (2011)
  • Author(s): 磯貝恵美子、秋葉敬斉、関翔太、堀初弘、磯貝浩、小林美智子、奥村一彦
  • Journal Title: 無菌生物 Volume: 41(2) Pages: 113-116
• [Journal Article] ライム病感受性に関する遺伝的要因の解析 (2011)
  • Author(s): 磯貝恵美子、米山裕、磯貝浩
  • Journal Title: 遺伝育種研究 Volume: 39(1) Pages: 26-30
  • DOI: DOI:http://dx.doi.org/10.5924/abgri.39.26
• [Presentation] Correlation between levels of antibody against Lyme disease Borrelia and tick defensin in wild deer. (2012)
  • Author(s): Isogai H, Isogai E.
  • Organizer: 3rd International Symposium on Antimicrobial Peptides (Amp2012)
  • Place of Presentation: Lille, France
  • Year and Date: 20120713-15
• [Presentation] 脊椎動物と無脊椎動物の抗菌ペプチド(特別講演) (2012)
  • Author(s): 磯貝恵美子、磯貝浩
  • Organizer: 第45回日本無菌生物ノートバイオロジー学会
  • Place of Presentation: 岐阜
  • Year and Date: 20120112-13
• [Presentation] ライム病ボレリアと宿主(招待講演) (2012)
  • Author(s): 磯貝恵美子、磯貝浩
  • Organizer: 日本ブラキスピラ学会(特別講演)
  • Place of Presentation: 栃木県・自治医科大学
  • Year and Date: 2012-07-28
• [Presentation] Wild deer have antibodies recognizing tick defensin with tertiary structure, Joint Meeting of the 17th International Symposium on Gnotobiology (ISG) and the 34th Congress of Society for Microbial Ecology and Disease (SOMED) (2011)
  • Author(s): Isogai E, Isogai N, Sato K, Yoneyama H, Fukuda T, Deguchi Y, Matsubara K, Murata H, Tuboi T, Haritani M, Miyamoto T, Isogai H
  • Organizer: 第17回国際無菌生物シンポジウム、第34回国際医学微生物生態学会合同会議
  • Place of Presentation: Yokohama
  • Year and Date: 20111120-23
• [Presentation] Homology modeling and tertiary structure-related activity of tick defensin (persulcatusin) in the taiga tick (2011)
  • Author(s): Isogai E, Saito T, Ohmura T, Isogai H, Okumura K, Hori H, Yoneyama H, Tsuruta H, Kurebayashi Y.
  • Organizer: Ixodes persulcatus, IUMS
  • Place of Presentation: Sapporo
  • Year and Date: 20110806-10
• [Book] 犬のライム病、動物の感染症(第3版、共著分担) (2011)
  • Author(s): 磯貝恵美子
• [Book] ライム病、動物病理学各論(共著分担) (2010)
  • Author(s): 磯貝恵美子
  • Total Pages: 464-464
  • Publisher: 文永堂出版