2012 Fiscal Year Final Research Report
Identification of novel toxin genes in CA-MRSA
| Project/Area Number |
22590403
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Bacteriology (including Mycology)
|
|---|
| Research Institution | Juntendo University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Keywords | MRSA / leukocidin / CA-MRSA / HA-MRSA / PVL |
|---|
| Research Abstract |
Community-acquired MRSA (CA-MRSA) has a stronger virulence than Healthcare-assiciated MRSA. Production of Panton-Valentine Leukocidin (PVL) is deemed to be responsible for the strong virulence.However, it was not clear that PVL alone could explain the strong virulence of CA-MRSA. We noticed that CA-MRSA strain MW2 produced another set of leukocidins MW1941/1942. We expressed the toxins in E. coli, purified the toxin proteins and measured their lytic activity against human leukocytes. We observed a synergistic lytic activity of PVL and MW1941/1942 against human leukocytes.
|
