2012 Fiscal Year Final Research Report
Investigation of the defensive mechanisms of HIV-1 Vpu against the antiviral host factor BST-2
| Project/Area Number |
22590428
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
TOKUNAGA Kenzo 国立感染症研究所, 感染病理部, 主任研究官 (50342895)
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| Co-Investigator(Renkei-kenkyūsha) |
TATSUMI Masashi 国立感染症研究所, エイズ研究センター, 室長 (00133629)
FUJITA Hideaki 九州大学, 薬学研究科, 助教 (80291524)
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| Project Period (FY) |
2010 – 2012
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| Keywords | HIV-1 / Vpu / BST-2 / 宿主因子 |
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| Research Abstract |
HIV-1 Vpu enhances virion production by downregulating the antiviral host factor BST-2 from the cell surface. In this study, we attempted to identify host cofactors that play a supporting role in the anti-BST-2 activity of Vpu. We first prepared Vpu-binding proteins by performing immunoprecipitation assay, and identified multiple candidate proteins by proteome analyses. The candidate proteins were cloned into expression plasmids and then co-expressed with Vpu, followed by immunoprecipitation and subcellular localization assays. Knocking-down the gene expression of the candidate proteins that proved to interact with and colocalize with Vpu revealed no effect on Vpu’s anti-BST-2 activity, implying that those proteins might be involved in other functions of Vpu.
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[Journal Article] Characteristics of IFITM, the newly identified IFN-inducible anti-HIV-1 family proteins.2013
Author(s)
Chutiwitoonchai, N., Hiyoshi, M., Hiyoshi-Yoshidomi, Y., Hashimoto, M., Tokunaga, K., and Suzu, S
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Journal Title
Microbes Infect
Volume: 15
Pages: 280-90
Peer Reviewed
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[Journal Article] Identification of SNF2h, a Chromatin-Remodeling Factor, as a Novel Binding Protein of Vpr of Human Immunodeficiency Virus Type 1.2011
Author(s)
Taneichi, D., Iijima, K., Doi, A., Koyama, T., Minemoto, Y., Tokunaga, K., Shimura, M., and Ishizaka, Y
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Journal Title
J. Neuroimmune Pharm
Volume: 6
Pages: 177-87
Peer Reviewed
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[Journal Article] Intrinsic restriction activity by apolipoprotein B mRNA editing enzyme APOBEC1 against the mobility of autonomous retrotransposons.2011
Author(s)
Ikeda, T., Abd El Galil, K., Tokunaga, K., Maeda, K., Sata, T., Sakaguchi, N., Harada, S., Heidmann, T., and Koito, A
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Journal Title
Nucleic Acids Res
Volume: 39
Pages: 5538-5554
Peer Reviewed
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[Journal Article] Epigenetic displacement of HP1 from heterochromatin by HIV-1 Vpr causes premature sister chromatid separation.2011
Author(s)
Shimura, M., Toyoda, Y., Iijima, K., Kinomoto, M, Tokunaga, K., Yoda, K., Yanagida, M., Sata, T., and Ishizaka, Y
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Journal Title
J. Cell Biol
Volume: 194
Pages: 721-35
Peer Reviewed
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[Journal Article] Two N-linked glycosylation sites in V2 and C2 regions of human immunodeficiency virus type 1 CRF01_AE envelope glycoprotein gp120 regulate viral neutralization susceptibility to the human monoclonal antibody specific for CD4 binding domain.2010
Author(s)
Utachee, P., Nakamura, S., Isarangkura-na-ayuthaya, P., Tokunaga, K., Sawanpanyalert, P., Ikuta, K., Auwanit, W., and Kameoka, M
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Journal Title
J. Virol
Volume: 84
Pages: 4311-20
Peer Reviewed
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[Journal Article] Combined analysis of cell growth and apoptosis-regulating proteins in HPVs associated anogenital tumors.2010
Author(s)
Mitsuishi, T., Iwabu, Y., Tokunaga, K., Sata, T., Kaneko, T., Ohara, K., Ohsawa, I., Oda, F., Kato, T., Yamada, Y., Kawana, S., Ozaki, K., Nakatake, M., and Yamada, O
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Journal Title
BMC cancer
Volume: 10
Pages: 118
Peer Reviewed
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[Journal Article] The role of lysine residue at amino acid position 165 of human immunodeficiency virus type 1 CRF01_AE Gag in reducing viral drug susceptibility to protease inhibitors.2010
Author(s)
Kameoka, M, Isarangkura-na-ayuthaya, P., Kameoka, Y., Sapsutthipas, S., Soonthornsata, B., Nakamura, S., Tokunaga, K., Sawanpanyalert, P., Ikuta, K., and Auwanit, W.
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Journal Title
Virology
Volume: 405
Pages: 129-38
Peer Reviewed
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[Journal Article] Differential anti-APOBEC3G activity of HIV-1 Vif proteins derived from different subtypes.2010
Author(s)
Iwabu, Y., Kinomoto, M., Tatsumi, M., Fujita, H., Shimura, M., Tanaka, Y., Ishizaka, Y., Nolan, D., Mallal, S., Sata, T., and Tokunaga, K (corresponding author)
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Journal Title
J. Biol. Chem
Volume: 285
Pages: 35350-35358
Peer Reviewed
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[Presentation] Anti-APOBEC3G activity of HIV-1 Vif protein from elite controllers is attenuated compared to those from untreated chronic progressors or those from individuals with acute infection.2012
Author(s)
T. Kikuchi, Y. Iwabu, A. Kawana- Tachikawa, M. Koga, N. Hosoya, S. Nomura1, Z.L. Brumme, H. Jessen, A. Kelleher, M. Markowitz, F. Pereyra, A. Trocha, B.D. Walker, A. Iwamoto, K. Tokunaga, and T. Miura
Organizer
XIX International AIDS Conference
Place of Presentation
Washington D.C., USA
Year and Date
20120700
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[Presentation] Infectivity of HIV-1 Subtypes Isolated from Ghanaian Patients.2011
Author(s)
Barnor, J., Tatsumi, M., Sata, T., Ampofo, W., Osei-Kwasi, M., Tokunaga, K
Organizer
Patients.WSU Joint International Conference. East London
Place of Presentation
South Africa
Year and Date
20110900
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[Presentation] Targeting of recycling endosomes to the cleavage furrow requires ubiquitylation of membrane proteins.2010
Author(s)
Hideaki Fujita, Yukie Iwabu, Kenzo Tokunaga, Tetsutaro Sata, Keiko Fujimoto, Ryo Tachiyama, Daisuke Ishikawa, Masaki Matsumoto, Keiichi I Nakayama, and Yoshitaka Tanaka
Organizer
Biochemistry and Cell Biology of ESCRTs in Health and Disease (ASBMB Special Meeting)
Place of Presentation
Utah, USA.
Year and Date
20101000
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