Immune regulation through dendritic cell-specific mTOR pathway

2012 Fiscal Year Final Research Report

• Project/Area Number: 22590439
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Immunology
• Research Institution: Kansai Medical University
• Principal Investigator: MATSUDA Satoshi 関西医科大学, 医学部, 准教授 (00286444)
• Co-Investigator(Renkei-kenkyūsha): OHTANI Masashi 関西医科大学, 医学部, 助教 (20383713)
• Project Period (FY): 2010 – 2012
• Keywords: mTOR / 樹状細胞 / IL-10 / Raptor / 免疫制御 / サイトカイン

Research Abstract

Although several reports have suggested the involvement of mTORC1 in development and function of dendritic cells (DCs), its physiological roles remain obscure. By using mTORC1 signal-deficient mice lacking Raptor, an essential component of mTORC1 signal, specifically in DC lineage (referred to here as RaptorDC-/-), we found that impaired mTORC1 signal resulted in the suppression of IL-10 production in intestinal CD11c+CD11b+ DCs. We also found that RaptorDC-/- mice were highly susceptible to dextran sodium sulfate-induced colitis.

Research Products

• [Journal Article] Class I PI3K-mediated Akt and ERK signals play a critical role in FcεRI-induced degranulation in mast cells. (2013)
  • Author(s): Takayama, G., Ohtani, M., Minowa, A., Matsuda, S., and Koyasu, S.
  • Journal Title: Int. Immunol. Volume: vol.25 Pages: 215-220
  • DOI: DOI:10.1093/intimm/dxs105
  • Peer Reviewed
• [Journal Article] Subnuclear cyclin D3 compartments and the coordinated regulation of proliferation and immunoglobulin variable gene repression. (2012)
  • Author(s): Powers, S. E., Mandal, M., Matsuda, S., Miletic, A. V., Cato, M. C., Tanaka, A., Rickert, R. C., Koyasu, S., and Clark, M. R.
  • Journal Title: J. Exp. Med. Volume: vol.209 Pages: 2199-2213
  • DOI: DOI:10.1084/jem.20120800
  • Peer Reviewed
• [Journal Article] mTORC1 in intestinal CD11c+CD11b+ dendritic cells regulates intestinal homeostasis by promoting IL-10 production. (2012)
  • Author(s): Ohtani, M., Hoshii, T., Fujii, H.,Koyasu, S., Hirao, A. and Matsuda, S
  • Journal Title: J. Immunol. Volume: vol.188 Pages: 4736-4740
  • DOI: DOI:10.4049/jimmunol.1200069
  • Peer Reviewed
• [Journal Article] PI3K-Akt-mTORC1-S6K1/2 axis controls Th17 differentiation by regulating Gfi-1 expression and nuclear translocation of RORg. (2012)
  • Author(s): Kurebayashi, Y., Nagai, S., Ikejiri, A., Ohtani, M., Ichiyama, K., Baba, Y., Yamada, T., Egami, S., Hoshii, T., Hirao, A., Matsuda, S., and Koyasu, S.
  • Journal Title: Cell Rep. Volume: vol.1 Pages: 360-373
  • DOI: DOI:10.1016/j.celrep.2012.02.007
  • Peer Reviewed
• [Journal Article] Antigen-independent development of Foxp3+ regulatory T cells suppressing autoantibody production in experimental pemphigus vulgaris. (2011)
  • Author(s): Yokoyama, T., Matsuda, S., Takae, Y., Wada, N., Nishikawa, T., Amagai, M., and Koyasu, S.
  • Journal Title: Int. Immunol. Volume: vol.23 Pages: 365-373
  • DOI: DOI:10.1093/intimm/dxr020
  • Peer Reviewed
• [Presentation] mTOR complex1 is critical for B cell development. (2012)
  • Author(s): Ohtani, M., Fujii, H., Sakai, K., Hoshii, T., Watanabe, T., Koyasu, S., Hirao, A., and Matsuda, S.
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-14
• [Presentation] Role of the mTORC1 signaling pathway in acquired immunity. (2012)
  • Author(s): Matsuda, S., Nagai, S., Hoshii, T., Koyasu, S., Hirao, A., and Ohtani, M.
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-13
• [Presentation] Role of the mTORC1 signaling pathway in T cell function. (2012)
  • Author(s): Matsuda, S., and Ohtani, M.
  • Organizer: 第41回日本免疫学会総会・学術集会
  • Place of Presentation: 神戸
  • Year and Date: 2012-12-05
• [Presentation] Role of the mTORC1 signaling pathway in T cell development. (2011)
  • Author(s): Matsuda, S. and Ohatni, M.
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 幕張
  • Year and Date: 2011-11-29
• [Presentation] Role of mTORC1 in immune function of dendritic cells. (2011)
  • Author(s): Ohatni, M. and Matsuda, S.
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 幕張
  • Year and Date: 2011-11-29
• [Presentation] PI3K-Akt-mTORC1 axis controls Th17 differentiation by regulation of Gfi-1 and RORγt. (2011)
  • Author(s): Nagai, S., Kurebayashi, Y., Baba, Y.,Matsuda, S., and Koyasu, S.
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 幕張
  • Year and Date: 2011-11-28
• [Remarks]
  • URL: http://www3.kmu.ac.jp/bioinfo/