2012 Fiscal Year Final Research Report
Hyperdry human amniotic membrane is useful material for tissue engineering.: Physical, morphological properties, and safety as the new biological material.
| Project/Area Number |
22590498
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied pharmacology
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| Research Institution | University of Toyama |
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Principal Investigator |
OKABE Motonori 富山大学, 大学院・医学薬学研究部, 助教 (60283066)
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| Co-Investigator(Kenkyū-buntansha) |
NIKAIDO Toshio 富山大学, 大学院・医学薬学研究部, 教授 (50180568)
YOSHIA Toshiko 富山大学, 大学院・医学薬学研究部, 准教授 (00171421)
NOMURA Yoshihiro 東京農工大学, 農学部, 准教授 (10228372)
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| Co-Investigator(Renkei-kenkyūsha) |
MIZUTA Syoushi 福井県立大学, 生物資源学, 准教授 (30254246)
HAYASHI Kyouko 富山大学, 大学院・医学薬学研究部, 講師 (60110623)
DOKI Yoshinori 富山大学, 大学院・医学薬学研究部, 講師 (90303221)
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| Project Period (FY) |
2010 – 2012
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| Keywords | ヒト組織利用研究 / ヒト乾燥羊膜 / 海洋性コラーゲン |
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| Research Abstract |
Human amniotic membrane (AM) has been used widely as graft biomaterial for a variety of clinical applications. But, there are some persistent problems related to the preparation, storage, and sterilization. To resolve these problems, we developed hyperdry AM (HD-AM) using far-infrared rays, depression of air, and microwaves and then sterilized by g-ray irradiation. To elucidate the benefit of HD-AM as biological materials, compare with the histological and physical properties of HD-AM with a freeze-dried AM (FD-AM) as typical freeze-dry methods, evaluate the safety of HD-AM in vivo experiment used nude mice, and demonstrate the feasibility of HD-AM transplant in pterygium. HD-AM has kept the morphological structure of epithelium and connective tissues. The water permeability and the sieving coefficient of HD-AM were significantly lower than that of FD-AM. At 18 months after transplanted, single and multilayers of HD-AM in the intraperitoneal cavity was degraded without any infiltrated cells. For clinical treatment, recurrence of pterygium and regrowth of the subconjunctival fibrosis were not observed during the 6-month follow-up periods after the surgery. It was proposed that HD-AM was a safe and effective new biological material for clinical use including treatment for recurrent pterygium.
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[Journal Article] Establishment of immortalized human amniotic mesenchymal stem cells2013
Author(s)
Teng, Z.,Yoshida, T.,Okabe, M.,Toda, A.,Higuchi, O.,Nogami, M.,Yoneda, N.,Zhou, K.,Kyo, S.,Kiyono, T.,Nikaido, T
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Journal Title
Cell Transplant
Volume: 22(2)
Pages: 267-78
DOI
Peer Reviewed
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[Journal Article] Establishment and characterization of immortalized human amniotic epithelial cells2012
Author(s)
Zhou, K.,Koike, C.,Yoshida, T.,Okabe, M.,Fathy, M.,Kyo, S.,Kiyono, T.,Saito, S.,Nikaido, T
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Journal Title
Cellular reprogramming
Volume: 15
Pages: 2012-0021
DOI
Peer Reviewed
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[Journal Article] A role for IL-17 in induction of an inflammation at the fetomaternal interface in preterm labour2010
Author(s)
Ito M., Nakasima A., Hidaka T., Okabe M., Bac ND., Ina S., Yoneda S., Shiozaki A., Sumi S., Tuneyama K., Nikaido T., Saito S
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Journal Title
J Reprod Immunol
Volume: 84
Pages: 75-85
DOI
Peer Reviewed
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[Presentation] The clinical application of the new dried human amniotic membrane.(Hyper-Dry amnion : HD-AM)2012
Author(s)
Okabe M., Yoshida T., Koike C., Hayashi N.,Fujisaka M., Shojaku H., Arai N., Tsuno H.,Kitagawa K., Endo S., Watanabe Y., Noguchi M.,Hayashi A., Saito S., Nikaido T
Organizer
ISSCR The 10th Annual Meeting
Place of Presentation
Yokohama
Year and Date
20120613-16
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