Enhancement of utility of imaging mass spectrometry in pathophysiological examination

2012 Fiscal Year Final Research Report

• Project/Area Number: 22590522
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Kinki University (2011-2012); Hamamatsu University School of Medicine (2010)
• Principal Investigator: ZAIMA Nobuhiro 近畿大学, 農学部, 講師 (40455572)
• Project Period (FY): 2010 – 2012
• Keywords: 質量顕微鏡法 / イメージング / イメージングマススペクトロメトリー / ATGL変異症 / 動脈硬化 / 疾患検査 / 質量分析

Research Abstract

The aim of this study is to enhance utility of imaging mass spectrometry (IMS) as new pathophysiological examination. IMS is a two-dimensional MS technique used to visualize the spatial distribution of biomolecules without extraction, purification, separation, or labeling of biological samples. It is important to estimate general versatility of IMS. In this study, we try to visualize the metabolic variation of diseases, including ATGL mutant disease, atherosclerosis, and abdominal aortic aneurysm. We succeed to find some characteristic changes in disease tissues by using IMS.

Research Products

• [Journal Article] *equal contribution Adventitiavasa vasorum arteriosclerosisin abdominal aortic aneurysm (2013)
  • Author(s): Tanaka, H*., Zaima, N*., Sasaki, T., Hayasaka, T., Goto-Inoue, N., Onoue, K., Ikegami, K., Morita, Y., Yamamoto, N., Mano, Y., Sano, M., Saito, T., Sato, K., Konno, H., Setou, M
  • Journal Title: Plos One Volume: 8
  • DOI: doi:10.1371/journal.pone.0057398
  • Peer Reviewed
• [Journal Article] Imaging mass spectrometry-based histopathologic examination of atherosclerotic lesions (2011)
  • Author(s): Zaima, N., Sasaki, T., Tanaka, H., Cheng, W. X., Onoue, K., Hayasaka, T., Goto-Inoue, N., Enomoto, H., Unno, N., Kuzuya, M., Setou, M
  • Journal Title: Atherosclerosis Volume: 217 Pages: 427-432
  • Peer Reviewed
• [Journal Article] Matrix-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry (2010)
  • Author(s): Zaima, N., Hayasaka, T., Goto-Inoue, N., Setou, M
  • Journal Title: International Journal of molecular sciences Volume: 11 Pages: 5040-5055
  • Peer Reviewed
• [Presentation] IMSによる組織内代謝変動の可視化-食品成分動態解析と病理解析への応用- (2013)
  • Author(s): 財満 信宏
  • Organizer: 日本農芸化学会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2013-03-27
• [Presentation] メタボローム可視化の生命科学への応用 (2012)
  • Author(s): 財満 信宏
  • Organizer: 生命医薬情報学連合大会
  • Place of Presentation: タワーホール船堀(東京)
  • Year and Date: 2012-10-16