2012 Fiscal Year Final Research Report
Generation of recombinant antibody libraries that facilitate highly sensitive monitoring of low molecular weight biomarkers
| Project/Area Number |
22590542
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Kobe Pharmaceutical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OYAMA Hiroyuki 神戸薬科大学, 薬学部, 助教 (80572966)
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| Co-Investigator(Renkei-kenkyūsha) |
KOYAMA Junko 神戸薬科大学, 薬学部, 講師 (60102109)
MORITA Izumi 神戸薬科大学, 薬学部, 助手 (20299085)
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| Project Period (FY) |
2010 – 2012
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| Keywords | バイオマーカー / モニタリング / 抗体 |
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| Research Abstract |
To generate high-performance scFv mutants that are specific to small biomarkers, we constructed a hapten- targeting scFv library based on a single master-frame of which VH and VL subgroups are those frequently used in anti-hapten antibodies. However, this library failed to yield any practical binder, suggesting that repeated trials are necessary to select suitable positions/patterns for mutagenesis. Thus, we decided to use streptavidin (stav) as a new scaffold, and generated a library of stav mutatants, each member of which has random mutations in their loop structures. After several rounds of selection, wefound mutant clones that gained binding abilities to estradiol or cortisol.
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[Journal Article] Toward in vivo imaging of heart disease using a radiolabeledsingle-chain Fv fragment targetingTenascin-C.2011
Author(s)
Kobayashi N., Odaka K., Uehara T., Imanaka-Yoshida K., Kato Y., Oyama H., Tadokoro H., Akizawa H., Tanada S., Hiroe M., Fukumura T., Komuro I., Arano Y., Yoshida T., Irie T.
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Journal Title
Anal. Chem.
Volume: 83(23)
Pages: 9123-9130
DOI
Peer Reviewed
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