2013 Fiscal Year Final Research Report
Hishot display - a new combinatorial display for obtaining target-recognizing peptides
| Project/Area Number |
22590544
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Laboratory medicine
|
|---|
| Research Institution | Kanagawa Cancer Center Research Institute |
|---|
Principal Investigator |
TSUJI Shoutaro 地方独立行政法人神奈川県立病院機構神奈川県立がんセンター(臨床研究所), がん治療学部, 主任研究員 (30285192)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
KAGEYAMA Taihei 地方独立行政法人神奈川県立病院機構神奈川県立がんセンター(臨床研究所), がん治療学部, 特別研究員 (20633473)
YAMASHITA Makiko 地方独立行政法人神奈川県立病院機構神奈 川県立がんセンター(臨床研究所), がん治療学部, 客員研究員 (60397776)
WASHIMI Kota 地方独立行政法人神奈川県立病院機構神奈 川県立がんセンター(臨床研究所), その他 部局, 医師 (30716733)
|
|---|
| Project Period (FY) |
2010-04-01 – 2014-03-31
|
| Keywords | 腫瘍検査学 / 生物・生体工学 / 分子認識 / バイオテクノロジー / 遺伝子工学 |
|---|
| Research Abstract |
Display technologies are procedures used for isolating target-recognizing peptides without using immunized animals. In this study, we describe a new display method, named Hishot display, that uses Escherichia coli and an expression plasmid to isolate target-recognizing peptides. This display method is based on the formation, in bacteria, of complexes between a polyhistidine (His)-tagged peptide including random sequences and the peptide-encoding mRNA including an RNA aptamer against the His-tag. Using this display method and a synthetic library of surrogate single-chain variable fragments consisting of VpreB and Ig heavy-chain variable domains, it was possible to isolate clones that could specifically recognize a particular target. The obtained clones could be purified as soluble protein produced by E. coli. The Hishot display will be a useful method to add to the repertoire of display technologies.
|
-
-
-
-
-
-
-
-
-
[Presentation] 悪性胸膜中皮腫と腸上皮化生におけるマーカー蛋白質の発現の比較2012
Author(s)
Makiko Yamashita, Kota Washimi, Taihei Kageyama, Katsuo Suzuki, Mitsuyo Yoshihara, Tomoyuki Yokose, Yohei Miyagi, Shoutaro Tsuji
Organizer
第71回日本癌学会学術総会
Place of Presentation
ロイトン札幌(北海道)
Year and Date
2012-09-21
-
[Presentation] 悪性胸膜中皮腫に対するモノクローナル抗体の作製2012
Author(s)
Taihei Kageyama, Makiko Yamashita, Katsuo Suzuki, Mitsuyo Yoshihara, Kota Washimi, Tomoyuki Yokose, Yohei Miyagi, Shoutaro Tsuji
Organizer
第71回日本癌学会学術総会
Place of Presentation
ロイトン札幌(北海道)
Year and Date
2012-09-21
-
-
-
-
