2012 Fiscal Year Final Research Report
Establishment of predictors in treatment response of chronic hepatitisC by mutational analysis of internal ribosome entry site within hepatitis C virus
Project/Area Number |
22590751
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Gastroenterology
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Research Institution | Kurume University |
Principal Investigator |
OGATA Kei 久留米大学, 医学部, 助教 (90309766)
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Project Period (FY) |
2010 – 2012
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Keywords | C 型慢性肝炎 / IRES / ペグインターフェロン / リバビリン / 効果予測 |
Research Abstract |
Combination therapy with pegylated interferon and ribavirin (below, PEG/RBV treatment) was performed, and 60 or more cases of Chronic Hepatitis C (below, CH-C), resulting in responders, non-responders and relapsers, were analyzed. An analysis was performed ofthe genetic mutation in the internal ribosome entry site (below, IRES) area of the HCV RNA in each case. In the non-response group, many cases had a history of previous interferon therapy, and in those cases, many cases had no mutations in the IRES domain III (below, d III) area. On the other hand, in the response cases, there was a tendency (P<0.05) for many cases to have mutation in IRES d III. In other words, this indicates that there is a possibility that resistant HCV due to previous antiviral therapy is being selected. In the relapse cases, when an analysis was performed of the IRES base mutation cloned from blood serum at the conclusion of treatment (when negative, by a commercially available HCV RNA qualitative metho), it was found that there was a characteristic mutation in base 119 in the IRES area. In the non-response group and response group for PEG/RBV treatment, when IRES base 119 mutations were compared, a discrepancy was observed between both groups, and furthermore, a correlation was found with HCV core area mutations. In other words, a correlation was indicated between the relationship between HCV IRES base mutations and core mutations, and the treatment results. In the search for thetreatment resistant mechanism as a viral factor, this is believed to be an important finding.
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Research Products
(6 results)
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[Journal Article] The Importance of a Prior Psychiatric Examination in Pegylated Interferon and Ribavirin Combination Treatment for Chronic Hepatitis C.2012
Author(s)
Kuhara K, Ide T, Uchimura N, Kumashiro R, Arinaga T, Miyajima I, Murashima S, Ogata K, Kuwahara R, Fujimoto Y, Sakai K,Ishii K, Morita Y, Shirachi M, Sata M
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Journal Title
Kurume Medical Journal
Volume: Vol.59
Pages: 39-44
Peer Reviewed
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