Identification and functional analysis of the factors that interact with cardiac protective factor HB-EGF

2012 Fiscal Year Final Research Report

• Project/Area Number: 22590817
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Circulatory organs internal medicine
• Research Institution: National Cardiovascular Center Research Institute
• Principal Investigator: YAMAZAKI Satoru 独立行政法人国立循環器病研究センター, 研究所, 室長 (70348796)
• Co-Investigator(Kenkyū-buntansha): KITAKAZE Masafumi 独立行政法人国立循環器病研究センター, 研究開発基盤センター, 部長 (20294069)
• Co-Investigator(Renkei-kenkyūsha): TAKASHIMA Seiji 大阪大学, 医学系研究科, 准教授 (90379272)
• Project Period (FY): 2010 – 2012
• Keywords: 遺伝子 / 核酸 / ゲノム / シグナル伝達 / 循環器・高血圧

Research Abstract

In this research project, we were able to establish in vivo enhancer system using zebrafish, in vivo ischemia systems and purification system of trans-regulatory factors to purpose of analyzing the enhancer in heart failure. Although identification of HB-EGF enhancer left some problems, combining these systems is expected to lead to the development of therapies and new pathogenesis of heart failure in future.

Research Products

• [Journal Article] Dipeptidyl-peptidase IV inhibition improves pathophysiology of heart failure and increases survival rate in pressure-overloaded mice. (2013)
  • Author(s): Takahashi A, Asakura M, Ito S, Min KD, Shindo K, Yan Y, Liao Y, Yamazaki S, Sanada S, Asano Y, Ishibashi-Ueda H, Takashima S, Minamino T, Asanuma H, Mochizuki N, Kitakaze M
  • Journal Title: Am J Physiol Heart Circ Physiol. Volume: 304(10) Pages: H1361-9
  • DOI: doi:10.1152/ajpheart.00454.2012
  • Peer Reviewed
• [Journal Article] Ablation of Rnf213 retards progression of diabetes in the Akita mouse. Biochem Biophys Res Commun. Ablation of Rnf213 retards progression of diabetes in the Akita mouse. (2013)
  • Author(s): Yamazaki S, Kobayashi H, Takashima S, Liu W, Okuda H, Yan J, Fujii Y, Hitomi T, Harada KH, Habu T, Koizumi A
  • Journal Title: Biochem Biophys Res Commun Volume: 432(3) Pages: 519-25
  • DOI: doi:10.1016/j.bbrc.2013.02.015
  • Peer Reviewed
• [Journal Article] H(2) mediates cardioprotection via involvements of K(ATP) channels and permeability transition pores of mitochondria in dogs. (2012)
  • Author(s): Yoshida A, Asanuma H, Sasaki H, Sanada S, Yamazaki S, Asano Y, Shinozaki Y, Mori H, Shimouchi A, Sano M, Asakura M, Minamino T, Takashima S, Sugimachi M, Mochizuki N, Kitakaze M
  • Journal Title: Cardiovasc Drugs Ther. Volume: 26(3) Pages: 217-26
  • DOI: doi:10.1007/s10557-012-6381-5
  • Peer Reviewed
• [Journal Article] Identification of RNF213 as a susceptibility gene for moyamoya disease and its possible role in vascular development. (2011)
  • Author(s): 4.Liu W, Morito D, Takashima S, Mineharu Y, Kobayashi H, Hitomi T, Hashikata H, Matsuura N, Yamazaki S, Toyoda A, Kikuta K, Takagi Y, Harada KH, Fujiyama A, Herzig R, Krischek B, Zou L, Kim JE, Kitakaze M, Miyamoto S, Nagata K, Hashimoto N, Koizumi A
  • Journal Title: PLoS One Volume: 6(7) Pages: e22542
  • DOI: doi:10.1371/journal.pone.0022542
  • Peer Reviewed
• [Journal Article] Isoform-specific intermolecular disulfide bond formation of heterochromatin protein 1 (HP1). (2010)
  • Author(s): 5.Higo S, Asano Y, Kato H, Yamazaki S, Nakano A, Tsukamoto O, Seguchi O, Asai M, Asakura M, Asanuma H, Sanada S, Minamino T, Komuro I, Kitakaze M, Takashima S
  • Journal Title: J Biol Chem. Volume: 285(41) Pages: 31337-47
  • DOI: doi:10.1074/jbc.M110.155788
  • Peer Reviewed
• [Journal Article] AMPK controls the speed of microtubule polymerization and directional cell migration through CLIP-170 phosphorylation. (2010)
  • Author(s): Nakano A, Kato H, Watanabe T, Min KD, Yamazaki S, Asano Y, Seguchi O, Higo S, Shintani Y, Asanuma H, Asakura M, Minamino T, Kaibuchi K, Mochizuki N, Kitakaze M, Takashima S
  • Journal Title: Nat Cell Biol. Volume: 12(6) Pages: 583-90
  • DOI: doi:10.1038/ncb2060
  • Peer Reviewed
• [Journal Article] X-box binding protein 1 regulates brain natriuretic peptide through a novel AP1/CRE-like element in cardiomyocytes. (2010)
  • Author(s): Sawada T, Minamino T, Fu HY, Asai M, Okuda K, Isomura T, Yamazaki S, Asano Y, Okada K, Tsukamoto O, Sanada S, Asanuma H, Asakura M, Takashima S, Kitakaze M, Komuro I
  • Journal Title: J Mol Cell Cardiol. Volume: 48(6) Pages: 1280-9
  • DOI: doi:10.1016/j.yjmcc.2010.02.004
  • Peer Reviewed
• [Journal Article] Identification of genes related to heart failure using global gene expression profiling of human failing myocardium. (2010)
  • Author(s): 8.Min KD, Asakura M, Liao Y, Nakamaru K, Okazaki H, Takahashi T, Fujimoto K, Ito S, Takahashi A, Asanuma H, Yamazaki S, Minamino T, Sanada S, Seguchi O, Nakano A, Ando Y, Otsuka T, Furukawa H, Isomura T, Takashima S, Mochizuki N, Kitakaze M
  • Journal Title: Biochem Biophys Res Commun. Volume: 393(1) Pages: 55-60
  • DOI: doi:10.1016/j.bbrc.2010.01.076
  • Peer Reviewed
• [Presentation] Impact of the Polymorphisms of Renin-Angiotensin System on Prognosis in Hypertrophic Cardiomyopathy in the Era of Cardiac Magnetic Resonance. (2013)
  • Author(s): Akira Funada, Hideaki Kanzaki, Satoru Yamazaki, Takuya Hasegawa, Yasuo Sugano, Takahiro Ohhara , Masanori Asakura, Masaharu Ishihara, Satoshi Yasuda, Wataru Shimizu, Hisao Ogawa, Masafumi Kitakaze, Toshihisa Anzai
  • Organizer: 第77回日本循環器学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 20130315-17
• [Presentation] Regulation of mysterin, a moyamoya disease-associated protein, by de-ubiquitinating enzyme. (2012)
  • Author(s): 2.Yuri Kotani, Daisuke Morito, Satoru Yamazaki, Shun-ichiro Iemura, Toru Natsume, Seiji Takashima, Kazuhiro Nagata
  • Organizer: EMBO/EMBL Symposium "Quality Control-From Molecules to Organelles-"
  • Place of Presentation: Heiderberg(Germany)
  • Year and Date: 20120919-22
• [Presentation] Structure and Function of AAA+/ubiquitin ligase Mysterin/RNF213. (2012)
  • Author(s): Daisuke Morito, Yuri Kotani, Kouki Nishikawa, Satoru Yamazaki, Shun-ichiro Iemura, Toru Natsume, Seiji Takashima, Akio Koizumi, Yoshinori Fujimori, Kazuhiro Nagata
  • Organizer: FASEB summer research conferences "Quality Life through Research
  • Place of Presentation: Saxtons River(USA)
  • Year and Date: 20120729-0803
• [Presentation] ゼブラフィッシュモデルによるもやもや病感受性遺伝子mysterinの機能解析 (2012)
  • Author(s): 小林 果、山崎 悟、高島 成二、人見 敏明、劉 万洋、原田 浩二、小泉 昭夫
  • Organizer: 第82回日本衛生学会学術総会
  • Place of Presentation: 京都
  • Year and Date: 20120324-26
• [Presentation] Dipeptidyl-peptidase IV Inhibitor Improved Cardiac Function and Survival in Mice with Pressure Overload Heart Failure. (2012)
  • Author(s): Ayako Takahashi, Masanori Asakura, Shin Ito, Min Kyung-Duk, Kazuhiro Shindo , Yin Yan, Yulin Liao, Satoru Yamazaki, Shoji Sanada, Yoshihiro Asano, H atsue Ishibashi-Ueda, Seiji Takashima, Tetsuo Minamino, Hiroshi Asanuma, Naoki Mochizuki, Masafumi Kitakaze
  • Organizer: 第76回日本循環器学会学術集会
  • Place of Presentation: 福岡
  • Year and Date: 20120316-18
• [Presentation] Structure and function of novel AAA+/Ubiquitin ligase mysterin. (2011)
  • Author(s): Daisuke Morito, Kouki Nishikawa, Wangyang Liu, Satoru Yamazaki, Toshiaki Hitomi, Hatasu Kobayashi, Norio Matsuura, Susumu Miyamoto, Seiji Takashima, Nobuo Hashimoto, Yoshinori Fujiyoshi, Akio Koizumi, Kazuhiro Nagata
  • Organizer: Cold Spring Harbor Asia Conference, "Protein Homeostasis in Health & Disease"
  • Place of Presentation: Suzhou (China)
  • Year and Date: 20110926-30
• [Presentation] Effect of Vildagliptin on the Improvement of Survival in Murine Pressure-Overload Heart Failure Model. (2010)
  • Author(s): Ayako Takahashi, Masanori Asakura, Min Kyung-Duk, Shin Ito, Tetsuo Minamino, Yin Yan, Satoru Yamazaki, Shoji Sanada, Hiroshi Asanuma, Yulin Liao, Seiji Takashima, Naoki Mochizuki, Masafumi Kitakaze
  • Organizer: American Heart Association 83rd Scientific Sessions
  • Place of Presentation: Chicago (USA)
  • Year and Date: 20101113-17
• [Presentation] Inhalation of Hydrogen Gas Reduced Infarct Size Following Ischemia and Reperfusion via Mitochondrial KATP Channels in Canine Hearts. (2010)
  • Author(s): Akemi Yoshida, Hiroshi Asanuma, Hideyuki Sasaki, Shoji Sanada, Satoru Yamazaki, Seiji Takashima, Tetsuo. Minamino, Masanori Asakura, Masafumi Kitakaze
  • Organizer: European Society of Cardiology Congress 2010
  • Place of Presentation: Stockholm (Sweden)
  • Year and Date: 20100828-0901
• [Presentation] 新規巨大ATPアーゼ/ユビキチンリガーゼMysterinは血管新生を制御し、モヤモヤ病(ウイリス動脈輪閉塞症)に関与する (2010)
  • Author(s): 森戸 大介、劉 万洋、山崎 悟、人見 敏明、小林 果、松浦 範夫、箸方 宏州、原田 浩二、高島 成二、宮本 享、橋本 信夫、小泉 昭夫、永田 和宏
  • Organizer: 第62回日本細胞生物会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 20100519-21