2012 Fiscal Year Final Research Report
siRNA for the treatment of airway remodeling
| Project/Area Number |
22590859
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Mie University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TAGUCHI Osamu 三重大学, 医学系研究科, 准教授 (00293770)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 肺線維症 / siRNA / RNAi / TGF-β / リモデリング |
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| Research Abstract |
SiRNA has been the focus of attention for its potential application for the development of new drugs.To evaluate the effect of intratracheal administration of siRNAs in murine disease models. Firstly, bleomycin was used for induction of lung fibrosis. After confirming the effectiveness of siRNA effect in vitro, siRNAs for several targets including TGF-beta1 were intratracheally administered by inhalation. Mice treated with scrambled siRNA or vehicle were used as controls. We generated a novel model of human TGF-beta1 transgenic mouse that spontaneously develops pulmonary fibrosis. Intrapulmonary delivery of aerosolized siRNAs of TGF-beta1 significantly inhibited bleomycin-induced pulmonary fibrosis. In addition, aerosolized siRNAs against human TGF-beta1 also inhibited spontaneous pulmonary fibrosis.These results suggest that intratracheal instillation of RNAi agents may be useful for the therapy of airway remodeling.
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Research Products
(10 results)
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[Journal Article] Efficacy of a novel class of RNA interference therapeutic agents2012
Author(s)
Hamasaki T, Suzuki H, Shirohzu H, Matsumoto T, D'Alessandro-Gabazza CN,Gil-Bernabe P, Boveda-Ruiz D, Naito M, Kobayashi T, Toda M, Mizutani T, TaguchiO, Morser J, Eguchi Y, Kuroda M, Ochiya T, Hayashi H, Gabazza EC, Ohgi T
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Journal Title
PLoS One
Volume: 7
Pages: e42655
DOI
Peer Reviewed
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[Journal Article] Correlation between circulating fibrocytes, and activity andprogression of interstitial lung diseases2012
Author(s)
Fujiwara A, Kobayashi H, Masuya M, Maruyama M, Nakamura S, Ibata H, FujimotoH, Ohnishi M, Urawa M, Naito M, Takagi T, Kobayashi T, Gabazza EC, Takei Y,Taguchi O
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Journal Title
Respirology
Volume: 17
Pages: 693-8
DOI
Peer Reviewed
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[Journal Article] Role of thrombin-activatablefibrinolysis inhibitor in allergic bronchial asthma2012
Author(s)
Fujiwara A, Taguchi O, Takagi T, D'Alessandro-Gabazza CN, Boveda-Ruiz D, Toda M, Yasukawa A, Matsushima Y, Miyake Y, Kobayashi H, Kobayashi T, Gil-Bernabe P,Naito M, Yoshida M, Morser J, Takei Y, Gabazza EC.
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Journal Title
Lung
Volume: 190
Pages: 189-98
Peer Reviewed
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[Journal Article] Development and preclinical efficacy of novel transforming growthfactor-β1 short interfering RNAs for pulmonary fibrosis2012
Author(s)
D'Alessandro-Gabazza CN, Kobayashi T, Boveda-Ruiz D, Takagi T, Toda M,Gil-Bernabe P, Miyake Y, Yasukawa A, Matsuda Y, Suzuki N, Saito H, Yano Y, Fukuda A, Hasegawa T, Toyobuku H, Rennard SI, Wagner PD, Morser J, Takei Y, Taguchi O, Gabazza EC
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Journal Title
Am J Respir Cell MolBiol
Volume: 46
Pages: 397-406
Peer Reviewed
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[Journal Article] Inhibition of allergicbronchial asthma by thrombomodulin is mediated by dendritic cells2011
Author(s)
Takagi T, Taguchi O, Toda M, Ruiz DB, Bernabe PG, D'Alessandro-Gabazza CN,Miyake Y, Kobayashi T, Aoki S, Chiba F, Yano Y, Conway EM, Munesue S, Yamamoto Y,Yamamoto H, Suzuki K, Takei Y, Morser J, Gabazza EC
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Journal Title
Am J RespirCrit Care Med
Volume: 183
Pages: 31-42
Peer Reviewed
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[Journal Article] Efficacy of 23-valent pneumococcal vaccine in preventing pneumonia and improving survival in nursing home residents: double blind2010
Author(s)
Maruyama T, Taguchi O, Niederman MS, Morser J, Kobayashi H, Kobayashi T,D'Alessandro-Gabazza C, Nakayama S, Nishikubo K, Noguchi T, Takei Y, Gabazza EC.
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Journal Title
randomised and placebocontrolled trial. BMJ
Volume: 340
Pages: c1004
DOI
Peer Reviewed
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