Study on interaction of intracellular signal in renal tubularinterstitial fibrosis

2012 Fiscal Year Final Research Report

• Project/Area Number: 22590896
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Wakayama Medical University
• Principal Investigator: MORIMOTO Yoshifumi 和歌山県立医科大学, 医学部, 博士研究員 (90191048)
• Co-Investigator(Kenkyū-buntansha): MURAGAKI Yasuteru 和歌山県立医科大学, 医学部, 教授 (40190904)
• Project Period (FY): 2010 – 2012
• Keywords: 腎臓 / 間質線維化 / Trps1 / EMT / MET

Research Abstract

To study the interaction between Bmp7 and TGF-β intracellular signaling in renal tubular interstitial fibrosis, we used a mouse fibrosis model by unilateral ureteral obstruction (UUO). We compared the degree of renal tubular intestinal fibrosis caused by UUO using wild -type (WT) and Trps1 heterozygous KO (HT) mice. HT mice had more severe fibrosis than WT mice. From this experiment, we concluded that Trps1 functions downstream of Bmp7 and suppresses fibrosis by inhibiting Arkadia expression, suppressing TGF-β signaling.

Research Products

• [Journal Article] Snail protein expression as a hallmark of gastric carcinoma in biopsy samples (2012)
  • Author(s): Tanishima H, Gui T, Sun Y, Shimokado A, Ozaki T, Muragaki Y
  • Journal Title: ISRN Pathology, 2012,Article ID 132472 Pages: 7
  • Peer Reviewed
• [Journal Article] Fibulin-5 protein is reduced in the lung of patients with spontaneous pneumothorax who are under 25 years old (2012)
  • Author(s): Hirai Y, Muragaki Y, Itoh S, Oikawa K, Juri M, Kondo T, Okamura Y
  • Journal Title: Ann Thorac Cardiovasc Surg Volume: 18(3) Pages: 200-5
  • Peer Reviewed
• [Journal Article] A novel oncogenic pathway by TLS-CHOP involving repression of MDA-7/IL-24 expression (2012)
  • Author(s): Oikawa K, Tanaka M, Itoh S, Takanashi M, Ozaki T, Muragaki Y,Kuroda M.
  • Journal Title: Br J Cancer Volume: 106(12) Pages: 1976-9
  • Peer Reviewed
• [Journal Article] Trps1 is necessary for normal temporomandibular joint development (2012)
  • Author(s): Michikami I, Fukushi T, Honma S, Yoshioka S, Itoh S, Muragaki Y, Kurisu K, Ooshima T, Wakisaka S, Abe M.
  • Journal Title: Cell Tissue Res Volume: 348(1) Pages: 131-40
  • Peer Reviewed
• [Journal Article] MicroRNAs that target Ca2+ transporters are involved in vascular smooth muscle cell calcification (2012)
  • Author(s): Gui T, Zhou G, Sun Y, Shimokado A, Itoh S, Oikawa K, Muragaki Y
  • Journal Title: Lab Invest Volume: 92 Pages: 1250-1259
  • Peer Reviewed
• [Journal Article] The Roles of Mitogen-Activated Protein Kinase Pathways in TGF-β-Induced Epithelial-Mesenchymal Transition (2012)
  • Author(s): Gui T, Sun Y, Shimokado A, MuragakiY.
  • Journal Title: Signal Transduct.
  • Peer Reviewed
• [Journal Article] Diverse roles of macrophages in atherosclerosis: from inflammatory biology to biomarker discovery (2012)
  • Author(s): Gui T, Shimokado A, Sun Y, Akasaka T, Muragaki Y
  • Journal Title: Mediators Inflamm
• [Journal Article] The Roles of Mitogen-Activated Protein Kinase Pathways in TGF-β-Induced Epithelial-Mesenchymal Transition (2012)
  • Author(s): Gui T, Sun Y, Shimokado A, Muragaki Y
  • Journal Title: J Signal Transduct
  • Peer Reviewed
• [Journal Article] The function of TRPS1 in the development and differentiation of bone, kidney, and hair follicles. (2011)
  • Author(s): Gai Z, Gui T, Muragaki Y
  • Journal Title: Histol Histopathol Volume: 26(7) Pages: 915-21
  • Peer Reviewed
• [Journal Article] Increased type I and V collagen expression in uterine leiomyomas during the menstrual cycle (2011)
  • Author(s): Iwahashi M, Muragaki Y
  • Journal Title: Fertil Steril Volume: 95(6) Pages: 2137-9
  • Peer Reviewed
• [Journal Article] Loss of Smad3 gives rise to poor soft callus formation and accelerates early fracture healing (2011)
  • Author(s): Kawakatsu M, Kanno S, Gui T, Gai Z, Itoh S, Tanishima H, Oikawa K, Muragaki Y
  • Journal Title: Exp Mol Pathol Volume: 90(1) Pages: 107-15
  • Peer Reviewed
• [Journal Article] SNAIL induces epithelial-to-mesenchymal transition in a human pancreatic cancer cell line (BxPC3) and promotes distant metastasis and invasiveness in vivo (2010)
  • Author(s): Nishioka R, Itoh S, Gui T, Gai Z, Oikawa K, Kawai M, Tani M, Yamaue H, Muragaki Y.
  • Journal Title: Exp. Mol. Pathol Volume: 89 Pages: 149-157
  • Peer Reviewed
• [Journal Article] Trps1 haploinsufficiency promotes renal fibrosis by increasing Arkadia expression (2010)
  • Author(s): Gai Z, Zhou G, Gui T, Itoh S, Oikawa K, Uetani K, and Muragaki Y.
  • Journal Title: J. Am. Soc. Nephrol Volume: 21 Pages: 1468-1476
  • Peer Reviewed
• [Presentation] Masahiko Kuroda , Yasuteru Muragaki.Wapl regulates HP1 expression and levels of various histone H3 tail modifications (2012)
  • Author(s): Kosuke Oikawa, Masakatsu Takanashi, Mayako Niwa, Yujing Sun, Ting Gui, Aiko Shimokado, Shunji Itoh , Takashi Ozaki, Masahiko Kuroda , Yasuteru Muragaki
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 福岡市
  • Year and Date: 20121200
• [Presentation] TLS-CHOP represses expression of the antitumor cytokine MDA-7/IL24 in myxoid liposarcoma (2012)
  • Author(s): Kosuke Oikawa, Masakatsu Takanashi,Masahiko Kuroda, Yasuteru Muragaki.
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: 札幌
  • Year and Date: 20120900
• [Presentation] 早期胃癌術後 13 年目に播種性骨髄癌症を発症した一剖検例 (2012)
  • Author(s): 下角あい子、松谷泰好、井畑裕三子、尾崎敬、東克彦、村垣泰光
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 東京
  • Year and Date: 20120400
• [Presentation] バーチャルスライドを用いたルーチン病理標本の遠隔診断体制の構築 (2012)
  • Author(s): 森一郎、長村義之、尾崎敬、村垣泰光、井畑孝敏、上田浩
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 東京
  • Year and Date: 20120400
• [Presentation] MicroRNAs are involved in phosphate and calcium-induced vascular smooth muscle cell calcification (2012)
  • Author(s): 桂〓、孫玉静、下角あい子、伊藤俊治、及川恒輔、尾崎敬、村垣泰光
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 東京
  • Year and Date: 20120400
• [Presentation] Gdf5/Trps1 は Prg4 発現を制御し指節骨骨端部形成を調節する (2012)
  • Author(s): 伊藤俊治、孫玉静、桂〓、下角あい子、川勝基久、尾崎敬、及川恒輔、村垣泰光
  • Organizer: 第25回軟骨代謝学会
  • Place of Presentation: 名古屋市
  • Year and Date: 20120300
• [Presentation] Expression of PRG4 variant C specifically functions in tumor formation and development of myxoid liposarcoma (2011)
  • Author(s): Yujing Sun, Kosuke Oikawa, Shunji Itoh, Ting Gui, Aiko Shimokado, Takashi Ozaki, and Yasuteru Muragaki
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20111200
• [Presentation] A novel oncogenic mechanism of the chimeric oncoprotein TLS-CHOP mediated by a tumor suppressor protein MDA-7/IL24 (2011)
  • Author(s): Kosuke Oikawa, Masami Tanaka, Shunji Itoh, Yujing Sun, Ting Gui, Aiko Shimokado, Takashi Ozaki, Masakatsu Takanashi, Masahiko Kuroda, and Yasuteru Muragaki
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20111200
• [Presentation] 粘液型脂肪肉腫における分子治療の可能性を探る (2011)
  • Author(s): 及川恒輔、村垣泰光
  • Organizer: 日本バイオセラピィ学会学術集会総会
  • Place of Presentation: 和歌山
  • Year and Date: 20111200
• [Presentation] Analysis of the novel molecular function of the cohesin-associated protein Wapl (2011)
  • Author(s): Kosuke Oikawa, Masakatsu Takanashi, Masahiko Kuroda,Yasuteru Muragaki.
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 20111000
• [Presentation] カルシウム輸送体をターゲットにするマイクロRNAはリンで誘導される血管平滑筋細胞の石灰化に関与している (2011)
  • Author(s): 桂〓、村垣泰光
  • Organizer: 第43回日本結合組織学会学術大会
  • Place of Presentation: 別府
  • Year and Date: 20110600
• [Presentation] Vascular calcification is suppressed by theinhibition of microRNAs targeting calcium channels (2011)
  • Author(s): 桂〓、村垣泰光
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 横浜
  • Year and Date: 20110400
• [Presentation] Gdf5/Trps1 signaling regulates Prg4 expression in the superficial cells of the articular cartilage. (2011)
  • Author(s): Shunji Itoh, Yujing Sun, Ting Gui, AikoShimokado, Motohisa Kawakatsu, Kosuke Oikawa, Takashi Ozaki, and Yasuteru Muragaki.
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20110200
• [Presentation] MicroRNAs induced by high concentration of phosphate and calcium are involved in vascular smooth muscle cell calcification (2011)
  • Author(s): Gui T, Muragaki Y
  • Organizer: American Society of Nephrology
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 20110000
• [Presentation] Renal interstitial fibrosis is promoted through an elevated amount of Arkadia in heterozygous loss of Trps1 (2010)
  • Author(s): 別府 Gai Z, Gui T, Itoh S, Oikawa K, Uetani K, Muragaki Y
  • Organizer: 第53回日本腎臓学会学術総会
  • Place of Presentation: 神戸
  • Year and Date: 20100600