2012 Fiscal Year Final Research Report
Development of regenerative medicine for spinal cord injury in HTLV-1 associated myelopathy
| Project/Area Number |
22590943
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | St. Marianna University School of Medicine |
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Principal Investigator |
YAMANO Yoshihisa 聖マリアンナ医科大学, 医学(系)研究科(研究院), 准教授 (80445882)
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| Co-Investigator(Kenkyū-buntansha) |
TOMARU Utano 北海道大学, 大学院・医学研究科, 准教授 (20360901)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 神経病態免疫学 / HAMの脊髄再生 |
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| Research Abstract |
We studied somatic stem cells in order to forward the development of regenerative medicine for spinal cord injury in Human T-Lymphotropic Virus Type 1 (HTLV-1) associated myelopathy (HAM). We investigated the infectiousness of HTLV-1 to somatic stem cells and used an animal model for HAM to analyze the regenerative capacity of somatic stem cells as a treatment option. We transplanted adipose derived somatic stem cells (ASCs) from HTLV-1 infected GFP rats into GFP-negative non-infected rats, and no infection was observed. However, when we analyzed the infectiousness to human-derived ASCs in vitro, we clearly observed infection, indicating that there is a risk that ASCs could become infected with HTLV-1. We also attempted to create an animal model of early-onset HAM using HTLV-1 infected rats by challenging the infection every 2 weeks; however, no acceleratory effect was observed. Finally, we analyzed the major pathogenic processes leading to spinal cord lesions in HAM, and we demonstrated that overproduction of CXCL10 from astrocytes plays a major role in HAM pathogenesis.
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