Generation of tumor-bearing humanized mice that express human cytokine

2012 Fiscal Year Final Research Report

• Project/Area Number: 22591027
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hematology
• Research Institution: National Hospital Organization Nagoya Medical Center
• Principal Investigator: SAITO Toshiki 独立行政法人国立病院機構(名古屋医療センター臨床研究センター), 名古屋医療センター臨床研究センター, 室長 (50557238)
• Research Collaborator: KUMAGAI Katsuyoshi 東京医科大学, 医学部, 助教 (20567911) ; HIRANO Naoto トロント大学, 免疫学, 准教授
• Project Period (FY): 2010 – 2012
• Keywords: 癌 / 動物 / 免疫学

Research Abstract

First, we obtained fertilized eggs efficiently by means of in vitro fertilization (IVF); then, we attempted to generate CAG-EGFP Tg mice on an NOD/SCID background, finding that delayed timing of the microinjection after the IVF improved the time to development of the two-cell-stage embryos and the obtainment of newborns. We successfully generated Tg mice and confirmed the germ-line transmission in the offspring. In conclusion, we established a novel system for directly generating transgenic mice on an NOD/SCID background.

Research Products

• [Journal Article] The LRF transcription factor regulates mature B cell development and the germinal center response in mice. (2011)
  • Author(s): Sakurai N, Maeda M, Lee SU, Ishikawa Y, Li M, Williams JC, Wang L, Su L, Suzuki M, Saito TI, Chiba S, Casola S, Yagita H, Teruya-Feldstein J, Tsuzuki S, Bhatia R, Maeda T.
  • Journal Title: J Clin Invest. Volume: 121(7) Pages: 2583-98
  • DOI: doi:10.1172/JCI45682
  • Peer Reviewed
• [Journal Article] In vivo imaging of Treg cells providing immune privilege to the haematopoietic stem-cell niche. (2011)
  • Author(s): Fujisaki J, Wu J, Carlson AL, Silberstein L, Putheti P, Larocca R, Gao W, Saito TI, Lo Celso C, Tsuyuzaki H, Sato T, Cote D, Sykes M, Strom TB, Scadden DT, Lin CP
  • Journal Title: Nature Volume: 474 Pages: 216-219
  • DOI: doi:10.1038/nature10160
  • Peer Reviewed
• [Journal Article] Generation of Transgenic Mice on an NOD/SCID Background Using the Conventional Microinjection Technique. (2011)
  • Author(s): Saito TI, Kumagai K, Kubota N, Sasako T, Takizawa R, Sudo K, Kurokawa M, Kadowaki T.
  • Journal Title: Biol Reprod Volume: 84(4) Pages: 682-8
  • DOI: doi:10.1095/biolreprod.110.087106
  • Peer Reviewed