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2012 Fiscal Year Final Research Report

Exploration of a novel role for lidocaine as a wide range spectrum anti-infection drug

Research Project

  • PDF
Project/Area Number 22591112
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Infectious disease medicine
Research InstitutionTokyo Women's Medical University

Principal Investigator

TAKAKUWA Yuichi  東京女子医科大学, 医学部, 教授 (40113740)

Co-Investigator(Kenkyū-buntansha) KOSHINO Ichiro  東京女子医科大学, 医学部, 助教 (80328377)
MANNO Sumie  東京女子医科大学, 医学部, 講師 (10101205)
TANAKA Shotaro  東京女子医科大学, 医学部, 助教 (90380667)
ARASHIKI Nobuto  東京女子医科大学, 医学部, 助教 (80569658)
Project Period (FY) 2010 – 2012
Keywords脂質ラフト / リドカイン / Plasmodium falciparum / Feline immunodeficiency virus / 抗感染症薬
Research Abstract

Plasmodium falciparum, a causative agent of malaria, and feline immunodeficiency virus were used as models for parasitic and viral infection, respectively. Lidocaine treatment disrupted lipid rafts in respective host cells and concomitantly inhibited the parasite invasion and viral entry. Detailed study revealed that malaria parasites activateraft-mediated signal transduction in host erythrocytes to phosphorylate dematin, a membrane skeletal protein. Phosphorylation of dematin results in a transient loosening of membrane skeletal meshwork, allowing the parasite invasion. Lidocain was shown to inhibit the parasite invasion by preventing membrane skeletal loosening via disruption of lipid rafts

  • Research Products

    (13 results)

All 2012 Other

All Journal Article (1 results) Presentation (12 results)

  • [Journal Article] Identification of a novel role for dematin in regulating red cell membrane function by modulating spectrin-actin interaction2012

    • Author(s)
      Koshino, I., Mohandas, N., andTakakuwa, Y.
    • Journal Title

      J. Biol. Chem

      Volume: 287 Pages: 35244-35250

  • [Presentation] 局所麻酔薬リドカインによる抗ウイルス効果の検討

    • Author(s)
      越野一朗、 高桑雄一
    • Organizer
      日本膜学会第35 年会
  • [Presentation] Possibleinvolvement of ATP derived from infected erythrocytes in invasion by Plasmodium falciparum

    • Author(s)
      野 一 朗 、 高 桑 雄 一
    • Organizer
      第85回日本生化学会大会
  • [Presentation] マラリア原虫の赤血球侵入に利用される GPCR アゴニストに関する検討

    • Author(s)
      越野一朗、 高桑雄一
    • Organizer
      日本膜学会第34 年会
  • [Presentation] Identification of the GPCR-agonist utilized by P. falciparum

    • Author(s)
      越野一朗、 高桑雄一
    • Organizer
      第84回日本生化学会大会
  • [Presentation] Identification of a novel role for dematin in regulating red cell membrane mechanical function by PKA phosphorylation-dependent modulation of spectrin-actin interaction

    • Author(s)
      Koshino, I, Mohandas, N., and Takakuwa Y
    • Organizer
      Red Cell Club Annual Meeting 2011
  • [Presentation] Erythrocyte raft-dependent signal transduction pathway involved in Plasmodium falciparum invasion of erythrocytes

    • Author(s)
      Koshino, I. and Takakuwa, Y
    • Organizer
      International Union of Microbiological Societies Congress 2011
  • [Presentation] マラリア原虫が赤血球侵入に利用する情報伝達経路の解明

    • Author(s)
      越野一朗、 高桑雄一
    • Organizer
      日本膜学会第33 年会
  • [Presentation] lever Mechanism for malaria parasite invasion of human erythrocytes: Innocent participation of the erythrocyte raft-dependent signal transduction pathway

    • Author(s)
      Koshino, I. and Takakuwa, Y.
    • Organizer
      3rd Indo-Japanese Infectious Disease Symposium
  • [Presentation] マラリア原虫の侵入における赤血球膜骨格蛋白質デマチンの役割

    • Author(s)
      越野一朗、 高桑雄一
    • Organizer
      第83回日本生化学会大会
  • [Presentation] 脂質ラフトを舞台とした熱帯熱マラリア原虫の赤血球侵入機構とその防御

    • Author(s)
      高桑雄一
    • Organizer
      日本血液学会
  • [Presentation] デマチンの PKAリン酸化による赤血球膜骨格機能の調節

    • Author(s)
      越野一朗、高桑雄一
    • Organizer
      日本膜学会第32 年会
  • [Presentation] Regulation of erythrocyte membrane stability and malaria parasite invasion by lipid raft-dependent signal transduction

    • Author(s)
      Koshino, I. and Takakuwa, Y.
    • Organizer
      The 13th International Membrane Research Forum, The 6^(th) iCeMS International Symposium

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Published: 2014-08-29  

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