Quality assessment of cryopreserved-mesenchymal stem cells derived from umbilical cord blood

2012 Fiscal Year Final Research Report

• Project/Area Number: 22591150
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Tohoku University
• Principal Investigator: MINEGISHI Masayoshi 東北大学, 病院, 准教授 (20211592)
• Co-Investigator(Kenkyū-buntansha): TSUCHIYA Sigeru 東北大学, 大学院・医学系研究科, 教授 (30124605) ; ONUMA Masaei 東北大学, 病院, 医員 (70436111)
• Project Period (FY): 2010 – 2012
• Keywords: 間葉系幹細胞 / 臍帯血 / 凍結保存

Research Abstract

In this study, we isolated 5 clones of mesenchymal stem cells from human umbilical cord blood. Four clones yielded MSC with more than 1,000-fold expansion capacity after cryopreservation as well as before cryopreservation. Chromosomal analysis showed an abnormal karyotype in one clone after freezing. Further studies are needed to investigate the risk of prolonged culture periods and cryopreservation for the safety of MSC cell therapy.

Research Products

• [Journal Article] Development of a multi-step leukemogenesis model of MLL-rearranged leukemia using humanized mice (2012)
  • Author(s): Moriya K, Suzuki M, Watanabe Y, Takahashi T, Aoki Y, Uchiyama T, Kumaki S, Sasahara Y, Minegishi M, Kure S, Tsuchiya S, Sugamura K, and Ishii N.
  • Journal Title: PLoS ONE Volume: 7(6) Pages: e37892
  • DOI: doi:10.1371/journal.pone.0037892
• [Journal Article] Successful cord blood transplantation with reduced-intensity conditioning for childhood cerebral X-linked adrenoleukodystrophy at advanced and early stages (2012)
  • Author(s): Niizuma H, Uematsu M, Sakamoto O, Uchiyama T, Horino S, Onuma M, Matsuhashi T, Rikiishi T, Sasahara Y, Minegishi M, Tsuchiya S.
  • Journal Title: Pediatr Transplant Volume: 16(2) Pages: E63-70
  • DOI: doi:10.1111/j.1399-3046.2011.01539.x.
• [Journal Article] Quality assessment of umbilical cord blood units at the time of transplantation (2011)
  • Author(s): Kudo Y, Minegishi M, Seki O, Takahashi H, Suzuki A, Narita A, Sato Y, Abe M, Ishioka N, Harigae H, Tsuchiya S.
  • Journal Title: Int J Hematol Volume: 93(5) Pages: 645-51
  • DOI: doi:10.1007/s12185-011-0830-y.