2012 Fiscal Year Final Research Report
Development of new gene therapies for epidermolysis bullosa
| Project/Area Number |
22591215
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Hokkaido University |
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Principal Investigator |
OTA Mitsuhito 北海道大学, 大学院・医学研究科, 非常勤講 師 (10514242)
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| Co-Investigator(Kenkyū-buntansha) |
ITO Kei 北海道大学, 大学院・医学研究科, 非常勤講師 (20421977)
ARITA Ken 北海道大学, 大学院・医学研究科, 非常勤講 師 (50374434)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 皮膚科学 / 表皮水疱症 / 遺伝子治療 / 水疱 / レンチウイルス |
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| Research Abstract |
This study was aimed at developing a new therapy for epidermolysis bullosa, which is one of the severest congenital skin diseases. The study design was utilizing genetic technologies to introduce genes into blisters of disease model animals. Gene introduction was confirmed when the method was applied to cultured cells. However, when it comes to in vivo experiments, introduced genes were not detected in the injected sites. We modified the strategy in which cultured cells were injected into blisters of the disease model animals. We found that the injected cells were functional at the injected sites for at least several days after the treatment.
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[Journal Article] Type VII collagen deficiency causesdefective tooth enamel formation due to poor differentiation of ameloblasts2012
Author(s)
Umemoto H, Akiyama M, Domon T, Nomura T, Shinkuma S, Ito K, Asaka T, Sawamura D, Uitto J, Uo M, Kitagawa Y, Shimizu H
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Journal Title
Am J Pathol
Volume: 181
Pages: 1659-71
DOI
Peer Reviewed
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[Journal Article] Childhood subepidermal blistering disease with autoantibodies against type VII collagen and laminin-3322011
Author(s)
Lin HY, Yanagi T, Akiyama M, Iitani MM, Moriuchi R, Natsuga K, Shinkuma S, Yamane N, Inokuma D, Arita K, Shimizu H
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Journal Title
Br J Dermatol
Volume: 164
Pages: 452-454
DOI
Peer Reviewed
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[Journal Article] A founder effect of c.1938delC in ITGB4 underlies junctional epidermolysis bullosa and its application for prenatal testing2011
Author(s)
Natsuga K, Nishie W, Shinkuma S, Nakamura H, Arita K, Yoneda K, Kusaka T, Yanagihara T, Kosaki R, Sago H, Akiyama M, Shimizu H
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Journal Title
Exp Dermatol
Volume: 20
Pages: 74-76
DOI
Peer Reviewed
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[Journal Article] Prevalent LIPH founder mutations lead to loss of P2Y5 activation ability of PA-PLA1alpha in autosomal recessive hypotrichosis2010
Author(s)
Shinkuma S, Akiyama M, Inoue A, Aoki J, Natsuga K, Nomura T, Arita K, Abe R, Ito K, Nakamura H, Ujiie H, Shibaki A, Suga H, Tsunemi Y, Nishie W, Shimizu H
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Journal Title
Hum Mutat
Volume: 31
Pages: 602-610
DOI
Peer Reviewed
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[Journal Article] Novel IL31RA gene mutation and ancestral OSMR mutant allele in familial primary cutaneous amyloidosis.2010
Author(s)
Lin MW, Lee DD, Liu TT, Lin YF, Chen SY, Huang CC, Weng HY, Liu YF, Tanaka A, Arita K, Lai-Cheong J, Palisson F, Chang YT, Wong CK, Matsuura I, McGrath JA, Tsai SF
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Journal Title
Eur J Hum Genet
Volume: 18
Pages: 26-32
DOI
Peer Reviewed
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[Presentation] インフリキシマブ投与乾癬患者22例および切り替え5症例を含むウステキヌマブ投与患者10例の使用経験2011
Author(s)
伊藤圭,飯谷麻里,渡邊英里香,大宮若菜,豊永愛恋,小玉和郎,小林仁,安田秀美,浜坂幸吉,村本文男,西村真智子
Organizer
第26回日本乾癬学会学術大会大阪
Place of Presentation
大阪国際会議場
Year and Date
20110909-10
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[Presentation] インフリキシマブ投与乾癬患者25例およびバイオロジクススイッチ5症例を含むウステキヌマブ投与患者20例の使用経験2011
Author(s)
伊藤圭,飯谷麻里,渡邊英里香,大宮若菜,豊永愛恋,小玉和郎,小林仁,安田秀美,浜坂幸吉,村本文男,大田光仁,西村真智子
Organizer
第388回日本皮膚科学会北海道地方会
Place of Presentation
札幌、札幌医科大学講堂
Year and Date
2011-12-17
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