Alterations of synaptic transcytosis induced by ethanol exposure: non-vesicular synaptic transport in the rat

2013 Fiscal Year Final Research Report

• Project/Area Number: 22591287
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Psychiatric science
• Research Institution: Kagawa University
• Principal Investigator: TAKEUCHI Yoshiki 香川大学, 医学部, 教授 (20116619)
• Co-Investigator(Kenkyū-buntansha): MIKI Takanori 香川大学, 医学部, 准教授 (30274294) ; IMAGAWA Tomohiro 鳥取大学, 農学部, 教授 (20232605) ; NAKAMURA Kazuhiko 弘前大学, 医学部, 教授 (80263911) ; OHTA Kenichi 香川大学, 医学部, 助教 (50403720) ; SUZUKI Shingo 香川大学, 医学部, 助教 (50451430) ; WARITA Katsuhiko 香川大学, 医学部, 助教 (40452669) ; MATSUMOTO Yoshiki 香川大学, 農学部, 准教授 (90335844)
• Project Period (FY): 2010-04-01 – 2014-03-31
• Keywords: シナプス輸送 / 非小胞性 / アルコール / 離出分泌 / 透出分泌 / タンパク輸送 / 内分泌 / 下垂体後葉

Research Abstract

Neuronal tracer of HRP is considered to be transported independently at synapse. To investigate this transport, the solitary nucleus was observed at the electron microscopic level after WGA-HRP injection into the rat vagus nerve. Animals receiving injection of WGA-HRP with Rab3A-siRNA or alcohol treatment revealed apocrine-like structures of terminals which contained HRP reaction product and indented into secondary neurons. Finally, these structures were observed to be separated completely from axon terminals. Quite similar structures were found in the posterior pituitary in animals receiving same injections into the hypothalamus or alcohol treatment. The apocrine-like structures of hypothalamic terminals showed to contain HRP reaction product and large dense core vesicles, and be frequently separated into the vascular space. The present study apparently confirmed the existence of synaptic transport of proteins which was quite different from previous vesicular synaptic transport.

Research Products

• [Journal Article] Secretion-related structures of hypothalamo-hypophysial terminals in the rat posterior pituitary (2013)
  • Author(s): Takeuchi Y, Liu JQ, Matsumoto Y, Miki T, Ohta K, Warita K, Suzuki S, Tamai M
  • Journal Title: Okajimas Folia Anatomica Japonica Volume: 90(3) Pages: 69-76
  • Peer Reviewed
• [Journal Article] Morphological evidence of an altered process of synaptic transcytosis in adult rats exposed to ethanol (2012)
  • Author(s): Takeuchi Y, Miki T, Liu JQ, Ohta K, Warita K, Matsumoto Y, Suzuki S, Tamai M, Ameno K, Bedi KS, Yakura T
  • Journal Title: Alcohol and Alcoholism Volume: 47(6) Pages: 671-676
  • DOI: 10.1093/alcalc/ags085
  • Peer Reviewed
• [Journal Article] Alteration of the expression levels of Rab3A affects the extent of transcytisis of HRP-Labeled marker proteins in rat CNS neurons (2011)
  • Author(s): Takeuchi Y, Matsumoto Y, Miki T, Warita K, Wang ZY, Bedi KS, Yakura T, Liu JQ
  • Journal Title: Neuroscience and Medicine Volume: 2 Pages: 282-287
  • Peer Reviewed
• [Presentation] Transsynapic transport of WGA conjugated Amyloid-βin the nucleus of solitary tract of the rat (2013)
  • Author(s): Liu JQ
  • Organizer: 第118回日本解剖学会総会・全国学術集会
  • Place of Presentation: サンポートホール高松(高松市)
  • Year and Date: 20130328-30
• [Presentation] Alterations of synaptic transcytosis induced by ethanol exposure : apocrine-likestructure in the rat (2012)
  • Author(s): Yakura T
  • Organizer: 第117回日本解剖学会総会・全国学術集会
  • Place of Presentation: 山梨大学甲府キャンパス(甲府市)
  • Year and Date: 20120326-28
• [Presentation] 下垂体後葉における透出および離出様分泌 (2011)
  • Author(s): 竹内義喜
  • Organizer: Neuroscience 2011/第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 20110914-17