Breakthrough of mechanism in micrometastasis using micrometastatic model of esophageal cancer

2014 Fiscal Year Final Research Report

• Project/Area Number: 22591450
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Gunma University
• Principal Investigator: MIYAZAKI Tatsuya 群馬大学, 医学部附属病院, 助教 (70372349)
• Co-Investigator(Kenkyū-buntansha): YOKOBORI Takehiko 群馬大学, 医学系研究科, 助教 (60420098) ; SAKAI Makoto 群馬大学, 医学部附属病院, 助教 (70420099) ; SOHDA Makoto 群馬大学, 医学部附属病院, 助教 (70507326) ; KUWANO Hiroyuki 群馬大学, 医学系研究科, 教授 (90186560)
• Project Period (FY): 2010-04-01 – 2015-03-31
• Keywords: 微小転移

Outline of Final Research Achievements

We have established mice model to evaluate peritoneal micro metastatic nest, because we could not establish orthotopic transplantation model. We used colon-26-luc cell line and 7-week-old female Balb-c mice. We could visualize micro peritoneal cancer nest as a light of luciferase. We also measured the weight of peritoneal tissue to quantify tumor volume, that related luminescence intensity. We establish the method to evaluate disease progression without sacrifice of mice.

Free Research Field

外科学