2012 Fiscal Year Final Research Report
Human Peritoneal Mesothelial Cell play important roles in the development of peritoneal metastasis by its EMT change
| Project/Area Number |
22591451
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Digestive surgery
|
|---|
| Research Institution | Kanazawa University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HARADA Shinichi 金沢大学, 医学系, 助教 (90272955)
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Keywords | 胃癌 / 腹膜播種 / 腹膜中皮細胞 / EMT |
|---|
| Research Abstract |
We investigated whether TGF-β derived from cancer cells in the peritoneal microenvironment activates human peritoneal mesothelial cells (HPMCs), leading to the progression and fibrosis of gastric cancer. We found that activated HPMCs (a-HPMCs) became more invasive and up-regulated proliferation of human gastric cancer-derived MKN45 cells following direct cell-cell contact. These EMT-like changesof HPMC were suppressed under 5nM of paclitaxel pretreatment by inhibiting Smad2 phosphorylation. To measure the effects of co-culture in vivo, we developed a mouse xenograft model. The largest tumors were observed in mice given MKN45 cells that had been co-cultured with a-HPMCs. Additionaly, these tumors contained HPMC-derived fibrous tissue.
|
