Development of the new therapeutic approach for malignant pleural mesothelioma by the elucidation of aberrant microRNA expression

2012 Fiscal Year Final Research Report

• Project/Area Number: 22591566
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Okayama University
• Principal Investigator: TOYOOKA Shinichi 岡山大学, 岡山大学病院, 講師 (30397880)
• Co-Investigator(Kenkyū-buntansha): MIYOSHI Shinichiro 岡山大学, 大学院・医歯薬学総合研究科, 教授 (00190827) ; TSUKUDA Kazunori 岡山大学, 岡山大学病院, 講師 (20346430) ; ASANO Hiroaki 岡山大学, 岡山大学病院, 助教 (70534775) ; SOH Junichi 岡山大学, 岡山大学病院, 助教 (90559890)
• Project Period (FY): 2010 – 2012
• Keywords: 胸部悪性腫瘍 / 分子腫瘍学 / マイクロRNA

Research Abstract

We detected that miR-34b/c was repressed by methylation in about 85% of malignant mesothelioma. Induction of miR-34b/c into the mesothelioma cell line resulted in the inhibition of the invasiveness and the induction of apoptosis. The in vivo analysis revealed that miR-34b/c had strong antitumor effect. Besides, miR-34b/c enhanced radiosensitivity of the mesothelioma cell line and the repression miR-34b/c caused the increased cell proliferation in human mesotheial cells.

Research Products

• [Journal Article] Downregulation of microRNA-34 induces cell proliferation and invasion of human mesothelial cells (2013)
  • Author(s): Tanaka N, Toyooka S, Soh J, Tsukuda K, Shien K, Furukawa M, Muraoka T, Maki Y, Ueno T, Yamamoto H, Asano H, Otsuki T, Miyoshi S
  • Journal Title: Oncol Rep Volume: 29(6) Pages: 2169-74
  • DOI: doi:10.3892/or.2013.2351
  • Peer Reviewed
• [Journal Article] MicroRNA miR-34b/c enhances cellular radiosensitivity of malignant pleural mesothelioma cells (2012)
  • Author(s): Maki Y, Asano H, Toyooka S, Soh J, Kubo T, Katsui K, Ueno T, Shien K, Muraoka T, Tanaka N, Yamamoto H, Tsukuda K, Kishimoto T, Kanazawa S, Miyoshi S
  • Journal Title: Anticancer Res Volume: 32(11) Pages: 4871-5
  • URL: http://ar.iiarjournals.org/content/32/11/4871.long
  • Peer Reviewed
• [Journal Article] 悪性胸膜中皮腫の新しい分子生物学的異常と治療応用の可能性 (2012)
  • Author(s): 久保孝文、豊岡伸一、三好新一郎
  • Journal Title: 岡山医学会雑誌 Volume: Vol. 124 No. 3 Pages: 203-206
  • URL: https://www.jstage.jst.go.jp/article/joma/124/3/124_203/_article/-char/ja/
  • Peer Reviewed
• [Journal Article] Epigenetic silencing of microRNA-34b/c plays an important role in the pathogenesis of malignant pleural mesothelioma (2011)
  • Author(s): Kubo T, Toyooka S, Tsukuda K, Sakaguchi M, Fukazawa T, Soh J, Asano H, Ueno T, Muraoka T, Yamamoto H, Nasu Y, Kishimoto T, Pass HI, Matsui H, Huh NH, Miyoshi S
  • Journal Title: Clin Cancer Res Volume: 17(15) Pages: 4965-74
  • DOI: doi:10.1158/1078-0432.CCR-10-3040
  • Peer Reviewed
• [Presentation] Down-regulation of microRNA34 induces cell proliferation and invasion of human mesothelial cells (2012)
  • Author(s): Tanaka N, Toyooka S, Soh J, Tsukuda K, Shien K, Furukawa M, Muraoka T, Maki Y, Ueno T, Asano H, Otsuki T, Miyoshi S
  • Organizer: AACR Annual Meeting 2012
  • Place of Presentation: Chicago, USA
  • Year and Date: 20120401-04
• [Presentation] The impact of adenoviral gene transfer of microRNA-34b/c on malignant pleural mesotheliomas. (2011)
  • Author(s): Soh J, Toyooka S, Kubo T, Fukazawa T, Sakaguchi M, Ueno T, Tsukuda K, Asano H, Yamamoto H, Huh, N, Miyoshi S
  • Organizer: AACR Annual Meeting 2011
  • Place of Presentation: Orlando, USA
  • Year and Date: 2011-04-05
• [Presentation] MicroRNA-34b/c is significantly down-regulated by aberrant promoter methylation and forced expression of miR-34b/c enhances the radiosensitivity in malignant pleural mesothelioma cells. (2011)
  • Author(s): Maki Y, Toyooka S, Asano H, Soh J, Tsukuda K, Muraoka T, Aokage K, Tanaka N, Ueno T, Yamane M, Oto T, Miyoshi S
  • Organizer: AACR Annual Meeting 2011
  • Place of Presentation: Orlando, USA
  • Year and Date: 2011-04-05
• [Presentation] 正常中皮細胞におけるmicroRNA34の抑制効果 Tiny RNA drives mesothelial cell mad (2010)
  • Author(s): 田中則光、豊岡伸一、宗 淳一、牧 佑歩、村岡孝幸、上野剛、浅野博昭、佃和憲、三好新一郎
  • Organizer: 日本癌治療学会総会
  • Place of Presentation: 横浜
  • Year and Date: 20101025-27
• [Presentation] Epigenetic silencing of micro-RNA-34b/c plays a pivotal role in pathogenesis of malignant mesothelioma (2010)
  • Author(s): 久保孝文、豊岡伸一、佃 和憲、阪口政清、深澤拓也、宗 淳一、浅野博昭、上野剛、那須康友、松井秀樹、許南浩、三好新一郎
  • Organizer: 第69回日本肺癌学会総会
  • Place of Presentation: 大阪
  • Year and Date: 20100922-24
• [Presentation] Epigenetic silencing of microRNA-34b/c plays a pivotal role in the pathogenesis of malignant pleural mesothelioma (2010)
  • Author(s): Toyooka S, Kubo T, Tsukuda K, Sakaguchi M, Fukazawa T, Soh J, Asano H, Nasu Y, Kishimoto T, Pass H, Matsui H, Huh NH, Miyoshi S
  • Organizer: The 10th International Conference of the International Mesothelioma Interest Group
  • Place of Presentation: 京都
  • Year and Date: 2010-09-01
• [Presentation] Frequent silencing of tumor suppressive miR-34b/c by aberrant methylation in malignant pleural mesothelioma (2010)
  • Author(s): Kubo T, Toyooka S, Tsukuda K, Asano H, Sakaguchi M, Soh J, Ueno T, Yamamoto H, Yamane M, Oto T, Nasu Y, Matsui H, Huh NH, Miyoshi S
  • Organizer: AACR Annual Meeting 2010
  • Place of Presentation: Washington, DC, USA
  • Year and Date: 2010-04-19
• [Remarks]
  • URL: http://www.nigeka-okayama-u.jp