2012 Fiscal Year Final Research Report
Therapeutic effects of transplanted peripheral blood mononuclear cells which mobilized by G-CSF on spinal cord injury in mice
| Project/Area Number |
22591626
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | University of Tsukuba (2012)
Chiba University (2010-2011) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OKAWA Akihiko 千葉大学, 大学院・医学研究院, 講師 (30312945)
MURATA Atsushi 千葉大学, 医学部附属病院, 准教授 (20344997)
FURUYA Takeo 千葉大学, 医学部附属病院, 医員 (00507337)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 脊椎脊髄病学 |
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| Research Abstract |
We analyzed that intraspinal transplantation of CD34+ peripheral blood cells mobilized by granulocyte colony-stimulating factor (G-CSF) facilitates angiogenesis in injured spinal cord and promotes functional recovery after spinal cord injury (SCI). NOD-Scid mice were subjected to contusion-induced SCI. Human CD34+ peripheral blood stem cells (1.0x10^5) mobilized by G-CSF were injected into the injured spinal cords (CD34 group) one week after SCI. Human mononuclear cell fraction (1.0x10^5) mobilized by G-CSF (M group) or saline (Control group) were also administrated. Mice in CD34 and M groups showed significant locomotor recovery of hind limb motor function fromsix to eight weeks after the transplantation (p<0.01). A certain amount of transplanted cells were detected until four weeks, and they were differentiated into vascular endothelial cells. Those results indicate that intraspinal transplantation of human peripheral blood stem cells mobilized by G-CSF facilitates the angiogenesis in injured spinal cord and promotes functional recovery in mice.
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[Journal Article] Granulocyte colony-stimulating factor (G-CSF) protects oligpdendrocyte and promotes hindlimb functional recovery after spinal cord injury in rats2012
Author(s)
Kadota R, Koda M, Kawabe J, HashimotoM, Nishio Y, Mannoji C, Miyashita T, Furuya T, Okawa A, Takahashi K, Yamazaki M
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Journal Title
PLoS One
Volume: 7(11)
Pages: e50391
Peer Reviewed
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[Journal Article] Neuroprotective therapy using granulocyte-colony stimulating factor for patients with worsening symptoms of thoracic myelopathy, a multicenter prospective controlled trial2012
Author(s)
Sakuma T, Yamazaki M, Okawa A, Takahashi H, Kato K, Hashimoto M, Hayashi K, Furuya T, Fujiyoshi T, Kawabe J, Mannoji C, Miyashita T, Kadota R, Someya Y, Ikeda O, Yamauchi T, Hashimoto M, Aizawa T, Ono A, Imagama S, Kanemura T, Hanaoka H, Takahashi K, Koda
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Journal Title
Spine.
Volume: 37(17)
Pages: 1475-1478
Peer Reviewed
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[Journal Article] Neuroprotective therapy using granulocyte colony-stimulating factor for acute spinal cord injury: a phase I/IIa clinical trial2012
Author(s)
Takahashi H, Yamazaki M, Okawa A, Sakuma T, Kato K, Hashimoto M, Hayashi K, Furuya T, Fujiyoshi T, Kawabe J, Yamauchi T, Mannoji C, Miyashita T, Kadota R, Hashimoto M, Ito Y, Takahashi K, Koda M
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Journal Title
Eur Spine J
Volume: 21(12)
Pages: 2580-2587
Peer Reviewed
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[Journal Article] Neuroprotective therapy using granulocyte-colony stimulating factor for patients with worsening symptoms of compression myelopathy, part 1: a phase I and IIa clinical trial2012
Author(s)
Sakuma T, Yamazaki M, Okawa A, Takahashi H, Kato K, Hashimoto M, Hayashi K, Furuya T, Fujiyoshi T, Kawabe J, Mannoji C, Kadota R, Hashimoto M, Takahashi K, Koda M
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Journal Title
Eur Spine J
Volume: 21(3)
Pages: 482-489
Peer Reviewed
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[Journal Article] 急性脊髄損傷および圧迫性脊髄症急性増悪例に対するG-CSF神経保護療法:医師主導型自主臨床試験2012
Author(s)
山崎正志,国府田正雄,佐久間毅,高橋宏,加藤 啓,橋本光宏,林 浩一,川辺純子,藤由崇之,古矢丈雄,山内友規,門田 領,萬納寺誓人,宮下智大,染谷幸男,鎌田尊人,池田 修,橋本将行,須田浩太,楫野知道,上田明希,伊藤康夫,植田尊善,相澤俊峰,小野 睦,今釜史郎,金村徳相,大河昭彦,高橋和久
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Journal Title
日整会誌
Volume: 86
Pages: 891-896
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