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2012 Fiscal Year Final Research Report

Molecular-targeting chemotherapy related to p53-RB for malignant bone and soft tissue tumors

Research Project

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Project/Area Number 22591668
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

TSUJI Yoshiro  京都府立医科大学, 医学研究科, 客員講師 (70453123)

Co-Investigator(Kenkyū-buntansha) MURATA Hiroaki  京都府立医科大学, 医学研究科, 客員講師 (90360031)
Research Collaborator KOTO Kazutaka  明治国際医療大学, 講師 (50649340)
Project Period (FY) 2010 – 2012
Keywords骨肉腫 / 線維肉腫 / スルフォラファン / 化学療法 / 放射線治療 / p53 遺伝子 / アポトーシス
Research Abstract

A MEK inhibitor suppressed cell growth and increased the population of G1/S phase cells in a dose-dependent manner for fibrosarcoma cells. The phosphorylation of ERK was inhibited and p21^WAF1/CIP1 and p27^KIP1 protein expression increased. These results indicate that it might be an attractive compound for molecular-targeting chemotherapy with the activation of the Ras-MEK-ERK pathway.Sulforaphane(SFN) enhanced the radiosensitivity of osteosarcoma cells by inducing apoptosis through G2/M-phase arrest and inhibiting ERK and Akt activation. These findings suggest that SFN can be used as a radiosensitizer for osteosarcomas.

  • Research Products

    (5 results)

All 2013 2012 2010

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (3 results)

  • [Journal Article] Effectiveness of sulforaphane as a radiosensitizer for murine osteosarcoma cells2013

    • Author(s)
      Y. Sawai, H. Murata, M. Horii, K. Koto,T. Matsui, N. Horie, Y. Tsuji, E. Ashihara, T. Maekawa, T. Kubo, S. Fushiki
    • Journal Title

      Oncol Rep

      Volume: 29 Pages: 941-945

    • DOI

      doi:10.3892/or.2012.2195

    • Peer Reviewed
  • [Journal Article] A novel MEK1/2 inhibitor induces G1/S cell cycle arrest in human fibrosarcoma cells2010

    • Author(s)
      T.A. Matsui, H. Murata, Y. Sowa, T. Sakabe, K. Koto, N. Horie, Y. Tsuji, T. Sakai, T. Kubo
    • Journal Title

      Oncol Rep

      Volume: 24 Pages: 329-333

    • DOI

      DOI:10.3892/or_00000863

    • Peer Reviewed
  • [Presentation] Effectiveness of sulforaphane as a radiosensitizer for murine osteosarcoma cells2013

    • Author(s)
      Y. Sawai, H. Murata, M. Horii, K. Koto,T. Matsui, N. Horie, Y. Tsuji, E. Ashihara, T. Maekawa, T. Kubo, S. Fushiki
    • Organizer
      2013 Annual Meeting of the Orthopaedic Research Society
    • Place of Presentation
      San Antonio, USA
    • Year and Date
      20130126-29
  • [Presentation] マウス骨肉腫細胞株に対するsulforaphane の放射線増感作用の検討2012

    • Author(s)
      澤井泰志、村田博昭、小藤和孝、松井隆明、堀江直行、堀井基行、久保俊一
    • Organizer
      第27回日本整形外科学会基礎学術集会
    • Place of Presentation
      名古屋
    • Year and Date
      20121026-27
  • [Presentation] Effect of a specific MEK inhibitor against human fibrosarcoma cells2010

    • Author(s)
      T. Matsui, H. Murata, T. Sakabe, K. Koto, N. Horie, Y. Sowa, T. Sakai, T. Kubo
    • Organizer
      8th Asis Pacific Musculoskeletal Tumor Society Meeting
    • Place of Presentation
      Cebu, Philippines
    • Year and Date
      20100224-27

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Published: 2014-08-29  

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