2012 Fiscal Year Final Research Report
Treatment and measurement of neuropathic pain using acromelic acid analogues
| Project/Area Number |
22591753
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Osaka Medical College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SOEN Masako 大阪医科大学, 医学部, 助教 (90445982)
FUJIWARA Shunsuke 大阪医科大学, 医学部, 助教 (60535813)
MORIMOTO Kenji 大阪医科大学, 医学部, 助教 (20388250)
MIYAZAKI Shinichiro 大阪医科大学, 医学部, 助教 (30411359)
KUSAKA Yusuke 大阪医科大学, 医学部, 助教 (60636075)
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| Co-Investigator(Renkei-kenkyūsha) |
FURUTA Kyoji 岐阜大学, 大学院・医学系研究科再生医科学専攻・医学(系)研究科(研究院), 准教授 (40173538)
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| Project Period (FY) |
2010 – 2012
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| Keywords | アクロメリン酸 / アロディニア / 神経障害性疼痛 |
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| Research Abstract |
We previously showed that intrathecal (i.t.) administration ofacromelic acid A (Acro-A), a major causing substance of the mushroom (Clitocybeacromelalga) poison, provoked allodynia in conscious mice. To clarify a mechanism ofAcro-A, we synthesized [11C]-labeled Acro-A analogue (PSPA-4) that can competitivelyblock the Acro-A-induced allodynia. In vitro autoradiography, [C]PSPA-4 wasspecifically bound to the rat brain and spinal cord, and the binding was significantlydisplaced by kainic acid, but not by antagonists of kainate receptor. PSPA-4 attenuatedthe Acro-A-induced allodynia at low doses and induced allodynia at high doses via a bindingsite different from known kainate antagonists. The development of a radio-labeled Acro-Aanalogue will enable us to promote the understanding of the action mechanism not onlyof Acro-A, but also of pain transmission in the periphery and central nervous system.
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Research Products
(11 results)
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[Journal Article] The action site of the synthenic kainoid(2S, 3R, 4R)-3-carboxymethyl-4(4-methylphenylthio)pyrrolidine-2-carboxylic acid (PSPA-4), an analogue of Japanese mushroom poison acromelic acid, on allodynia (tactile pain).2013
Author(s)
Miyazaki, S., Minami, T., Mizuma, H.,Kanazawa, M., Doi, H., Matsumura, S., Lu,J., Onoe, H., Furuta, K., Suzuki, M., Ito,S.
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Journal Title
Eur. J.Pharmacol.
Volume: 120-127
DOI
Peer Reviewed
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[Journal Article] Proteomic analysis of the cerebrospinalfluid before and after intrathecalinjection of steroid into patients withpostherpetic pain.2013
Author(s)
Lu, J., Katano, T., Nishimura, W.,Fujiwara, S., Miyazaki, S., Okasaki, I.,Aritake K, Urade, Y., Minami, T., ItoS.
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Journal Title
Eur. JPharmacol.
Volume: 120-127
DOI
Peer Reviewed
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[Journal Article] Identification of NIPSNAP1 as anocistatin-interacting proteininvolving pain transmission.2012
Author(s)
Okuda-Ashitaka, E., Minami, T.,Tsubouchi, S., Kiyonari, H., Iwamatsu,A., Noda, T., Handa, H., Ito, S.
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Journal Title
J. Biol. Chem.
Volume: 287
Pages: 10403-10413
DOI
Peer Reviewed
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[Journal Article] Synthesis of an acromelic acid Aanalog-based 11C-labeled PET tracer forexploration of the site of action ofacromelic acid A in allodynia induction.2011
Author(s)
Kanazawa, M., Furuta, K., Doi, H., Mori,T., Minami, T., Ito, S., Suzuki, M.
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Journal Title
Bioorg. Med. Chem. Lett.
Volume: 21
Pages: 2017-2020
DOI
Peer Reviewed
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[Presentation] The action site of the synthenic kainoid(2S, 3R, 4R)-3-carboxymethyl-4-(4-methylphenylthio)pyrrolidine-2-carboxylic acid (PSPA-4), an analogue ofJapanese mushroom poison acromelic acid,for allodynia (tactile pain).2012
Author(s)
Miyazaki, S., Minami, T., Mizuma, H.,Kanazawa, M., Doi, H., Matsumura, S., Lu,J., Onoe, H., Furuta, K., Suzuki, M., Ito,S.
Organizer
The 14th World Congress on Pain. 29
Place of Presentation
Milan
Year and Date
20120800
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[Presentation] Characterization of postherpeticneuralgia in mice with knock-in mutationof NMDA receptor.2012
Author(s)
Ito, S., Sasaki, A., Unezaki, S., Andoh,T., Matsumura, S., Katano, T., Nishio, N.,Nakatsuka, T., Kuraishi, Y., Minami, T.
Organizer
The 14th World Congress on Pain. 31
Place of Presentation
Milan
Year and Date
20120800
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