Invention of prophylactic medicine for premature birth made from soybean.

2012 Fiscal Year Final Research Report

• Project/Area Number: 22591832
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Nara Medical University
• Principal Investigator: SADO Toshiyuki 奈良県立医科大学, 医学部, 講師 (50275335)
• Co-Investigator(Kenkyū-buntansha): KOBAYASHI Hiroshi 奈良県立医科大学, 医学部, 教授 (40178330) ; YOSHIDA Shozo 奈良県立医科大学, 医学部, 助教 (40347555) ; NARUSE Sado 奈良県立医科大学, 医学部, 助教 (70453165)
• Project Period (FY): 2010 – 2012
• Keywords: 産科学

Research Abstract

Premature birth caused poor prognosis in newborn. The aim of this study were to reveal anti-inflammation effect by soybean Kunitz trypsin inhibitor. Soybean Kunitz trypsin inhibitor suppressed the production of inflammatory cytokine. Therefore, soybean Kunitz trypsin inhibitor have anti-inflammation effect.

Research Products

• [Journal Article] The entry of fetal and amniotic fluid components into the uterine vessel circulation leads to sterile inflammatory processes during parturition (2012)
  • Author(s): Kobayashi H
  • Journal Title: Front. Immun. Volume: Vol3
• [Journal Article] Identification of interleukin-6 (IL-6) and squamous cell carcinoma (SCC) as amniotic fluid-specific markers (2012)
  • Author(s): Naruse K, Noguchi T, Yoshida S, Tsunemi T, Shigetomi H, Oi H, Kobayashi H.
  • Journal Title: Open Journal of Obstetrics and Gynecology Volume: 2(2) Pages: 147-150
• [Journal Article] Antiangiogenic-induced hypertension: the molecular basis of signaling network (2012)
  • Author(s): Nagai A, Sado T, Naruse K, Noguchi T, Haruta S, Yoshida S, Tanase Y, Tsunemi T, Kobayashi H
  • Journal Title: Gynecol Obstet Invest Volume: 73(2) Pages: 89-98
• [Journal Article] An Overview of Amniotic Fluid Embolism: Past (2012)
  • Author(s): Tsunemi T, Oi H, Sado T, Naruse K, Noguch T, Kobayashi H
  • Journal Title: Present and Future Directions The Open Women's Health Journal Volume: 6 Pages: 24-29
• [Journal Article] Combination of B-Lynch brace suture and uterine artery embolization for atonic bleeding after cesarean section in a patient with placenta previa accrete (2012)
  • Author(s): Koyama E, Naruse K, Shigetomi H, Sado T, Oi H, Kobayashi H
  • Journal Title: J.Obstet. Gynaecol Volume: Res.38(1) Pages: 345-348
• [Journal Article] Efficacy of inchinkoto for a patient with liver fibrosis complicated with transient abnormal myelopoiesis in Down's syndrome (2011)
  • Author(s): Takeyama M, Uchida Y, Arai I, Kamamoto T, Nishikubo T, Kanehiro H, Sado T, Kunishima S, Takahashi Y
  • Journal Title: Pediatr Int Volume: 53(6) Pages: 1093-6
• [Journal Article] Search for Amniotic Fluid-Specific Markers: Novel Biomarker Candidates for Amniotic Fluid Embolism (2011)
  • Author(s): Kobayashi H, Naruse K, Sado T, Noguchi T, Yoshida S, Shigetomi H, Onogi A, Oi H
  • Journal Title: Open Womens Health J Volume: 5 Pages: 7-15
• [Journal Article] Interaction between uterine natural killer cells and extravillous trophoblast cells: effect on cytokine and angiogenic growth factor production (2011)
  • Author(s): Lash GE, Naruse K, Robson A, Innes BA, Searle RF, Robson SC, Bulmer JN
  • Journal Title: Hum Reprod Volume: 26(9) Pages: 2289-95
• [Journal Article] Increase of high molecular weight adiponectin in hypertensive pregnancy was correlated with brain-type natriuretic peptide stimulation on adipocyte (2011)
  • Author(s): Naruse K, Yamasaki Y, Tsunemi T, Onogi A, Noguchi T, Sado T, Oi H, Kobayashi H
  • Journal Title: Pregnancy Hypertension Volume: 1 Pages: 200-205
• [Journal Article] Inflammatory pattern recognition receptors and thieir ligands:factors contributing to the pathogenesis of preeclampsia Inflamm (2011)
  • Author(s): Sado T, Naruse K, Noguchi T, Haruta S, Yoshida S, Tanase Y, Kitanaka T, Oi H, Kobayashi H
  • Journal Title: Res Volume: 60 Pages: 509-520
• [Journal Article] Hypoxia inhibits invasion of extravillous trophoblast cells through reduction of matrix metalloproteinase (MMP)-2 activation in the early first trimester of human pregnancy (2011)
  • Author(s): Onogi A, Naruse K, Sado T, Tsunemi T, Shigetomi H, Noguchi T, YamadaY, Akasaki M, Oi H, Kobayashi H
  • Journal Title: Placenta Volume: 32 Pages: 665-670
• [Journal Article] Anticytokine Therapy in Preterm Labor: Current Knowledge and Future Perspectives (2011)
  • Author(s): Sado T, Kitanaka T, Naruse K, Oi H, Noguchi T, Yoshida S, Kajihara H, Shigetomi H, Oonogi A, Kobayashi H
  • Journal Title: Gynecol Obstet Invest Volume: 71(1) Pages: 1-10
• [Journal Article] A new approach regarding the treatment of preeclampsia and preterm labor (2011)
  • Author(s): Mizutani S, Wright J, Kobayashi H
  • Journal Title: Life Sci Volume: 88(1-2) Pages: 17-23
• [Journal Article] Effect of tumour necrosis factor-α in combination with interferon-γ on first trimester extravillous trophoblast invasion (2011)
  • Author(s): Otun HA, Lash GE, Innes BA, Bulmer JN, Naruse K, Hannon T, Searle RF, Robson SC
  • Journal Title: J Reprod Immunol Volume: 88(1) Pages: 1-11
• [Journal Article] Evidence for activation of toll-like receptor and receptor for advanced glycation end products in preterm birth (2010)
  • Author(s): Noguchi T, Sado T, Naruse K, Shigetomi H, Onogi A, Haruta S, Kawaguchi R, Nagai A, Tanase Y, Yoshida S, Kitanaka T, Oi H, Kobayashi H
  • Journal Title: Mediators Inflamm Volume: 2010 Pages: 1-10
• [Journal Article] Anti-inflammatory actions of serine protease inhibitors containing the Kunitz domain (2010)
  • Author(s): Shigetomi H, Oonogi A, Kajiwara H, Yoshida S, Furukawa N, Haruta S, Tanase Y, Kanayama S, Noguchi T, Yamada Y. Oi H. Kobayashi H
  • Journal Title: Inflamm Res Volume: 59(9) Pages: 679-687
• [Journal Article] Fatal Factors of Clinical Manifestations and Laboratory Testing in Patients with Amniotic Fluid Embolism Gynecol Obstet Invest (2010)
  • Author(s): Oi H. Naruse K, Noguchi T, Sado T,Kimura S, Kanayama S,Terao T, Kobayashi H
  • Volume: 70(2)
  • Pages: 138-144
• [Journal Article] Secretion of cytokines by villous cytotrophoblast and extravillous trophoblast in the first trimester of human pregnancy (2010)
  • Author(s): Naruse K, Innes BA, Bulmer JN, Robson SC, Searle RF, Lash GE
  • Journal Title: J Reprod Immunol Volume: 86(2) Pages: 148-150
• [Journal Article] Secretion of angiogenic growth factors by villous cytotrophoblast and extravillous trophoblast in early human pregnancy (2010)
  • Author(s): Lash GE, Naruse K, Innes BA, Robson SC, Searle RF, Bulmer JN
  • Journal Title: Placenta Volume: 31(6) Pages: 545-548
• [Presentation] Apoptotic and Necrotic Cell Death of Trophoblast and Adipose Tissue on Hypoxia and Preeclampsia-A Preliminary (2012)
  • Author(s): Naruse K, Tsunemi T, Sado T, Akasaka J, Koike N, Ito F, Noguchi T, Oi H, Kobayashi H
  • Organizer: Observation.International Federation of Placenta Associations Meeting 2012
  • Place of Presentation: Hiroshima
  • Year and Date: 20120918-21
• [Presentation] Hypoxia Increases Urokinase-Type Plasminogen Activator Receptor (uPAR) and Hypoxia Induced Factor (HIF)-1 Alpha in Early Human Extravillous Trophoblast (2012)
  • Author(s): Tsunemi T, Naruse K, Akasaki M, Sado T, Noguchi T, Oi H, Kobayashi H
  • Organizer: International Federation of Placenta Associations Meeting 2012
  • Place of Presentation: Hiroshima
  • Year and Date: 20120918-21
• [Presentation] 新たな脂肪組織培養系を用いた正常妊娠・妊娠高血圧における細胞傷害機序の推測 (2012)
  • Author(s): 成瀬勝彦, 常見泰平, 赤坂珠理晃, 小池奈月, 野口武俊, 佐道俊幸, 大井豪一,小林 浩
  • Organizer: 第36回日本産科婦人科栄養・代謝研究会
  • Place of Presentation: 鹿児島
  • Year and Date: 20120823-24
• [Presentation] ヒト妊娠初期絨毛細胞における低酸素環境によるuPA系制御に関する検討 (2012)
  • Author(s): 常見泰平, 成瀬勝彦, 大野木 輝, 赤坂珠理晃, 佐道俊幸, 大井豪一,赤崎正佳,小林 浩
  • Organizer: 第17回日本病態プロテアーゼ学会
  • Place of Presentation: 浜松
  • Year and Date: 20120810-11
• [Presentation] Necrotic and apoptotic death of adipocyte induced in normal pregnancy and preeclampsia: Findings under novel culture method of adipose tissue (2012)
  • Author(s): Naruse K, Tsunemi T, Onogi A, Koike N, Noguchi T, Sado T, Oi H, Kobayashi H
  • Organizer: 18th World congress of the International Society for the Study of Hypertension in Pregnancy (ISSHP)
  • Place of Presentation: Geneva, Switzerland
  • Year and Date: 20120709-12
• [Presentation] 前回妊娠時に深部静脈血栓症の既往がある先天性アンチトロンビンIII欠乏症合併妊娠の 1 例 (2012)
  • Author(s): 佐道俊幸, 小池奈月, 植栗千陽,金山清二, 成瀬勝彦, 野口武俊, 高橋幸博,小林 浩
  • Organizer: 第22回日本産婦人科・新生児血液学会
  • Year and Date: 20120629-30
• [Presentation] 登録された羊水塞栓症例における症状別解析より本症の発症原因を探る (2012)
  • Author(s): 大井豪一,佐道俊幸, 野口武俊, 金山清二, 成瀬勝彦, 常見泰平, 小山恵美, 小池奈月, 小川憲二,河原直紀,小林 浩,金山尚裕
  • Organizer: 第64回日本産科婦人科学会学術講演会
  • Year and Date: 20120413-15
• [Presentation] 正常妊娠および妊娠高血圧腎症における血清中 RAGE 作用物質の動態と炎症への作用 (2012)
  • Author(s): 成瀬勝彦, 野口武俊, 佐道俊幸, 常見泰平, 赤坂珠理晃, 小池奈月, 大井豪一,小林 浩
  • Organizer: 第64回日本産科婦人科学会学術講演会
  • Year and Date: 20120413-15
• [Presentation] 低酸素環境における妊娠初期絨毛細胞の線溶系因子に及ぼす影響 (2012)
  • Author(s): 常見泰平, 成瀬勝彦, 大野木 輝, 金山清二, 野口武俊, 大井豪一,小林 浩
  • Organizer: 第64回日本産科婦人科学会学術講演会
  • Year and Date: 20120413-15
• [Presentation] 正常妊娠および妊娠高血圧症候群における RAGE 作用物質および炎症関連物質の動態 (2011)
  • Author(s): 成瀬勝彦, 野口武俊, 佐道俊幸, 常見泰平, 小山恵美, 小池奈月, 大井豪一, 小林 浩
  • Organizer: 第26回日本生殖免疫学会
  • Place of Presentation: 名古屋
  • Year and Date: 20111202-03
• [Presentation] 正常妊娠経過中のAGEs/RAGE 系変動と妊娠糖尿病ならびに高血圧への関与 (2011)
  • Author(s): 成瀬勝彦、常見泰平、小山恵美、小池奈月、金山清二、野口武俊、佐道俊幸、大井豪一、小林 浩
  • Organizer: 第27回日本糖尿病・妊娠学会
  • Place of Presentation: 神戸
  • Year and Date: 20111111-12
• [Presentation] 妊娠初期絨毛細胞の低酸素刺激によるプロテアーゼ系調節機構:MMP-TIMP 複合体の調節 (2011)
  • Author(s): 成瀬勝彦, 大野木輝, 常見泰平, 重富洋志, 野口武俊, 金山清二, 佐道俊幸, 大井豪一, 小林 浩
  • Organizer: 第19回日本胎盤学会
  • Place of Presentation: 東京
  • Year and Date: 20110930-1001
• [Presentation] REGURATION OF INVASION BY OXYGEN IN PRIMARY EXTRAVILLOUS TROPHOBLAST AND CHORIOCARCINOMA CELL LINE (2011)
  • Author(s): Naruse K, Onogi A, Tsunemi T, Akasaki M, Sado T, Noguchi T, Yamada Y, Oi H, Kobayashi H
  • Organizer: International Federation of Placenta Associations Meeting 2011(IFPA)
  • Place of Presentation: Geilo, Norway
  • Year and Date: 20110914-17
• [Presentation] HYPOXIA DECREASES MATRIX MEETALLOPROTEINASE(MMP)-2 ACTIVATION WITHOUT INCREASING HYPOXIA INDUCED FACTOR(HIF)-1ALPHA IN HUMAN TROPHOBLAST (2011)
  • Author(s): Tsunemi T, Onogi A, Naruse K, Akasaki M, Sado T, Noguchi T, Yamada Y, Oi H, Kobayashi H
  • Organizer: nternational Federation of Placenta Associations Meeting 2011(IFPA)
  • Place of Presentation: Geilo, Norway
  • Year and Date: 20110914-17
• [Presentation] 致死的因子を含む割合が関連する羊水塞栓症における死亡率 (2011)
  • Author(s): 大井豪一, 佐道俊幸, 野口武俊, 成瀬勝彦, 大野木 輝, 常見泰平, 西岡和弘、岩井加奈, 山田有紀, 小林 浩, 木村 聡, 金山尚裕
  • Organizer: 第63回日本産科婦人科学会総会学術講演会
  • Place of Presentation: 大阪
  • Year and Date: 20110829-31
• [Presentation] 低酸素環境が妊娠初期絨毛細胞のmatrix metalloproteinase2(MMP2)活性化と浸潤能に及ぼす影響とその調節機構の解明 (2011)
  • Author(s): 大野木 輝, 成瀬勝彦, 重富洋志,常見泰平, 野口武俊, 佐道俊幸, 山田嘉彦, 赤崎正佳, 大井豪一, 小林 浩
  • Organizer: 第63回日本産科婦人科学会総会学術講演会
  • Place of Presentation: 大阪
  • Year and Date: 20110829-31
• [Presentation] 生体由来妊娠初期絨毛細胞の低酸素環境下におけるプロテアーゼ活性の検討 (2011)
  • Author(s): 大野木輝、成瀬勝彦、常見泰平、佐道俊幸、重富洋志、赤崎正佳、大井豪一、小林浩
  • Organizer: 第16回日本病態プロテアーゼ学会
  • Place of Presentation: 大阪
  • Year and Date: 20110826-27
• [Presentation] 低酸素環境における妊娠初期絨毛細胞のプロテアーゼ産出と浸潤能の変化に関する検討 (2010)
  • Author(s): 大野木 輝、成瀬勝彦、野口武俊, 佐道俊幸, 山田嘉彦, 北中孝司, 大井豪一,小林 浩
  • Organizer: 第31回日本妊娠高血圧学会
  • Place of Presentation: 東京
  • Year and Date: 20101015-16
• [Presentation] Correlation of free fatty acid and adiponectin in hypertensive pregnancy:key molecules for homeostatic inflammatory linkage (2010)
  • Author(s): Naruse K, Onogi A, Sado T, Noguchi T, Komeda S, Koyama E, Nishioka K, Kitanaka T, Oi H, Kobayashi H
  • Organizer: 17th World congress of the International Society for the Study of Hypertension in Pregnancy (ISSHP)
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 20101004-06
• [Presentation] 低酸素環境での妊娠初期絨毛細胞のプロテアーゼ産出と浸潤能に関する検討 (2010)
  • Author(s): 大野木輝、成瀬勝彦、野口武俊, 佐道俊幸, 北中孝司, 大井豪一, 小林 浩
  • Organizer: 第18回日本胎盤学会学術集会
  • Place of Presentation: 熊本
  • Year and Date: 20100930-1001
• [Presentation] 正常妊娠と妊娠高血圧症における遊離脂肪酸(FFA)とアディポネクチンの連関 (2010)
  • Author(s): 成瀬勝彦、大野木輝、小山恵美、米田聡美、西岡和弘、野口武俊、佐道俊幸、北中孝司、小林 浩
  • Organizer: 第34回日本産科婦人科栄養・代謝研究会
  • Place of Presentation: 三重
  • Year and Date: 20100903-04
• [Presentation] The role of a low oxygen environment on the invasion of extravillous trophoblast from first trimester of pregnancy (2010)
  • Author(s): 大野木 輝, 成瀬勝彦, 佐道俊幸, 重富洋志, 山田嘉彦,赤崎正佳, 大井豪一, 小林 浩
  • Organizer: 第25回日本生殖免疫学会
  • Place of Presentation: 大阪
  • Year and Date: 20100828-29
• [Presentation] Invasive activities of primary extravillous trophoblast via altered proteinases activity in low oxygen condition (2010)
  • Author(s): Naruse K, Onogi A, Noguchi T, Sado T, Kitanaka T, Oi H, Kobayashi H
  • Organizer: XIth International Congress of Reproductive Immunology (ICRI)
  • Place of Presentation: Cairns, Australia
  • Year and Date: 20100815-19
• [Presentation] 妊娠中の遊離脂肪酸(FFA)変動ならびにアディポサイトカインとの連関 (2010)
  • Author(s): 成瀬勝彦、大野木 輝、常見泰平、野口武俊、佐道俊幸、北中孝司、大井豪一、小林 浩
  • Organizer: 第26回日本糖尿病・妊娠学会学術集会
  • Place of Presentation: 埼玉
  • Year and Date: 2010-11-27
• [Presentation] 切迫早産妊婦の居住環境とストレスに関する研究 (2010)
  • Author(s): 佐道俊幸, 小林 浩, 吉川紀子, 松村広恵, 堂本万起, 坂本信子, 町井淳子, 西浦真千代
  • Organizer: 2009年度「病室環境研究」成果報告会
  • Place of Presentation: 橿原
  • Year and Date: 2010-08-20