2012 Fiscal Year Final Research Report
The effect of estrogen for carbohydrate metabolism in pancreatic beta cells
| Project/Area Number |
22591853
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KOMATSU Mitsuhisa 信州大学, 医学部, 教授 (90221978)
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| Project Period (FY) |
2010 – 2012
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| Keywords | エストロゲン / 膵β細胞 / 転写調節因子 / インスリンプロモーター |
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| Research Abstract |
We have demonstrated the treatment of HIT-T15 insulinoma cellswith E2 led to the decreased insulin mRNA levels. Transient transfection assays of HIT-T15 cells revealed that ERα repressed transcriptional activity of rat insulin II promoter bothin a ligand-dependent and -independent manner. The repression was not mediated by an ERE-like sequence or AP-1 site in the promoter. Instead, transcriptional factor PDX-1 and BETA2 appear to be involved.
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