2012 Fiscal Year Final Research Report
RNA expression in facial muscle following damage to the facial nerve -As a tool of early prognostic diagnosis-
| Project/Area Number |
22591884
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Ehime University |
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Principal Investigator |
HATO Naohito 愛媛大学, 大学院・医学系研究科, 准教授 (60284410)
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| Co-Investigator(Kenkyū-buntansha) |
WAKISAKA Hiroyuki 愛媛県立医療技術大学, 保健科学部, 教授 (30304611)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 顔面神経 / 神経挫滅 / マイクロアレイ法 / RNA解析 |
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| Research Abstract |
This study investigated and identified differentially expressed facial muscle genes in rats with facial nerve damage. Two types of adult Wistar rats: 1) the main trunk of the facial nerve was transected(Group A) and 2) the main trunk was compressed for ten minutes (Group B) wereused for the analysis. Global gene expressions of facial muscles were analyzed using a micro-array system. A total of 26,835 RNA molecules were examined. Myogenin mRNA expression increased to the highest level at 7 days after denervation, thereafter it gradually decreased in Group A. Vesicle-associated membrane protein 2 and insulin-like growth factor binding protein 6 expression increased at 7 and 14 days in Group A. The gene expression was different depending on the degree of the facial nerve damage. Therefore, we concluded that analysis of the facial muscle profile gene expression could be a possible diagnostic tool for use in asthe prognosis after facial nerve injury.
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