2012 Fiscal Year Final Research Report
The research of pathogenesis, development's mechanism and treatment of Pars Tensa Cholesteatoma.
| Project/Area Number |
22591891
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
UCHIMIZU Hirotaka 東京慈恵会医科大学, 医学部, 講師 (00307414)
SHIWA Masanori 東京慈恵会医科大学, 医学部, 准教授 (20235766)
YOSHIKAWA Mamoru 東京慈恵会医科大学, 医学部, 講師 (50277092)
YAMAMOTO Kazuhisa 東京慈恵会医科大学, 医学部, 助教 (50408449)
TANAKA Yasuhiro 東京慈恵会医科大学, 医学部, 講師 (40266648)
KOJIMA Hiromi 東京慈恵会医科大学, 医学部, 准教授 (60234762)
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| Project Period (FY) |
2000 – 2012
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| Keywords | 緊張部型真珠腫 / 中耳粘膜再生 / 遺伝子解析 / 中耳腔ガス換気能 / 術後成績 表皮増殖機序 / 線維芽細胞 / キャスペース 14 |
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| Research Abstract |
Cartilage tympanoplasty for Pars tensa(PT) cholesteatoma prevent postoperative retraction ofn tympanic membrane in the long term, and it was found that the hearing improvement was good. There are some relations between Otisis media with effusion and PT-cholesteatoma. It was thought that fibroblasts in subepithelial layer were involved in activity of cholesteatoma epithelium and cholesteatoma extension. The interaction of epithelium and the subepithelial tissue play ley role for development of cholesteatoma. We cultured a rabbit nasal mucosa epithelial cells sheet in temperature-responsive culture plate by animal experiment, and we transplanted it to the middle ear, and good mucosa regenaration was confirmed. Regenerated mucosa shows almost normal conditions with a histologically and functionally(pressure measurement of the middle ear cavity). Weconfirm that Caspase-14 where 1.170 is associated with for the differentiation of epithelial cells specifically develops in cholesteatoma epithelial cells. It is suggested that Caspase-14 promoter helped treatment (disappearance of the persistentcholesteatoma epithelium) of the middle ear cholesteatoma.
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