Molecular mechanisms of ghost cell formation and calcification in calcifying cystic odontogenic tumor (CCOT) cell lines

2012 Fiscal Year Final Research Report

• Project/Area Number: 22592033
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Niigata University
• Principal Investigator: CHENG Jun 新潟大学, 医歯学系, 准教授 (40207460)
• Co-Investigator(Kenkyū-buntansha): MARUYAMA Satoshi 新潟大学, 医歯学総合病院, 講 師 (30397161) ; YAMAZAKI Manabu 新潟大学, 医歯学系, 助 教 (10547516) ; SAKU Takashi 新潟大学, 医歯学系, 教 授 (40145264) ; ABE Tatsuya 新潟大学, 医歯学総合病院, レジデント (70634856)
• Project Period (FY): 2010 – 2012
• Keywords: 歯原性腫瘍 / 石灰化歯原性嚢胞 / 幻影細胞 / 石灰化 / 細胞外基質 / 細胞培養 / 脱核 / 免疫組織化学 / RT-PCR

Research Abstract

Calcifying cystic odontogenic tumor (CCOT) is histopathologically characterized by the emergence of ghost cells. However, their histopathogenesis has been poorly understood. To understand the cellular mechanism towards ghost cell formation, we have successfully established of cell lines from a CCOT surgical specimen. Using these CCOT cells, we successfully demonstrated that ghost cells were generated due to intracellular deposits of extracellular matrix molecules including perlecan, and that their calcification was started in those matrices and completed by proteolysis of deposited matrices towards their denucleated forms.

Research Products

• [Journal Article] Intramuscular keratocyst as a soft tissue counterpart of keratocystic odontogenic tumor : differential diagnosis by immunohistochemistry (2014)
  • Author(s): Abe T, Maruyama S, Yamazaki M, Essa A, Babkair H, Mikami T, Shingaki S, Kobayashi T, Hayashi T, Cheng J, Saku T
  • Journal Title: Human Pathology Volume: 45(1) Pages: 110-118
  • DOI: DOI:j.humanpath.2013.08.011
  • Peer Reviewed
• [Journal Article] Ghost cell odontogenic carcinoma arising in the background of a benign calcifying cystic odontogenic tumor of the mandible (2012)
  • Author(s): Arashiyama T, Kodama Y, Kobayashi T, Hoshina H, Takagi R, Hayashi T, Cheng J, Saku T
  • Journal Title: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology Volume: 114(3) Pages: e35-40
  • DOI: DOI:10.1016/j.oooo.2012.01.018
  • Peer Reviewed
• [Journal Article] Parenchymal-stromal switching for extracellular matrix production on invasion of oral squamous cell carcinoma (2012)
  • Author(s): Metwaly H, Maruyama S, Yamazaki M, Tsuneki M, Abe T, Jen KY, Cheng J, Saku T
  • Journal Title: Human Pathology Volume: 43 (11) Pages: 1973-1981
  • DOI: DOI:10.1016/j.humanpath.2012.02.006
  • Peer Reviewed
• [Journal Article] Podoplanin expression profiles characteristic of odontogenic tumor-specific tissue architectures (2012)
  • Author(s): Tsuneki M, Maruyama S, Yamazaki M, Cheng J, Saku T
  • Journal Title: Pathology - Research and Practice Volume: 208 (3) Pages: 140-146
  • DOI: DOI: org/10.1016/j.prp.2011.12.016
  • Peer Reviewed
• [Journal Article] Combined immunohistochemistry for the differential diagnosis of cystic jaw lesions : its practical in surgical pathology (2010)
  • Author(s): Tsuneki M, Yamazaki M, Cheng J, Maruyama S, Kobayashi T, Saku T
  • Journal Title: Histopathology Volume: 57(6) Pages: 806-813
  • DOI: DOI:10.1111/j.1365-2559. 2010.03721.x
  • Peer Reviewed
• [Presentation] 側頭筋-咬筋内嚢胞性病変 (2012)
  • Author(s): 阿部達也,丸山 智,山崎 学,アーメッド エッサ,新垣 晋,齊藤 力,林 孝文,程 〓,朔 敬
  • Organizer: 第23回日本臨床口腔病理学会総会・学術大会
  • Place of Presentation: 東京医科歯科大学M&D タワー鈴木章夫記念講堂 (東京都文京区)
  • Year and Date: 20120829-31
• [Presentation] A clinicopathological analysis of intraductal papilloma of minor salivary gland origin (2012)
  • Author(s): Cheng J, Wu L, Maruyama S, Yamazaki M, Saku T
  • Organizer: Japan-China Dental Conference 2012
  • Place of Presentation: West China College of Stomatology, Sichuan University (Chengdu, China)
  • Year and Date: 20120426-28
• [Presentation] Intraductal papilloma with peri-luminal extension of the minor salivary gland (2011)
  • Author(s): Cheng J, Wu L, Maruyama S, Yamazaki M, Saku T
  • Organizer: 第22回日本臨床口腔病理学会総会・学術大会・第5回アジア口腔病理学会
  • Place of Presentation: 九州大学医学部百年講堂 (福岡市東区)
  • Year and Date: 20110823-25
• [Presentation] Combined immunohistochemistry for the differential diagnosis of cystic jaw lesions: its practical in surgical pathology (2010)
  • Author(s): Tsuneki M, Yamazaki M, Cheng J, Maruyama S, Saku T
  • Organizer: 15^<th> International Congress on Oral Pathology and Medicine. International Association of Oral Pathologists 2010 in Seoul
  • Place of Presentation: Seoul COEX (Seoul, Korea)
  • Year and Date: 20100816-20
• [Presentation] 顎骨腫瘍 (2010)
  • Author(s): 程 〓,常木雅之,丸山 智,山崎 学,金丸祥平,新垣 晋,齊藤 力,西山秀昌,林 孝文,朔 敬
  • Organizer: 第20回日本口腔粘膜病学会・第21回日本臨床口腔病理学会総会・学術大会
  • Place of Presentation: 大阪歯科大学楠葉学舎 (大阪府枚方市)
  • Year and Date: 20100730-0801
• [Presentation] 角化嚢胞性歯原性腫瘍におけるパールカン結合分子 (2010)
  • Author(s): 長谷川真弓,程 〓,丸山 智,山崎学,齊藤 力,朔
  • Organizer: 第20回日本口腔粘膜病学会・第21回日本臨床口腔病理学会総会・学術大会
  • Place of Presentation: 大阪歯科大学楠葉学舎(大阪府枚方市)
  • Year and Date: 20100730-0801
• [Remarks]
  • URL: http://www.dent.niigata-u.ac.jp/patho/publications/index.html