Paradigm‐shift of the stem cell niche of taste buds cells

2012 Fiscal Year Final Research Report

• Project/Area Number: 22592061
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: OTA Masato 東京医科歯科大学, 大学院・医歯学総合研究科, 講師 (70313228)
• Co-Investigator(Kenkyū-buntansha): KONDOH Takashi 独立行政法人理化学研究所, 免疫アレルキ゛ーセンター, 研究員 (40333299)
• Project Period (FY): 2010 – 2012
• Keywords: 味蕾幹細胞 / BrdU標識長期維持細胞 / 遺伝子群の検索 / Hes1 / Meis2

Research Abstract

The epithelial stem cells that originally have been thought to make glands that secrete saliva and mucous membranes of the mouth. Here, we suggest that these stem cells also make cellsfeeling the taste in the mouth that locate around the tongue, back part of the upper jaw and throat. We observed that these cells have the very slow rate of cell growth and the expression ofseveral genes that is known ascharacteristic feature of the stem cells. Further, the cells of themucosa of the mouth with such genes was also suggested to play an important role as a stem cell to make the tooth roots.

Research Products

• [Journal Article] Promotional effects of vasoactive intestinal peptide on the development of rodent Hertwig's epithelial root sheath (2012)
  • Author(s): Xu J, Kawashima N, Fujiwara N, Harada H, Ota MS, Suda H
  • Journal Title: Congenit Anom (Kyoto) Volume: Vol.52、No.3 Pages: 162-7
  • Peer Reviewed
• [Journal Article] Cholesteryl group- and acryloylgroup-bearing pullulan nanogel to deliver BMP2 and FGF18 for bone tissue engineering (2012)
  • Author(s): Fujioka-Kobayashi M, Ota MS, Shimoda A, Nakahama K, Akiyoshi K, Miyamoto Y, Iseki S
  • Journal Title: Biomaterials Volume: Vol.3、No.30 Pages: 7613-20
  • DOI: doi:10.1111/j.1600-0765.2011.01419.x
  • Peer Reviewed
• [Journal Article] Restricted expression of chromatin remodeling associated factor Chd3 during tooth root development. J (2012)
  • Author(s): Date Y, Yokoyama Y, Kondo H, Kuroda S, Ohya K, Ota MS, Iseki S, Kasugai S
  • Journal Title: Periodontal Res Volume: Vol.47、No.2 Pages: 180-7
  • DOI: doi:10.1111/j.1600-0765.2011.01419.x
  • Peer Reviewed
• [Journal Article] Ectoderm, endoderm, and the evolution of heterodont dentitions (2010)
  • Author(s): Ohazama A, Haworth KE, Ota MS, Khonsari RH, Sharpe PT
  • Journal Title: Genesis Volume: Vol.48、No.6 Pages: 382-9
  • DOI: doi:10.1002/dvg.20634
  • Peer Reviewed
• [Journal Article] Lrp4: A novel modulator of extracellular signaling in craniofacial organogenesis (2010)
  • Author(s): Ohazama A, Porntaveetus T, Ota MS, Herz J, Sharpe PT
  • Journal Title: Am J Med Genet A Volume: Vol.152A、No.12 Pages: 2974-83
  • DOI: doi:10.1002/ajmg.a.33372. Review
  • Peer Reviewed
• [Journal Article] A role for suppressed incisor cuspalmorphogenesis in the evolution of mammalian heterodont dentition (2010)
  • Author(s): Ohazama A, Blackburn J, Porntaveetus T, Ota MS, Choi HY, Johnson EB, Myers P, Oommen S, Eto K, Kessler JA, Kondo T, Fraser GJ, Streelman JT, Pardinas UF, Tucker AS, Ortiz PE, Charles C, Viriot L, Herz J, Sharpe PT
  • Journal Title: Proc Natl Acad Sci U S A Volume: Vol.107、No.1 Pages: 92-7
  • Peer Reviewed
• [Presentation] Combined in silico and in vivo analyses reveal role of Hes1 in taste cell differentiation (2012)
  • Author(s): Ota MS, Kaneko Y, Kondo K, Ogishima S, Tanaka H, Kondo T
  • Organizer: HSDB symposium
  • Year and Date: 20121126-27
• [Presentation] 新規徐放性ナノゲルを用いたFGF18によるBMP2依存的な骨修復能の改良 (2011)
  • Author(s): 小林真左子、太田正人、下田麻子、秋吉一成、宮本洋二、井関祥子
  • Organizer: 第31回日本炎症・再生医学会
  • Place of Presentation: 京都
  • Year and Date: 20110602-03
• [Presentation] Biological effects of natural products on tooth root formation (2011)
  • Author(s): 太田正人
  • Organizer: 平成22年度戦略的研究基盤形成支援事業研究集会
  • Place of Presentation: 東京
  • Year and Date: 20110220-21
• [Presentation] FGFRの象牙質形成における発現 (2011)
  • Author(s): 馬場麻人、寺島達夫、太田正人、田畑純、高野吉郎
  • Organizer: 第58回国際歯科研究学会日本部会(JADR)総会・学術大会
  • Place of Presentation: 小倉
  • Year and Date: 2011-11-20
• [Presentation] 神経堤細胞特異的Meis2ノックアウトマウスにおける表現型解析 (2011)
  • Author(s): 太田正人、井関祥子
  • Organizer: 第53回歯科基礎医学会学術大会・総会
  • Place of Presentation: 岐阜
  • Year and Date: 2011-10-02
• [Presentation] 長寿マウスの上顎切歯に特有の加齢現象についてサテライトシンポジウム「エナメル質研究の温故知新-新たな展開を期して」 (2011)
  • Author(s): 太田正人
  • Organizer: 第53回歯科基礎医学会学術大会・総会
  • Place of Presentation: 岐阜
  • Year and Date: 2011-09-30
• [Presentation] マウス上顎切歯をモデルとした加齢にともなう遺伝子. 発現調節機構の改変の解析 (2010)
  • Author(s): 太田正人、近藤香、井関祥子、近藤隆
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 20101207-10
• [Presentation] 歯の比較解剖学と再生医学との接点. 歯の比較解剖学と再生医学との接点 (2010)
  • Author(s): 小澤幸重、馬場麻人、太田正人
  • Organizer: 第52回歯科基礎医学会学術総会
  • Place of Presentation: 東京
  • Year and Date: 20100920-22
• [Presentation] 象牙芽細胞におけるFGFRの発現 (2010)
  • Author(s): 馬場麻人、寺島達夫、太田正人、田畑純、 高野吉郎
  • Organizer: 第52回歯科基礎医学会学術総会
  • Place of Presentation: 東京
  • Year and Date: 20100920-22
• [Presentation] 1Biological effects of cyclic diarylheptanoids on tooth root formation (2010)
  • Author(s): Lee JW., Ota MS., Fujiwara N., Yonezawa T., Date Y., Harada H., Iseki S., Seo HJ., Jeon WB., Cha BY.1, Nagai K., Woo JT
  • Organizer: The 10th Tooth Morphogenesis and Differentiation
  • Place of Presentation: Berlin
  • Year and Date: 20100901-04
• [Presentation] The role of Wnt signaling for patterning of molar tooth roots in mammals (2010)
  • Author(s): Ota MS., Nakahara T., Ohazama A., Schmidt-Ullrich R., Kanri Y., Kozawa Y., Aoba T., Sharpe P.T., Kondo T., Iseki S
  • Organizer: The 10th Tooth Morphogenesis and Differentiation
  • Place of Presentation: Berlin
  • Year and Date: 20100901-04
• [Presentation] Genome-wide screening of key molecules for tooth root development (2010)
  • Author(s): Date Y., Ota MS., Yokoyama Y., Iseki S., Kasugai S
  • Organizer: The 10th Tooth Morphogenesis and Differentiation
  • Place of Presentation: Berlin
  • Year and Date: 20100901-04
• [Presentation] FGFシグナルの胎児マウス頭蓋冠骨芽細胞分化に与える影響 (2010)
  • Author(s): 永山友子、中原貴、太田正人、春日井昇平、井関祥子
  • Organizer: 第30回日本炎症・再生医学会
  • Place of Presentation: 東京
  • Year and Date: 20100805-06
• [Presentation] Wnt-signaling pathway is important for patterning of mammalian tooth roots in evolution and development (2010)
  • Author(s): Ota MS, Itoh N., Hiroki Kokubo H , Ohazama A, Kozawa Y,Saga Y, Sharpe PT, KondoT, Nakahara T, Kanri Y , Aoba T, Iseki S
  • Organizer: 第43回日本発生生物学会年会
  • Place of Presentation: 京都
  • Year and Date: 20100620-22
• [Book] 生物の適応放散における歯を中心とする顎顔面の器官形成と分節プラン (2010)
  • Author(s): 馬場麻人、太田正人、小澤幸重
  • Publisher: エナメル質比較発生懇話会記録
• [Book] 哺乳類の臼歯の特徴に関する一考察:異型歯性歯列における上下顎に特異的な形態形成機構 (2010)
  • Author(s): 太田正人
  • Total Pages: 78-85
  • Publisher: エナメル質比較発生懇話会記録
• [Book] 口のふしぎと歯のふしぎ(7) 味の好みが変わり、昔に比べて料理の味が濃くなりました。何か理由がありますか? (2010)
  • Author(s): 太田正人
  • Publisher: DH Styl
• [Patent(Industrial Property Rights)] Pharmaceutical co, position for prevention and/or treatment of bone disease, functional food or health food comprising the composition, and pharmaceutical preparation comprising the composition as active ingredient. (2011)
  • Inventor(s): Yonezawa Takayuki, Cha Byng-Yoon, Akazawa Hiroyuki, Truya Toshiaki, Woo Je-Tae, Ohta Masato
  • Industrial Property Rights Holder: Yonezawa Takayuki, Cha Byng-Yoon, Akazawa Hiroyuki, Truya Toshiaki, Woo Je-Tae, Ohta Masato
  • Industrial Property Number: 11184774、11184.5-2101
  • Filing Date: 2011-12-05
  • Overseas