Study on the Glu-specific protease superfamily mediating dissociation of cell-cell adhesion

2012 Fiscal Year Final Research Report

• Project/Area Number: 22592070
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Nagasaki University
• Principal Investigator: NEMOTO Yuko 長崎大学, 医歯薬学総合研究科, 准教授 (10164667)
• Co-Investigator(Kenkyū-buntansha): NEMOTO Takayuki 長崎大学, 大学院・医歯薬学総合研究科, 教授 (90164665) ; KIMURA Shigenobu 岩手医科大学, 歯学部, 教授 (10177917)
• Co-Investigator(Renkei-kenkyūsha): BABA Tomomi 長崎大学, 大学院・医歯薬学総合研究科, 助教 (60189727) ; KOBAYAKAWA Takeshi 長崎大学, 大学院・医歯薬学総合研究科, 教務職員 (10153587) ; ONO Toshio 長崎大学, 大学院・医歯薬学総合研究科, 研究員 (80050607) ; SHIMOYAMA Yu 岩手医科大学, 歯学部, 助教 (90453331)
• Project Period (FY): 2010 – 2012
• Keywords: セリンプロテアーゼ

Research Abstract

Shortening of the propeptide of Glu-specific serine peptidase(GluV8) family commonly occurred in an auto-catalytic manner. Accompanied with propeptide-shortening, the pro-mature junction became more susceptible toward the maturation enzymes. We demonstrated the in vitro Glu-specific endopeptidase activity of exfoliative toxin (ET) A for the first time, which was measured with Escherichia coli-expressed recombinant ETAs and synthetic peptidyl substrates. The animal model revealed that the proteolytic activity was indispensable for exfoliation of the skin. In addition, we determined characteristics of DPP11 and DPP7, and proposed a novel phylogenetic system of these family molecules.

Research Products

• [Journal Article] Discrimination based on Gly and Arg/Ser at Position 673 between Dipeptidyl-peptidase (DPP) 7 and DPP11, Widely Distributed DPPs in Pathogenic and Environmental Gram-Negative Bacteria (2013)
  • Author(s): Rouf, S.M.A., Ohara-Nemoto, Y., Hoshino, T., Fujiwara, T., Ono, T., Nemoto, T.K.
  • Journal Title: Biochimie Volume: 95 Pages: 824-831
  • DOI: doi:10.1016/j.biochi.2012.11.019
  • Peer Reviewed
• [Journal Article] Propeptide processing and the proteolytic activity of proenzymes of the staphylococcal and enterococcal GluV8-family protease (2012)
  • Author(s): Rouf, S.M.A., Ohara-Nemoto, Y., Shimoyama, Y., Kimura, S., Ono, T., Nemoto, T.K
  • Journal Title: Ind J Biochem Biophys Volume: 48 Pages: 421-427
  • DOI: doi:10.1016/j.biochi.2012.11.019
  • Peer Reviewed
• [Journal Article] Asp- and Glu-specific novel dipeptidyl peptidase 11 of Porphyromonas gingivalisthat ensures utilization of proteinaceous energy sources (2011)
  • Author(s): Ohara-Nemoto, Y., Shimoyama, Y., Kimura, S., Kon, A., Haraga, H., Nemoto, T.K.
  • Journal Title: J Biol Chem Volume: 286 Pages: 38115-38127
  • DOI: doi:10.1074/jbc.M111.278572
  • Peer Reviewed
• [Journal Article] Amino acid residues modulating the activities of staphylococcal glutamyl endopeptidases (2010)
  • Author(s): Ono, T., Ohara-Nemoto, Y., Shimoyama, Y., Okawara, H., Kobayakawa, T., Baba, T., Kimura, S., Nemoto, T.K.
  • Journal Title: Biol Chem Volume: 391 Pages: 1221-1232
  • DOI: doi:10.1515/BC.2010.116
  • Peer Reviewed
• [Journal Article] In vitro measurement of protease activity of exfoliative toxin A using synthetic peptide substrates.
  • Author(s): Tachi, M.T., Ohara-Nemoto, Y., Baba, T.T., Kobayakawa, T., Fujita, S., Ikeda, T., Ayuse, T., Oi, K., Nemoto, T.K.
  • Journal Title: Acta Medica Nagasakiensia Volume: (in press)
  • Peer Reviewed
• [Presentation] Dipeptide production and assimilation in Porphyromonas gingivalis by four dipeptidyl peptidases. Nagasaki University Global COE symposium (2012)
  • Author(s): Rouf, S.M.A., Ohara-Nemoto, Y., Nemoto, T.K.
  • Organizer: The 6th Nagasaki Symposium on Tropical and Emerging Infectious Diseases and The 11th Nagasaki-Singapore Medical Symposium
  • Place of Presentation: Nagasaki, Japan
  • Year and Date: 2012-12-10
• [Presentation] Expression and Characterization of Dipeptidyl- Peptidases of Porphyromonas gingivalis (2012)
  • Author(s): Rouf, S.M.A., Ohara-Nemoto, Y, Yanase, A., Ono, T., Nemoto, T.K
  • Organizer: 1st International Conference on Porphyromonas gingivalis and Related Bacterial Species
  • Place of Presentation: Nagasaki, Japan
  • Year and Date: 2012-08-27
• [Presentation] Classification and essential amino acid residues of the dipeptidyl peptidase 7/11 family in the Cytophaga- Flavobacterium-Bacteroides group (2012)
  • Author(s): Ohara-Nemoto, Y., Rouf, S.M.A., Hoshino, T., Fujiwara, T., Shimoyama, Y., Kimura, S., Ono, T., Nemoto, T.K.
  • Organizer: 112th General Meeting of ASM
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2012-06-18
• [Presentation] 表皮剥脱毒素 ETA の生物活性とプロテアーゼ活性の相関性 (2012)
  • Author(s): 達聖月,小早川健,根本孝幸,馬場友巳,根本優子
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎市
  • Year and Date: 2012-03-29
• [Presentation] Porphyromonas属菌が産生するAsp/Glu特異的新規ジペプチジルペプチダーゼ(DPP)11の同定と基質特異性の検討 (2012)
  • Author(s): 根本孝幸, SMAラオフ, 小野俊雄,下山佑,木村重信,根本優子
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎市
  • Year and Date: 2012-03-27
• [Presentation] Porphyromonas属菌が産生するAsp/Glu特異的新規ジペプチジルペプチダーゼ(DPP)11 (2011)
  • Author(s): 根本優子,下山佑,木村重信,根本孝幸
  • Organizer: 第53回歯科基礎医学学術大会
  • Place of Presentation: 岐阜市
  • Year and Date: 2011-10-01
• [Presentation] Porphyromonas gingivalisDPP11の酵素活性とAsp/Glu特異性を規定するArg670 (2011)
  • Author(s): 根本孝幸,木村重信,下山佑,木村重信,根本優子
  • Organizer: 第53回歯科基礎医学学術大会
  • Place of Presentation: 岐阜市
  • Year and Date: 2011-10-01
• [Presentation] 表皮剥奪毒素ETA N 末端領域の生物活性に及ぼす作用 (2011)
  • Author(s): 達聖月,根本優子,馬場友巳,小早川健,藤田修一,池田通,大井久美子,根本孝幸
  • Organizer: 第53回歯科基礎医学学術大会
  • Place of Presentation: 岐阜市
  • Year and Date: 2011-10-01
• [Presentation] Auto-catalytic propeptide processing facilitates the final hetero-catalytic maturation of glutamyl endopeptidase GluV8 (2011)
  • Author(s): Ohara-Nemoto, Y, Rouf, S.M.A, Shimoyama, Y., Kimura, S., Ono, T., Nemoto, T.K.
  • Organizer: 111th General Meeting of AmericanSociety for Microbiology
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2011-05-24
• [Book] NVS and staphylococci in the oral cavity, a cause of infective endocarditis (2012)
  • Author(s): Ohara-Nemoto, Y., Kimura, S., Nemoto, T.K.
  • Total Pages: 75-96
  • Publisher: In Endocarditis InTech
• [Book] Pathogenic factors of P. Gingivalis and the host defence mechanismsn Pathogenesis and Treatment of Periodontitis. (2012)
  • Author(s): Kimura, S., Ohara-Nemoto, Y., Shimoyama, Y., Ishikawa, T., Sasaki, M.
  • Total Pages: 3-18
  • Publisher: InTech
• [Remarks]
  • URL: http://www.de.nagasaki-u.ac.jp/education/dept_omb.html