2012 Fiscal Year Final Research Report
Analysis of a crosstalk signaling between mechanical stress and lyso-phospholipid signals in periodontal ligament
| Project/Area Number |
22592074
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TAKUMA Taishin 北海道医療大学, 歯学部, 教授 (40095336)
SHITARA Akiko 北海道医療大学, 歯学部, 助教 (30508718)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 歯学 / ストレス / 脂質 / シグナル伝達 / 歯根膜 |
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| Research Abstract |
LPA1 and LPA6 of LPA receptors were highly expressed; autotaxin (lyso-PLD) was also highly expressed, however, PA-PLA_ and PS-PLA_1 were not expressed. MS stimulated autotaxin mRNA in human PDL cells. ID1 (Inhibitor of DNA binding 1), EGR1 (Early growth response 1), SGK1 (Regulator of G-protein signaling 2) and DUSP1 (Dual specificity phosphatase 1) were upregulated in PDL cells. LPA produced from PDL cells was analyzed by Thin-Layer Chromatography using ^C-labeled palmitic acid and lysophosphatidylcholine. An enzyme for LPA synthesis, lyso-Phospholipase D (lyso-PLD) was induced by MS after 6h. ERK phosphorylation was determined by MS or LPA treatment at 10 min. LPA was synthesized by PDL cell with MS.
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