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2012 Fiscal Year Final Research Report

Organotypic epithelial raft cultures as HPV-related cancer models for evaluating siRNA and its delivery system

Research Project

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Project/Area Number 22592084
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathobiological dentistry/Dental radiology
Research InstitutionUniversity of Tsukuba (2012)
Tokyo Medical and Dental University (2010-2011)

Principal Investigator

YAMATO Kenji  筑波大学, 医学医療系, 研究員 (50174751)

Project Period (FY) 2010 – 2012
KeywordsHPV / RNAi / ラフトカルチャー
Research Abstract

To utilize siRNA technology to the treatment of HPV-related diseases, developing an efficient siRNA delivery system is essential. In this study, we established organotypic raft cultures using TERT- and HPV16-E6E7 immortalized human keratinocytes stably expressing firefly luciferase. These cultures facilitated quantitative analysis of knock-down efficiency induced by local siRNA delivery and showed that some cationic liposomes could deliver siRNA through stratified squamous epithelium.

  • Research Products

    (8 results)

All 2012 2011 2010

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (5 results)

  • [Journal Article] The E1 protein of human papillomavirus type 16 is dispensable for maintenance replication of the viral genome2012

    • Author(s)
      Egawa, N., Nakahara, T., Ohnoa,S., Narisawa-Saitoa,M., Yugawa,T., Fujita,M. Yamato, K., Natori Y. and Kiyono, T
    • Journal Title

      J Virol

      Volume: 86 Pages: 3276-3283

    • DOI

      DOI:10.1128/JVI.06450-11

    • Peer Reviewed
  • [Journal Article] Enhanced specificity of HPV16 E6E7 siRNA by RNA-DNA chimera modification2011

    • Author(s)
      Yamato, K., Egawa, N., Endo, S., Ui-Tei, K., Yamada, T., Saigo, K., Hyodo, I., Kiyono, T. and Nakagawa, I
    • Journal Title

      Cancer Gene Ther

      Volume: 18 Pages: 587-597

    • DOI

      DOI:10.1038/cgt.2011.28

    • Peer Reviewed
  • [Journal Article] Potent in vitro and in vivo antitumor effects of MDM2 inhibitor nutlin-3 in gastric cancer cells2011

    • Author(s)
      Endo, S., Yamato, K., Hirai, S., Moriwaki, M., Fukuda, K., Suzuki, H., Abei, M., Nakagawa, I. and Hyodo, I
    • Journal Title

      Cancer Sci

      Volume: 102 Pages: 605-613

    • DOI

      DOI:10.1111/j.1349-7006.2010.01821

    • Peer Reviewed
  • [Presentation] Identification of a short RNA segment in the siRNA seed region required for efficient RNAi2012

    • Author(s)
      大和建嗣
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡 (福岡市)
    • Year and Date
      20121211-14
  • [Presentation] ヒト胃癌細胞に対するclassIII HDAC阻害剤 tenovin-6の抗腫瘍効果に関する検討2012

    • Author(s)
      廣瀬充明
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      ロイトイン札幌(札幌
    • Year and Date
      20120919-21
  • [Presentation] The antitumor effect of HDAC inhibitor Tenovin-6 for gastric cancer cells2012

    • Author(s)
      Ueno T
    • Organizer
      American Association of Cancer Ressearch 103rd Annual Meeting
    • Place of Presentation
      Chicago, IL
    • Year and Date
      20120331-0401
  • [Presentation] AGO2-association, microRNA suppression and cytotoxicity ofRNA-DNA chimera modified siRNA2011

    • Author(s)
      大和建嗣
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      20111213-16
  • [Presentation] The anti-tumor effect of MDM2 antagonist nutlin-3 in gastric cancer cell in vitro and in vivo2010

    • Author(s)
      Endo S
    • Organizer
      American Association of Cancer Ressearch 101st Annual Meeting
    • Place of Presentation
      Washington, DC
    • Year and Date
      20100417-21

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Published: 2014-08-29  

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