2013 Fiscal Year Final Research Report
Regulation of mast cells by extracelluar matrix using self-organizing nano-technology method
| Project/Area Number |
22592099
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Tohoku Fukushi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Masaru 山形大学, 理工学研究科, 教授 (00322850)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Keywords | 肥満細胞 / 炎症 / アレルギー / ヒスタミン / ナノテクノロジー / バイオマテリアル |
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| Research Abstract |
We studied mast cell proliferation and regulation by honeycomb film (HCF). We used two types of mast cells, NCL-2 cell (new non-tumor mast cell line) and RBL-2H3 cell (rat basophilic leukemia cell line). Both of the cells cultured on 3, 5, and 10 micro m pore size of HCF revealed changing of proliferation rate, cell formation, and mediator release from mast cell though the quality of HCF is same. And then HCF regulation depended on the difference of a kind of cell. NCL-2 cell that is similar to human mucosal mast cell proliferated on the HCF, but reduced the release of TNF-alpha and leukotriene B4, which are de novo synthesized cytokines in mast cell.
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