2012 Fiscal Year Final Research Report
Degradation and down-regulation of fibrillin-1 during wound healing of human dental pulp tissue
| Project/Area Number |
22592119
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Niigata University |
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Principal Investigator |
YOSHIBA Nagako 新潟大学, 医歯学総合病院, 講師 (10323974)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIBA Kunihiko 新潟大学, 医歯学系, 准教授 (30220718)
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| Project Period (FY) |
2010 – 2012
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| Keywords | fibrillin-1 / TGF-β / dental pulp / α-SMA |
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| Research Abstract |
Degradation of fibrillins, the major constituents of microfibrils, is known to facilitate therelease of active transforming growth factor-β (TGF-β), a signaling molecule contributing tomineralized tissue barrier formation in exposed dental pulps. To examine the involvement offibrillins in the barrier formation, temporospatial expression of (i) genes and proteins offibrillins, and (ii) factors possibly associated with fibrillin degradation and cytodifferentiationwere examined in exposed human pulps. Human pulp slice cultures were also examined for therole of fibrillins in mineralization. Degradation and downregulation of fibrillin-1 expressiontook place during the mineralized tissue barrier formation in exposed pulps in vivo as well as in pulp slice cultures, which should be correlated with the temporary appearance ofα-SMA-positive fibroblasts and pulpal tissue mineralization.
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