2012 Fiscal Year Final Research Report
Capability of Regeneration Therapy with Peripheral Mononuclear BloodCells(PBMCs)
| Project/Area Number |
22592196
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HONDA Masaki 日本大学, 歯学部, 准教授 (70361623)
ISOKAWA Keitaro 日本大学, 歯学部, 教授 (50168283)
HONDA Kazuya 日本大学, 歯学部, 教授 (30199567)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 再生歯学 |
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| Research Abstract |
In vitro study, the number of cultured bone marrow cells cultured at day 3 increased in the PBMC concentration-dependently showed greater number of cells. Cell metabolic activity also increased with an increase of PBMC concentration up to by40%. Likewise, ALP activity increased at day 5 and 10 with PBMC.In radiological study, cancellous bone formation was observed from early time point and cortical bone formation was inhibited in the transplantation group. In histologicalstudy, similar results were observed. Increase of cancellous bone formation was observed in transplantation group.An addition of the PBMCs, elevated functional phenotypes of osteoprogenitor cells in vitro and in vivo. These findings warrants further in-vitro and in-vivo exploration of PBMCs as a novel source of cell therapy for bone regeneration.
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