2012 Fiscal Year Final Research Report

Development of effective methods for regeneration of the periodontal ligament of cryopreserved teeth, for expansion of indications for tooth transplantation.

Research Project

Project/Area Number	22592209
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Surgical dentistry
Research Institution	Niigata University
Principal Investigator	泉直也新潟大学, 医歯学総合病院, 助教 (10361908)
Co-Investigator(Kenkyū-buntansha)	KOJIMA Taku 新潟大学, 医歯学系, 助教 (90515777)
Project Period (FY)	2010 – 2012
Keywords	歯の移植 / 凍結保存 / 歯根膜
Research Abstract	For expansion of indications for tooth transplantation, we examined the regeneration of the periodontal tissues after cryopreservation using a rat model. The maxillarymolars of rats were extracted and transplanted into the abdominal subcutaneous tissue either immediately or after cryopreservation. The cryopreserved teeth were thawed and transplanted after having done organ culture for a certain period of time. As for the cryopreserved tooth which it transplanted after having done organ culture, the improvement of the periodontal regenerative activity was not found clearly. We intend to improve culture method and are going to determine efficacy of the organ culture of the cryopreserved tooth and technical problems in future.