Development of biosensing technology using cell-derived liposomes

2012 Fiscal Year Final Research Report

• Project/Area Number: 22650105
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Kyushu Institute of Technology
• Principal Investigator: YASUDA Takashi 九州工業大学, 大学院・生命体工学研究科, 准教授 (80270883)
• Project Period (FY): 2010 – 2012
• Keywords: リポソーム / 膜タンパク質 / GPCR / QCM / マイクロ流路 / リンパ球細胞 / バイオセンサ

Research Abstract

Liposomes were produced and separated from human lymphocytes using chemical stimulation in a microhole array devise, and the number of produced liposomesand the separation efficiency were dramatically increased. Also, liposomes havingspecific membrane proteins that were expressed in the host lymphocytes by gene transfection were immobilized on an electrode of a QCM (quartz crystal microbalance) sensor, and interactions between the membrane proteins and their ligands were detected

Research Products

• [Journal Article] 細胞由来リポソーム上の膜タンパク質を利用した QCM センサ (2013)
  • Author(s): 山中誠, 安田隆
  • Journal Title: 電気学会論文誌 Volume: Vol.133,No.5 Pages: 155-156
  • DOI: DOI: 10.1541/ieejsmas.133.155
  • Peer Reviewed
• [Presentation] QCM Detection of Membrane Protein-ligand Interactions Using Cell-derived Liposomes (2012)
  • Author(s): Makoto Yamanaka, Takashi Yasuda
  • Organizer: The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2012)
  • Place of Presentation: 沖縄コンベンションセンター(沖縄県)
  • Year and Date: 2012-10-29
• [Presentation] 細胞由来リポソームを用いた膜タンパク質-リガンド相互作用のQCM計測 (2012)
  • Author(s): 山中誠, 安田隆
  • Organizer: 第29回「センサ・マイクロマシンと応用システム」シンポジウム
  • Place of Presentation: 北九州国際会議場(福岡県)
  • Year and Date: 2012-10-24
• [Presentation] 微小孔デバイスを用いたヒト細胞由来リポソームの生成分離 (2011)
  • Author(s): 山中誠, 安田隆
  • Organizer: ナノ学会第9回大会
  • Place of Presentation: 北海道大学(北海道)
  • Year and Date: 2011-06-03
• [Presentation] Microhole Device for Derivation and Separation of Liposomes from Human Lymphocytes with Synchronized Culture (2010)
  • Author(s): Makoto Yamanaka, Takashi Yasuda
  • Organizer: The 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2010)
  • Place of Presentation: Groningen(オランダ)
  • Year and Date: 2010-10-06
• [Remarks] 研究紹介「リポソーム・デバイス」
  • URL: http://www.life.kyutech.ac.jp/ yasuda/jp/research/liposome/index.html