Can physical exercise induce differentiation from myoblasts to brown adipocytes ?

2012 Fiscal Year Final Research Report

• Project/Area Number: 22650154
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Sports science
• Research Institution: Kyorin University
• Principal Investigator: OHNO Hideki 杏林大学, 医学部, 教授 (00133819)
• Co-Investigator(Renkei-kenkyūsha): OGASAWARA Jyunetsu 杏林大学, 医学部, 助教 (20415110) ; SAKURAI Takuya 杏林大学, 医学部, 助教 (20353477) ; KIZAKI Takako 杏林大学, 医学部, 教授 (00322446)
• Project Period (FY): 2010 – 2012
• Keywords: 褐色脂肪細胞 / 水泳運動 / UCP-1 / PRDM16 / 骨格筋

Research Abstract

Recently, there has been growing evidence that PRDM16 acts as a bidirectional switch to drive the differentiation of Myf-5-expressing mesenchymal stem cells into the brown adipocytes (BA). The aim of the current study was to test whether physical exercise modifies this differential event. Male C57BL/6J mice were randomly divided into 3 groups: the sedentary control group, the acute swimming group and the cold exposed group, a positive control of BA function. Several partsof skeletal muscles were used for analysis of both mRNA and protein which might be concerned with this event. Expression of mRNA for UCP-1, a specific marker of BA, was detected only in interscapular muscles in each group. Swimming group significantly increased PRDM16 mRNA and protein levels compared with control group. Moreover, levels of PRDM16 and C/EBP-β, which is a pivotal key regulator for switching of PRDM16, were significantly higher in swimming group than in control group. These results demonstrate that swimming exercise has powerful effects on differentiation event of Myf-5-expressed myoblasts into BA, thereby suggesting that swimming exercise is clinically useful as new anti-obesitytreatment.

Research Products

• [Journal Article] Oligomerised lychee fruit-derived polyphenol attenuates cognitive impairment in senescence-accelerated mice and endoplasmic reticulum stress in neuronal cells (2013)
  • Author(s): Sakurai, T., Kitadate, K., Nishioka, H., Fujii, H., Ogasawara, J., Kizaki, T., Sato, S., Fujiwara, T., Akagawa, K., Izawa, T. & Ohno, H
  • Journal Title: Br. J. Nutr Volume: 109(in press)
  • Peer Reviewed
• [Journal Article] Role of nitric oxide in muscle regeneration following eccentric muscle contractions in rat skeletal muscle (2013)
  • Author(s): Sakurai, T., Kashimura, O., Kano, Y., Ohno, H., Li Li Ji, Izawa, T. & Best, Thomas M
  • Journal Title: J. Physiol. Sci Volume: 63(in press)
  • DOI: DOI:10.1007/s12576-013-0262-y
  • Peer Reviewed
• [Journal Article] Endogenous oxidative stress induces hypoxia-independent VEGF120 release via PI3K-dependent pathways in 3T3-L1 adipocytes (2013)
  • Author(s): Takahashi, K., Miyokawa-Gorin, K., Handa, K., Kitahara, A., Moriya, R., Onuma, H., Sumitani, Y., Tanaka, T., Katsuta, H., Nishida, S., Ohno, H. & Ishida, H
  • Journal Title: Obesity Volume: 21(in press)
  • Peer Reviewed
• [Journal Article] Intracellular β2-adrenergic receptor signaling specificity in mouse skeletal muscle in response to single-dose β2-agonist clenbuterol treatment and acute exercise (2013)
  • Author(s): Sato, S., Shirato, K., Mitsuhashi, R., Inoue, D., Kizaki, T., Ohno, H., Tachiyashiki, K. & Imaizumi, K
  • Journal Title: J. Physiol. Sci Volume: 63 Pages: 211-218
  • DOI: DOI :10.1007/s12576-013-0253-z
  • Peer Reviewed
• [Journal Article] Redox- regulating sirtuins in aging, caloric restriction, and exercise (2013)
  • Author(s): Radak, Z., Koltai, E., Taylora, AW., Higuchi, M., Kumagai, S., Ohno, H., Goto, S. & Boldogh, I
  • Journal Title: Free Radic. Biol. Med Volume: 58 Pages: 87-97
  • DOI: DOI:10.1016/j.freeradbiomed.2013. 01.004
  • Peer Reviewed
• [Journal Article] Oxygen consumption and usage during physical exercise: The balance between oxidative stress and ROS-dependent adaptive signaling (2013)
  • Author(s): Radak, Z., Zhao, Z., Koltai, E., Ohno, H. & Atalay, M
  • Journal Title: Antioxid Redox Signal Volume: 18 Pages: 1208-1246
  • DOI: DOI:10.1089/ars.2011.4498
  • Peer Reviewed
• [Journal Article] Oligonol-induced degradation of perilipin 1 is regulated through lysosomal degradation machinery (2012)
  • Author(s): Ogasawara, J., Kitadate, K., Nishioka, H., Fujii, H., Sakurai, T., Kizaki, T., Izawa, T., Ishida, H. & Ohno, H
  • Journal Title: Nat. Prod. Commun Volume: 7 Pages: 1193-1196
  • Peer Reviewed
• [Journal Article] Higher levels of ATGL are associated with exercise-induced enhancement of lipolysis in rat epididymal adipocytes (2012)
  • Author(s): Ogasawara, J., Sakurai, T., Kizaki, T., Ishibashi, Y., Izawa, T., Sumitani, Y., Ishida, H., Radak, Z., Haga, S. & Ohno, H
  • Journal Title: PLoS One Volume: 7 Pages: e40876
  • DOI: DOI:10.1371/journal.pone.0040876
  • Peer Reviewed
• [Journal Article] Recent advances in the adaptations of adipose tissue to physical activity : Morphology and adipose tissue cellularity (2012)
  • Author(s): Izawa, T., Ogasawara, J., Sakurai, T., Nomura, S., Kizaki, T. & Ohno, H
  • Journal Title: J. Phys. Fit. Sports Med Volume: 1 Pages: 381-387
  • Peer Reviewed
• [Journal Article] 登山に対する電気伝導率を利用した疲労評価 (2012)
  • Author(s): 内藤祐二郎、長澤純一、杉山康司、笹尾真美、野口いづみ、佐藤章悟、石橋義永、木崎節子、大野秀樹
  • Journal Title: 登山医学 Volume: 32 Pages: 148-154
  • Peer Reviewed
• [Journal Article] Effect of exercise on HIF-1 and VEGF signaling (2012)
  • Author(s): Ohno, H., Shirato, K., Sakurai, T., Ogasawara, J., Sumitani, Y., Sato, S., Imaizumi, K., Ishida, H. & Kizaki, T
  • Journal Title: J. Phys. Fit. Sports Med Volume: 1 Pages: 5-16
  • Peer Reviewed
• [Journal Article] Copy number variations due to large genomic deletion in x-linked chronic granulomatous disease (2012)
  • Author(s): Arai, T., Oh-Ishi, T., Yamamoto, H., Nunoi, H., Kamizono, J., Uehara, M., Kubota, T., Sakurai, T., Kizaki, T. & Ohno, H
  • Journal Title: PLoS One Volume: 7 Pages: e27782
  • DOI: DOI :10.1371/journal.pone.0027782
  • Peer Reviewed
• [Journal Article] Preventive and improvement effects of exercise training and supplement intake in white adipose tissues on obesity and lifestyle- related diseases (2012)
  • Author(s): Sakurai, T., Ogasawara, J., Kizaki, T., Ishibashi, Y., Sumitani, Y., Takahashi, K., Ishida, H., Miyazaki, H., Saitoh, D., Haga, S., Izawa, T. & Ohno, H
  • Journal Title: Environ. Health Prev. Med Volume: 17 Pages: 348-356
  • DOI: DOI :10.1007/s12199-012-0271-0
  • Peer Reviewed
• [Journal Article] The effects of exercise on macrophage function (2012)
  • Author(s): Kizaki, T., Sato, S., Sakurai, T., Ogasawara, J., Imaizumi, K., Izawa, T. Nagasawa, J., Saitoh, D., Haga, S. & Ohno, H
  • Journal Title: J. Phys. Fit. Sports Med Volume: 1 Pages: 113-124
  • Peer Reviewed
• [Journal Article] Exercise training and the promotion of neurogenesis and neurite outgrowth in the hippocampus (2012)
  • Author(s): Sakurai, T., Ogasawara, J., Kizaki, T.,Ishibashi, Y., Fujiwara, T., Akagawa, K., Izawa, T., Radak, Z. & Ohno, H
  • Journal Title: J. Phys. Fit. Sports Med Volume: 1 Pages: 333-338
  • Peer Reviewed
• [Journal Article] Effect of physical exercise on lipolysis in white adipocytes (2012)
  • Author(s): Ogasawara, J., Sakurai, T., Kizaki, T., Takahashi, K., Sumitani, Y., Ishida, H., Izawa, T., Toshinai, K., Nakano, N. & Ohno, H
  • Journal Title: J. Phys. Fit. Sports Med Volume: 1 Pages: 351-356
  • Peer Reviewed
• [Journal Article] Effects of β2 - agonists and exercise on β2 - adrenergic receptor signaling in skeletal muscles (2012)
  • Author(s): Sato, S., Shirato, K., Kizaki, T., Ohno, H., Tachiyashiki, K. & Imaizumi, K
  • Journal Title: J. Phys. Fit. Sports Med Volume: 1 Pages: 139-144
  • Peer Reviewed
• [Journal Article] Effects of exercise on the hexosamine biosynthetic pathway and glycosylation (2012)
  • Author(s): Shirato, K., Kizaki, T., Ohno, H. & Imaizumi, K
  • Journal Title: J. Phys. Fit. Sports Med Volume: 1 Pages: 145-150
  • Peer Reviewed
• [Journal Article] Induction of mitochondrial uncoupling enhances VEGF (120) but reduces MCP-1 release in mature 3T3-L1 adipocytes: Possible regulatory mechanism through endogenous ER stress and AMPK-related pathways (2012)
  • Author(s): Miyokawa-Gorin, K., Takahashi, K., Handa, K., Kitahara, A., Sumitani, Y., Katsuta, H., Tanaka, T., Nishida, S., Yoshimoto, K., Ohno, H. & Ishida, H
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 419 Pages: 200-205
  • DOI: DOI :10.1016/j.bbrc.2012.01.145
  • Peer Reviewed
• [Journal Article] Influence of mitochondrial DNA 5178 C/A polymorphism on serum cholesterol changes : a short-term follow-up in middle-aged Japanese men (2012)
  • Author(s): Honmyo, R., Kokaze, A., Karita, K., Yoshida, M., Ishikawa, M. & Ohno, H
  • Journal Title: Environ. Health Prev. Med Volume: 17 Pages: 401-407
  • DOI: DOI :10.1007/s12199-012-0268-8
  • Peer Reviewed
• [Journal Article] Erythropoietin, 2,3 DPG, oxygen transport capacity, and altitude training in adolescent Alpine skiers (2012)
  • Author(s): Son, HJ., Kim, HJ., Kim, JH., Ohno, H. & Kim, CK
  • Journal Title: Aviat. Space Environ. Med Volume: 83 Pages: 50-53
  • Peer Reviewed
• [Journal Article] Involvement of leucine zipper transcription factor-like protein 1 (Lztfl1) in the attenuation of cognitive impairment by exercise training (2011)
  • Author(s): Sakurai, T., Ogasawara, J., Kizaki, T., Ishibashi, Y., Fujiwara, T., Akagawa, K., Izawa, T., Oh-ishi, S., Haga, S. & Ohno, H
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 416 Pages: 125-129
  • DOI: DOI :10.1016/j.bbrc.2011.11.008
  • Peer Reviewed
• [Journal Article] Voluntary exercise attenuates obesity-associated inflammation through ghrelin expressed in macrophages (2011)
  • Author(s): Kizaki, T., Maegawa, T., Sakurai, T., Ogasawara, J., Ookawara, T., Oh-ishi, S., Izawa, T., Haga, S., & Ohno, H
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 413 Pages: 454-459
  • DOI: DOI :10.1016/j.bbrc.2011.08.117
  • Peer Reviewed
• [Journal Article] Augmented bacterial elimination by Kupffer cells after IL-18 pretreatment via IFN-γ produced from NK cells in burn-injured mice (2011)
  • Author(s): Miyazaki, H., Kinoshita, M., Ono, S., Nakashima, M., Hara, E., Ohno, H., Seki, S. & Saitoh, D
  • Journal Title: Burns Volume: 37 Pages: 1208-1215
  • DOI: DOI :10.1016/j.burns.2011.04.010
  • Peer Reviewed
• [Journal Article] 富士登山時の自律神経応答と身体の電気伝導率 (2011)
  • Author(s): 長澤純一、内藤祐二郎、杉山康司、笹尾 真美、曽我部健、新美敬太、野口いづみ、芳賀脩光、石橋義永、木崎節子、大野秀樹
  • Journal Title: 登山医学 Volume: 31 Pages: 145-152
  • Peer Reviewed
• [Journal Article] 高度が味覚感度へ及ぼす影響-富士山における味覚定性定量検査- (2011)
  • Author(s): 笹尾真美、野口いづみ、新美敬太、曽我部健、井出正道、高野宏二、杉山康司、長澤純一、大野秀樹
  • Journal Title: 登山医学 Volume: 31 Pages: 127-131
  • Peer Reviewed
• [Journal Article] Effect of exercise training on the density of endothelial cells in the white adipose tissue of rats (2011)
  • Author(s): Hatano, D., Ogasawara, J., Endoh, S., Sakurai, T., Nomura, S., Kizaki, T., Ohno, H., Komabayashi, T. & Izawa, T
  • Journal Title: Scand. J. Med. Sci. Sports Volume: 21 Pages: e115-e121
  • DOI: DOI :10.1111/j.1600-0838.2010.01176. x
  • Peer Reviewed
• [Journal Article] Comparison of the effect of oligonol, a new lychee fruit-derived low molecular form of polyphenol, and epigallocatechin- 3-gallate on lipolysis in rat primary adipocytes (2011)
  • Author(s): Ogasawara, J., Kitadate, K., Nishioka, H., Fujii, H., Sakurai, T., Kizaki, T., Izawa, T., Ishida, H. & Ohno, H
  • Journal Title: Phytother Res Volume: 25 Pages: 467-471
  • DOI: DOI :10.1002/ptr.3296
  • Peer Reviewed
• [Journal Article] Hormone-sensitive lipase is critical mediators of acute exercise-induced regulation of lipolysis in rat adipocytes (2010)
  • Author(s): Ogasawara, J., Nomura, S., Rahman, N., Sakurai, T., Kizaki, T., Izawa, T., Ishida, H., Haga, S. & Ohno, H
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 400 Pages: 134-139
  • DOI: DOI :10.1016/j.bbrc.2010.08.026
  • Peer Reviewed
• [Journal Article] Oligonol, an oligomerized lychee fruit-derived polyphenol, activates the Ras/Raf-1/MEK1/2 cascade independent of the IL-6 signaling pathway in rat primary adipocytes (2010)
  • Author(s): Ogasawara, J., Kitadate, K., Nishioka, H., Fujii, H., Sakurai, T., Kizaki,T., Izawa, T., Ishida, H., Tanno, M. & Ohno, H
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 402 Pages: 554-559
  • DOI: DOI :10.1016/j.bbrc.2010.10.082
  • Peer Reviewed
• [Journal Article] Effects of exercise training on adipogenesis of stromal-vascular fraction cells in rat epididymal white adipose tissue (2010)
  • Author(s): Sakurai, T., Endo, S., Hatano, D., Ogasawara, J., Kizaki, T., Oh-ishi, S., Izawa, T., Ishida, H. & Ohno, H
  • Journal Title: Acta Physiol (Oxf) Volume: 200 Pages: 325-338
  • DOI: DOI :10.1111/j.1748-1708.2010.02159.x
  • Peer Reviewed
• [Journal Article] 低圧・低酸素環境が引き起こす酸化ストレスとオリゴノールの抗酸化効果 (2010)
  • Author(s): 長澤純一、杉山康塚、内丸仁、笹尾真美、高野宏二、野口いづみ、鈴木康弘、 北舘健太郎、芳賀脩光、前川剛輝、櫻井拓也、小笠原準悦、木崎節子、大野秀樹
  • Journal Title: 登山医学 Volume: 30 Pages: 118-124
  • Peer Reviewed
• [Presentation] 水泳運動により誘導さ れる肩甲骨周囲骨格筋群の褐色脂肪細胞 化の検討 (2013)
  • Author(s): 小笠原準悦ほか
  • Organizer: 第83回日本衛生学会学術総会
  • Place of Presentation: 金沢
  • Year and Date: 2013-03-25
• [Presentation] 運動すると なぜ痩せる?～白色脂肪細胞ではいった い何がおこるのか～ (2012)
  • Author(s): 小笠原準悦ほか
  • Organizer: 第14回日本体力医 学会北海道地方会
  • Place of Presentation: 札幌
  • Year and Date: 2012-06-17
• [Presentation] 水泳運動は骨格筋前駆 細胞から褐色脂肪細胞への分化シグナルを促進する (2012)
  • Author(s): 小笠原準悦ほか
  • Organizer: 第82回日本衛生学会学術総会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-25
• [Presentation] 骨格筋から褐色脂肪細 胞への分化機構に及ぼす寒冷曝露と水泳 運動の効果 (2011)
  • Author(s): 小笠原準悦ほか
  • Organizer: 第66回日本体力医学会大会
  • Place of Presentation: 下関
  • Year and Date: 2011-09-17
• [Presentation] The lipolytic activity in adipocytes differs between normal and high fat diet mice via changes in the trafficking of β2- adrenergicreceptor (2011)
  • Author(s): Ogasawara, J. et al
  • Organizer: 第88回日本生理 学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-30
• [Presentation] Oligonol, a new lychee fruit-derived low molecular form of polyphenol, enhances lipolysis in primary rat adipocytes via adtivation of the ERK1/2 pathway (2010)
  • Author(s): Ogasawara, J. et al
  • Organizer: 11th International Congress on Obesity
  • Place of Presentation: Stochholm (Sweden)
  • Year and Date: 2010-07-13
• [Book] 機能性食品素材と運動療法-生活習慣病予防と運動機能維持向上を目指して- (2012)
  • Author(s): 大野秀樹ほか
  • Total Pages: 177-184
  • Publisher: シーエムシー出版
• [Book] からだと温度の事典 (2010)
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  • Total Pages: 516-517
  • Publisher: 朝倉書店