2011 Fiscal Year Final Research Report
Exploring the possibilities of genome recombination
Project/Area Number |
22655053
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Single-year Grants |
Research Field |
Chemistry related to living body
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Research Institution | Kyoto University |
Principal Investigator |
ATOMI Haruyuki 京都大学, 大学院・工学研究科, 教授 (90243047)
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Project Period (FY) |
2010 – 2011
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Keywords | 遺伝子組換え / 超好熱菌 / メタゲノム / ゲノム組換え / 合成生物学 |
Research Abstract |
Focusing on the high transformation efficiency of the hyperthermophilic archaeon Thermococcus kodakarensis, we explored the possibilities of performing wide-scale DNA recombination. Mutant strains displaying uracil or tryptophan auxotrophy were used as host cells, and selection was based on their prototrophy. We were able to obtain prototrophs using the genomic DNA of wild-type T. kodakarensis. By inserting a small DNA fragment from the DNA donor beforehand, we were able to obtain transformants using genomic DNA from other hyperthermophiles.
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Research Products
(9 results)
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[Journal Article] Insights into the evolution of Archaea and eukaryotic protein modifier systems revealed by the genome of a novel archaeal group2011
Author(s)
Nunoura T, Takaki Y, Kakuta J, Nishi S, Sugahara J, Kazama H, Chee G-J, Hattori M, Kanai A, Atomi H, Takai K, Takami H
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Journal Title
Nucleic Acids Research
Volume: 39
Pages: 3204-3223
Peer Reviewed
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[Journal Article] His tone and TK0471/TrmBL2 form a novel heterogeneous genome architecture in the hyperthermophilic archaeon The rmococcus kodakarensis2011
Author(s)
Maruyama, H., Shin, M., Oda, T., Matsumi, R., Ohniwa, R. L., Itoh, T., Shirahige, K., Imanaka, T., Atomi, H., Yoshimura, S. H., Takeyasu, K
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Journal Title
Molecular Biology of the Cell
Volume: 22(3)
Pages: 386-398
Peer Reviewed
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[Journal Article] For mate-driven growth coupled with hydrogen production2010
Author(s)
Kim, Y. J., Lee, H. S., Kim, E. S., Bae, S. S., Lim, J. K., Matsumi, R., Lebedinsky, A. V., Sokolova, T. G., Kozhevnikova, D. A., Cha, S. S., Kim, S. J., Kwon, K. K., Imanaka, T., Atomi, H., Bonch-Osmolovskaya, E. A., Lee, J. H., Kang, S. G
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Journal Title
Nature
Volume: 497(7313)
Pages: 352-355
Peer Reviewed
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